Zobrazeno 1 - 10
of 25
pro vyhledávání: '"S V, Pan'kov"'
Autor:
O. G. Somova, Alexander V. Chudinov, R. A. Yurasov, O. V. Moiseeva, Dmitry Prokopenko, Olga V. Antonova, Dmitry A. Gryadunov, S. V. Pan'kov, V. R. Chechetkin
Publikováno v:
Journal of Biomolecular Structure and Dynamics. 27:235-244
Among various factors affecting operation of oligonucleotide microchips, the variations in concentration and in homogeneous distribution of immobilized probes over the cells are one of the most important. The labeling of immobilized probes ensures th
Autor:
S. V. Pan'kov, A. Yu. Rubina, D.A. Zubtsov, O. V. Moiseeva, Alexander S. Zasedatelev, E. N. Savvateeva, E. V. Konovalova, V. R. Chechetkin
Publikováno v:
Analytical Biochemistry. 368:205-213
Protein microchips are designed for high-throughput evaluation of the concentrations and activities of various proteins. The rapid advance in microchip technology and a wide variety of existing techniques pose the problem of unified approach to the a
Autor:
M. A. Livshits, M. Y. Donnikov, N. V. Sorokin, S. V. Pan'kov, Alexander S. Zasedatelev, Sergey A. Lapa, Dmitry A. Gryadunov, V. R. Chechetkin
Publikováno v:
Scopus-Elsevier
The efficiency of discrimination between perfect and mismatched duplexes during hybridization on microchips depends on the concentrations of target DNA in solution and immobilized probes, buffer composition, and temperature of hybridization and is de
Autor:
Alexander V. Chudinov, T Ivaschenko, R. A. Urasov, Andrey S. Glotov, Sergey Surzhikov, Alexander S. Zasedatelev, Tatyana V. Nasedkina, S. V. Pan'kov, Vladislav S Baranov
Publikováno v:
Molecular Biology. 39:357-365
Large-scale population studies, diagnosis of genetic predisposition to a broad range of multifactorial diseases, and screening of polymorphic loci associated with individual drug resistance need efficient, accurate, and rapid techniques for identifyi
Autor:
A. S. Krylov, A. D. Mirzabekov, A. Yu. Rubina, S. V. Pan'kov, Alexander V. Chudinov, A. L. Mikheikin, Alexander S. Zasedatelev, A. I. Yaroshchuk
Publikováno v:
Molecular Biology. 37:902-911
To assess the DNA amount in samples (e.g., in biological microchip gel pads) by means of fluorescent dyes, one should use the dyes whose fluorescence weakly depends on DNA composition and structure. With the ImD-310 dye created for this purpose, we h
Autor:
Andrei D. Mirzabekov, E L Darii, A. A. Stomakhin, V. E. Barsky, S M Ivanov, E V Konovalova, S V Pan'kov, A. Yu. Rubina, Ekaterina Dementieva
Publikováno v:
Scopus-Elsevier
Here a simple, reproducible, and versatile method is described for manufacturing protein and ligand chips. The photo-induced copolymerization of acrylamide-based gel monomers with different probes (oligonucleotides, DNA, proteins, and low-molecular l
Publikováno v:
Russian Journal of Organic Chemistry. 37:426-429
Phenols with pKa ≥ 7 react with 1-cyanoimidazole and 1-cyano-2-methylimidazole to give addition products at the cyano group; phenols with pKa ≤ 1 give rise to the corresponding quaternary salts, 1-cyanoimidazolium phenolates; phenols with pKa ≈
Publikováno v:
Russian Journal of General Chemistry. 71:763-766
The hydrolysis kinetics of N-cyanoazoles in alkaline solutions is described by a first-order kinetic equation with respect to the substrate and concentration of hydroxide ions. The hydrolysis rate constants increase with increasing number of nitrogen
Autor:
V E, Barskiĭ, E E, Egorov, É Ia, Kreĭndlin, Iu P, Lysov, S V, Pan'kov, D A, Iurasov, R A, Iurasov, A S, Zasedatelev
Publikováno v:
Biofizika. 57(3)
This paper discusses an issue on the development of biophysical methods for biochip analysis. A scheme and construction of a biochip analyzer based on wide-field digital fluorescence microscopy are described. The analyzer is designed to register imag
Publikováno v:
Russian Journal of General Chemistry. 72:1286-1288
Alkyl imidazole-1-carboximidates and alkyl benzimidazole-1-carboximidates were synthesized by reaction of 1-cyanoimidazole and 1-cyanobenzimidazole with aliphatic alcohols. The reaction time and the product yield depend on the structure of the alcoho