Zobrazeno 1 - 10
of 61
pro vyhledávání: '"S. B. Bokut"'
Autor:
B. A. Bushuk, O. S. Bokut, V. E. Syakhovich, A. S. Drazdou, E. I. Kvasyuk, A. N. Sobchuk, S. B. Bokut
Publikováno v:
Journal of Applied Spectroscopy. 79:437-445
We have studied complex formation between molecules of the fluorescent probe 1-anilinonaphthalene-8-sulfonate (1,8-ANS) and the major form (A1) and a minor form (Ab) of hemoglobin. The contribution of the longlived component f3 to the kinetic curves
Autor:
A. A. Milyutin, Boris M. Dzhagarov, S. B. Bokut, Nicolai A. Nemkovich, P. A. Kisselev, D. A. Parul, Eugene P. Petrov, A. N. Sobchuk
Publikováno v:
Biochemistry (Moscow). 66:390-396
Binding of 1,8-anilinonaphthalene sulfonate (1,8-ANS) with native human oxyhemoglobin (Hb) in 50 mM potassium phosphate buffer (pH 7.4) was studied by steady state fluorescence spectroscopy and by laser spectrofluorimetry with subnanosecond time reso
Autor:
D. A. Parul, Nicolai A. Nemkovich, A. N. Sobchuk, A. A. Milyutin, Boris M. Dzhagarov, Eugene P. Petrov, S. B. Bokut
Publikováno v:
Journal of Photochemistry and Photobiology B: Biology. 58:156-162
Time-resolved fluorescence of 1,8-anilinonaphthalene sulfonate (1,8-ANS) fluorescent probe bound to intact human oxyhemoglobin (HbO2) is investigated. Fluorescence emission spectra of 1,8-ANS in a potassium buffer solution (pH 7.4) of HbO2 undergo a
Autor:
Vladimir N. Barai, A. I. Zinchenko, E. V. Vaaks, E. I. Kvasyuk, S. B. Bokut, G. V. Zaitseva, Igor A. Mikhailopulo
Publikováno v:
Nucleosides and Nucleotides. 13:819-834
Treatment of O2, 3′-anhydro-5′-O-trityl derivatives of thymidine (1) and 2′-deoxyuridine (2) with lithium azide in dimethylformamide at 150 °C resulted in the formation of the corresponding isomeric 3′-azido-2′, 3′-dideoxy-5′-O-trityl-
Autor:
A. I. Zinchenko, Elena N. Kalinichenko, Igor A. Mikhailopulo, S. B. Bokut, Vladimir N. Barai, Zygmunt Kazimierczuk
Publikováno v:
Nucleosides and Nucleotides. 12:417-422
The title compound have been synthesized by an enzymatic trans-2′-deoxyribosylation of 2-chloroadenine using the whole cells of E. coli BMT-1D/1A as a biocatalyst and 2′-deoxyguanosine as a donor of glycosyl moiety.
Autor:
S. B. Bokut, Frank Seela, Igor A. Mikhailopulo, N. V. Dudchik, Elena N. Kalinichenko, A. I. Zinchenko, V. N. Baraj, Helmut Rosemeyer
Publikováno v:
Biotechnology Letters. 14:885-890
Substrate activity of 1- and 3-deazapurines in the reaction of microbiological ribo- 2-deoxyribosylation catalysed by purine nucleoside phosphorylase of viable cells ofE. coli BMT-1D/1A has been studied. Guanosine or 2′-deoxyguanosine were used as
Autor:
S. B. Bokut, A. I. Zinchenko, E. I. Kvasyuk, Igor A. Mikhailopulo, N. V. Dudchik, Vladimir N. Barai
Publikováno v:
Biotechnology Letters. 13:87-90
The title compound was prepared by a two step enzymatic procedure consisting of DNA hydrolysis to the mixture of 2′-deoxynucleosides followed by a transdeoxyribosilation of exogenous adenine.
Autor:
Vladimir N. Barai, Yu. V. Belyaeva, S. B. Bokut, Igor A. Mikhailopulo, N. V. Dudochik, A. J. Zinchenko
Publikováno v:
Biotechnology Letters. 12:341-346
2′-Deoxythymidine was synthesized by an enzymatic transdeoxyribosylation of thymine using either (i) dGuo, dCyd or dAdo, or (ii) the mixture of the same 2′-deoxynucleosides resulting from enzymatic hydrolysis of DNA as donors of 2-deoxyribofurano
Publikováno v:
Applied Microbiology and Biotechnology. 32:658-661
Synthesis of 9-(beta-D-arabinofuranosyl)guanine (ara-G) from 1-(beta-D-arabinofuranosyl)cytosine (ara-C) and guanine, guanosine or 2'-deoxyguanosine (dG) by glutaraldehyde-treated Escherichia coli BM-11 cells is described. It is shown that the concen
Autor:
D A, Parul, S B, Bokut, P A, Kisselev, A A, Milyutin, E P, Petrov, N A, Nemkovich, A N, Sobchuk, B M, Dzhagarov
Publikováno v:
Biochemistry. Biokhimiia. 66(4)
Binding of 1,8-anilinonaphthalene sulfonate (1,8-ANS) with native human oxyhemoglobin (Hb) in 50 mM potassium phosphate buffer (pH 7.4) was studied by steady-state fluorescence spectroscopy and by laser spectrofluorimetry with subnanosecond time reso