Zobrazeno 1 - 10
of 10
pro vyhledávání: '"S. A. Cherepushkin"'
Autor:
S. A. Cherepushkin, V. V. Tsibezov, A. G. Yuzhakov, O. E. Latyshev, K. P. Alekseev, E. G. Altayeva, K. M. Khametova, G. K. Vorkunova, K. A. Yuzhakova, T. V. Grebennikova
Publikováno v:
Вопросы вирусологии, Vol 66, Iss 1, Pp 55-64 (2021)
Introduction. Rotavirus infection is the leading cause of acute gastroenteritis among infants. The development of new vaccines against rotavirus A is urgent because the virus has many genotypes, some of which have regional prevalence. Virus-like part
Externí odkaz:
https://doaj.org/article/c8f1323ac5b040cd80bf87522cc2e265
Publikováno v:
Разработка и регистрация лекарственных средств, Vol 0, Iss 3, Pp 124-129 (2019)
Method of determination of polysorbate 20 and polysorbate 80 in recombinant protein drugs based on spectrophotometry has been designed. Isolation of polysorbate from solution is carried out by solid phase extraction followed by elution of polysorbate
Externí odkaz:
https://doaj.org/article/841b8c3eb6fc4e55bb23675a9958b18f
Publikováno v:
Биопрепараты: Профилактика, диагностика, лечение, Vol 16, Iss 2, Pp 120-124 (2018)
The method for the assessment of biological activity of erythropoietin preparations in vitro on TF-1 sensitive cells culture has been developed. The special characteristics of measurements and calculations of the biological activity of erythropoietin
Externí odkaz:
https://doaj.org/article/4b97b448c2a94e89b078c7cb3b7859c5
Autor:
E G Altayeva, K P Alekseev, G K Vorkunova, T. V. Grebennikova, V V Tsibezov, Oleg Latyshev, Kizkhalum M. Khametova, S A Cherepushkin, A G Yuzhakov, K A Yuzhakova
Publikováno v:
Problems of Virology. 66:55-64
Introduction. Rotavirus infection is the leading cause of acute gastroenteritis among infants. The development of new vaccines against rotavirus A is urgent because the virus has many genotypes, some of which have regional prevalence. Virus-like part
Autor:
P A Orlova, M. S. Poponova, S A Cherepushkin, R A Marigin, E V Voronina, N V Strukova, A. V. Grishin, Maria S. Generalova, Lyubov’ A. Soboleva, I. S. Boksha, Vasily N. Manskikh, K E Nikitin, A. V. Gromov, Anna S. Karyagina, A M Lyaschuk, I N Trusova, T. M. Grunina
Publikováno v:
Biochemistry. Biokhimiia. 84(1)
The aim of this work was to compare biological activities of three variants of bacterially expressed human recombinant erythropoietin (EPO) with additional protein domains: 6His-s-tag-EPO protein carrying the s-tag (15-a.a. oligopeptide from bovine p
Autor:
Vasily N. Manskikh, Anna S. Karyagina, Vladimir G. Lunin, M S Manukhina, N. B. Polyakov, P A Orlova, M. S. Poponova, Z. M. Galushkina, V G Zhukhovitsky, A V Demidenko, Andrey I. Solovyev, I. S. Boksha, S A Cherepushkin, D A Tretyak, N V Strukova, A. V. Gromov, A M Lyaschuk, K E Nikitin, T. M. Grunina
Publikováno v:
Biochemistry. Biokhimiia. 83(10)
Recombinant human erythropoietin (EPO) with additional N-terminal heparin-binding protein domain (HBD) from bone morphogenetic protein 2 was synthesized in Escherichia coli cells. A procedure for HBD-EPO purification and refolding was developed for o
Publikováno v:
Applied Biochemistry and Microbiology. 51:818-826
The recombinant E7 oncoprotein gene of type 16 human papilloma virus (conjugated with the heat shock protein 70 (E7(16)-HSP70)) was expressed in Saccharomyces cerevisiae yeast cells. A number of chromatographic isolations were conducted to obtain the
Publikováno v:
Biotekhnologiya. :29-37
A gene for a recombinant E7 oncoprotein of human papillomavirus type 16 conjugated with a heat shock protein 70 (E7(16)-HSP70) has been expressed in Saccharomyces cerevisiae. A number of chromatographic separations was performed in order to primarily
Publikováno v:
Biotekhnologiya. :54-63
A nickel-chelate affinity chromatography has been used to isolate fused proteins of Hsp70-E7 from yeast lysate at the initial stage of the work. The low purity of the monomeric Hsp70-E7 in the final fraction was associated with the occurrence of dime
Publikováno v:
Russian Journal of Ecology. 36:135-137