ISOLATION OF Clq-BINDING IMMUNE COMPLEXES BY AFFINITY CHROMATOGRAPHY AND DESORPTION WITH A DIAMINOALKYL COMPOUND

Autor: D. Burger, S.-E. Svehag

Publikováno v: Acta Pathologica Microbiologica Scandinavica Section C Immunology. :45-52

The applicability of affinity chromatography to the isolation of Clq-binding immune complexes (IC) in sera was explored. Purified human Clq was covalently coupled to agarose or adsorbed to IgG-agarose resins. Sera containing preformed virus-antibody

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f50c3d254adc7b117c7922dc777808b5
https://doi.org/10.1111/j.1699-0463.1976.tb03598.x

Isolation and Characterization of Porcine Mannan‐Binding Proteins of Different Size and Ultrastructure

Autor: Uffe Holmskov, Ove Andersen, P. Storgaard, E. Skriver, Peter Højrup, E. Holm Nielsen, H. Mortensen, S.-E. Svehag, G. Leslie

Publikováno v: Scandinavian Journal of Immunology. 43:289-296

The authors report on the purification and characterization of mannan- binding proteins (MBP) isolated from porcine serum. The MBPs were purified by use of PEG precipitation, affinity chromatography on mannan-Sepharose, protein A- and anti-porcine Ig

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::616ead270e2e44269924b66fdbe95bbb
https://doi.org/10.1046/j.1365-3083.1996.d01-39.x

Complement receptor expression and activation of the complement cascade on B lymphocytes from patients with systemic lupus erythematosus (SLE)

Autor: Peter Junker, R. G. Q. Leslie, Anders Jørgen Svendsen, S E Svehag, J M Rasmussen, Claus Henrik Nielsen, H. V. Marquart

Publikováno v: Marquart, H V, Svendsen, A J, Rasmussen, J M, Nielsen, C H, Junker, P, Svehag, S E & Leslie, R G 1995, ' Complement receptor expression and activation of the complement cascade on B lymphocytes from patients with systemic lupus erythematosus (SLE) ', Clinical and Experimental Immunology, vol. 101, no. 1, pp. 60-65 . https://doi.org/10.1111/j.1365-2249.1995.tb02277.x

It has previously been reported that the expression of the complement receptors, CR1 on erythrocytes and blood leucocytes and CR2 on B cells, is reduced in patients with SLE, and that the reduced expression of CR1 on erythrocytes is related to diseas

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d357f8e8866370f37faba8fe092be163
https://doi.org/10.1111/j.1365-2249.1995.tb02277.x

Conglutinin Binds The HIV-1 Envelope Glycoprotein gpl60 and Inhibits its Interaction with Cell Membrane CD4

Autor: S.‐E. Svehag, Ove Andersen, A.‐M. Sørensen, E. Fenouillet

Publikováno v: Scandinavian Journal of Immunology. 33:81-88

The highly glycosylated envelope glycoprotein (gp 160) of human immunodeficiency virus (HIV) interacts with the CD4 molecule present on the membrane of CD4+ cells and is involved in the pathobiology of HIV infection. Lectins bind glycoproteins throug

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::3f12f4b86ee854734132c36c40e6e167
https://doi.org/10.1111/j.1365-3083.1991.tb02494.x

Recombinant human serum amyloid P component from Pichia pastoris: production and characterization

Autor: Susanne Boysen, Peter Højrup, S.-E. Svehag, Per Wittenhagen, Jens Jørgen Lønsmann Iversen, Ellen Holm Nielsen, Inger Andersen, Berit Fogh-Schultz

Publikováno v: Boysen, S, Fogh-Schultz, B, Andersen, I, Højrup, P, Iversen, J J L, Wittenhagen, P, Nielsen, E H & Svehag, S-E 2004, ' Recombinant human serum amyloid P component from Pichia pastoris: Production and characterization. ', Protein Expression and Purification, vol. 35, pp. 284-292 . https://doi.org/10.1016/j.pep.2004.01.011

Human serum amyloid P component (SAP) was expressed in the methylotrophic yeast Pichia pastoris . SAP cDNA was placed under control of regulatory sequences derived from the alcohol oxidase gene (AOX1), and its protein product was secreted using the S

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8e51fd13bd6ce788652001214f1761c
https://pubmed.ncbi.nlm.nih.gov/15135404