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Akademický článek

Functional and Structural Characterization of Diverse NfsB Chloramphenicol Reductase Enzymes from Human Pathogens

Autor: Michael W. Mullowney, Natalia I. Maltseva, Michael Endres, Youngchang Kim, Andrzej Joachimiak, Terence S. Crofts

Publikováno v: Microbiology Spectrum, Vol 10, Iss 2 (2022)

ABSTRACT Phylogenetically diverse bacteria can carry out chloramphenicol reduction, but only a single enzyme has been described that efficiently catalyzes this reaction, the NfsB nitroreductase from Haemophilus influenzae strain KW20. Here, we tested

Externí odkaz: https://doaj.org/article/10083284a0c145cdad571bcd81c4d296

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Akademický článek

Mosaic Ends Tagmentation (METa) Assembly for Highly Efficient Construction of Functional Metagenomic Libraries

Autor: Terence S. Crofts, Alexander G. McFarland, Erica M. Hartmann

Publikováno v: mSystems, Vol 6, Iss 3 (2021)

Medically and industrially important genes can be recovered from microbial communities by high-throughput sequencing, but precise annotation is often limited to characterized genes and their relatives. Cloning a metagenome en masse

Externí odkaz: https://doaj.org/article/4783ac75e8874e768426eb7ac681cb7f

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