Cytosolic expression of functional Fab fragments in Escherichia coli using a novel combination of dual SUMO expression cassette and EnBase® cultivation mode

Autor: Noushin Davoudi, K. Mansouri, Fatemeh Davami, mahdiyeh azizi, Ramin Fazel, Somayeh Enayati, Vahid Khalaj, S. Agha Amiri, Reza Mahdian, Faegheh Rezaie

Publikováno v: Journal of Applied Microbiology. 123:134-144

Aims The Escherichia coli expression system is highly effective in producing recombinant proteins. However, there are some limitations in this system, especially in obtaining correctly folded forms of some complex proteins such as Fab fragments. To i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f32f905707c7a2f96e9cd02fc00715ef
https://doi.org/10.1111/jam.13483

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Identification and Evaluation of Novel Drug Targets against the Human Fungal Pathogen Aspergillus fumigatus with Elaboration on the Possible Role of RNA-Binding Protein.

Autor: Malekzadeh S; Fungal Biotechnology Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran.; Bioinformatics and Drug Design Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran., Sardari S; Bioinformatics and Drug Design Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran., Azerang P; Bioinformatics and Drug Design Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran., Khorasanizadeh D; Fungal Biotechnology Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran., Agha Amiri S; Fungal Biotechnology Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran., Azizi M; Fungal Biotechnology Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran., Mohajerani N; Fungal Biotechnology Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran., Khalaj V; Fungal Biotechnology Unit, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran 1316943551, Iran.

Publikováno v: Iranian biomedical journal [Iran Biomed J] 2017 Mar; Vol. 21 (2), pp. 84-93. Date of Electronic Publication: 2016 Dec 21.