Zobrazeno 1 - 7
of 7
pro vyhledávání: '"S. Akram Shirdel"'
Publikováno v:
Archives of biochemistry and biophysics. 668
Chondroitinase ABC I (cABC I) can degrade inhibitory molecules for axon regrowth at the site of damage after spinal cord injury (SCI). One of the main problems in the practical application is the possibility of structural changes that lead to the ina
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b4993019bf190a6c693fbdd4f7605386
https://pubmed.ncbi.nlm.nih.gov/31103558
https://pubmed.ncbi.nlm.nih.gov/31103558
Autor:
Abolfazl Golestani, Vahab Jafarian, S. Shahrbanoo Jafari, Masoumeh Shamsi, S. Akram Shirdel, Khosrow Khalifeh
Publikováno v:
Engineering in Life Sciences. 16:690-696
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1b9cf6c2a3901e7a7ac0af4867ce47bb
https://doi.org/10.1002/elsc.201600034
https://doi.org/10.1002/elsc.201600034
Publikováno v:
RSC Advances. 6:59834-59841
To identify the structural and functional roles of Glu9 located at the first loop of sweet-tasting brazzein, two different mutants (E9K and E9G) of the minor form of brazzein were designed and constructed. Upon histidine-tag (His-tag) elimination and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b4bc1575c98efef4af457a8efc1d0c72
https://doi.org/10.1039/c6ra12626j
https://doi.org/10.1039/c6ra12626j
Publikováno v:
Engineering in Life Sciences. 14:229-234
Neutral proteases are inactivated at higher temperatures because of autolysis. It appears that autolysis involves some specific solvent-exposed regions that become prone to local unfolding as temperature increases. Accordingly, we designed surface-lo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4d8d2c15ff98380a3f49ca904c99d46a
https://doi.org/10.1002/elsc.201200218
https://doi.org/10.1002/elsc.201200218
Publikováno v:
Enzyme and microbial technology.
We had previously investigated the role of a loop on the activity and conformational stability of chondroitinase ABC Ι (cABC Ι) by constructing some representative mutants in which a network interaction around Asp 689 was manipulated. Here we exten
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48f25f50fde5fcc6d4ac31b2ee1dff01
https://pubmed.ncbi.nlm.nih.gov/27702482
https://pubmed.ncbi.nlm.nih.gov/27702482
Publikováno v:
Molecular biotechnology. 57(8)
We used a combination of protein engineering and spectroscopic methods to investigate the effect of a long length loop on the conformational stability and activity of chondroitinase ABC I. This study involves manipulation of interactions around Asp(6
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f3f727508ff4e0a6e1bfef4937ed266
https://pubmed.ncbi.nlm.nih.gov/25808032
https://pubmed.ncbi.nlm.nih.gov/25808032
Autor:
Akram Shirdel S; Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran., Khalifeh K, Golestani A, Ranjbar B, Khajeh K
Publikováno v:
Molecular biotechnology [Mol Biotechnol] 2015 Aug; Vol. 57 (8), pp. 727-34.