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A new method for the construction of translationally coupled operons in a bacterial chromosome

Autor: Zinaida Polonskaya, A. Yu. Gulevich, S. V. Mashko, Irina Vladimirovna Biryukova, Danila Vadimovich Zimenkov, Alexander A. Krylov, V. Yu. Ermishev, N. I. Minaeva, A. Yu. Skorokhodova

Publikováno v: Molecular Biology. 43:505-514

A new method for the construction of translationally coupled operons in a bacterial chromosome was developed on the basis of the recombineering approach. The method includes the in vitro construction of an artificial operon with an efficiently transl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::71403e80e7cad3981ce047f66d95788d
https://doi.org/10.1134/s0026893309030194

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Tuning the Expression Level of a Gene Located on a Bacterial Chromosome

Autor: N. I. Minaeva, Vera Georgievna Doroshenko, A. Yu. Gulevich, Irina Vladimirovna Biryukova, S. V. Mashko, A. Yu. Skorokhodova, Danila Vadimovich Zimenkov, Joanna I. Katashkina

Publikováno v: Molecular Biology. 39:719-726

A new method of constructing a set of bacterial cell clones varying in the strength of a promoter upstream of the gene of interest was developed with the use of Escherichia coli MG1655 and lacZ as a reporter. The gist of it lies in constructing a set

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cdea6f56e0f3e65c129ab2722c37c12a
https://doi.org/10.1007/s11008-005-0087-8

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[Construction of the new Escherichia coli K-12 wild-type strain with improved growth characteristics for application in metabolic engineering]

Autor: I V, Biriukova, A A, Krylov, E M, Kiseleva, N I, Minaeva, S V, Mashko

Publikováno v: Genetika. 46(3)

MG1655 of Escherichia coli K-12 is frequently used in metabolic engineering as the wild-type strain. However, its two mutations, ilvG and rph-1 provide a negative effect on culture growth. The "polar effect" of rph-1 decreases the level of pyrE expre

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::3ba23ffabce4948f55137680265bda3b
https://pubmed.ncbi.nlm.nih.gov/20391779

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[New method of construction of artificial translational-coupled operons in bacterial chromosome]

Autor: A Iu, Gulevich, A Iu, Skorokhodova, V Iu, Ermishev, A A, Krylov, N I, Minaeva, Z M, Polonskaia, D V, Zimenkov, I V, Biriukova, S V, Mashko

Publikováno v: Molekuliarnaia biologiia. 43(3)

The new method of translational-coupled operons construction in bacterial chromosome has been developed on the basis of recombineering approach. It includes construction in vitro of the artificial operon with efficiently translated proximal cistron f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::315ecb5c0d45eabd89882ac8b0ac4b40
https://pubmed.ncbi.nlm.nih.gov/19548541

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[Gene yddG of Escherichia coli encoding the putative exporter of aromatic amino acids: constitutive transcription and dependence of the expression level on the cell growth rate]

Autor: I S, Tsyrenzhapova, V G, Doroshenko, L G, Aĭrikh, A S, Mironov, S V, Mashko

Publikováno v: Genetika. 45(5)

Gene yddG of Escherichia coli encodes a protein of the inner membrane. Data obtained earlier demonstrated that under conditions of oversynthesis of aromatic amino acids, YddG promotes their export from E. coli cells. In this work, a method of primer

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::2cc6cb4856b5906da861806d460dff07
https://pubmed.ncbi.nlm.nih.gov/19534419

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[Tuning of expression level of the genes of interest located in the bacterial chromosome]

Autor: Zh I, Katashkina, A Iu, Skorokhodova, D V, Zimenkov, A Iu, Gulevich, N I, Minaeva, V G, Doroshenko, I V, Biriukova, S V, Mashko

Publikováno v: Molekuliarnaia biologiia. 39(5)

The new method of construction of the set of E. coli clones, differing in the promoter strength upstream the gene of interest, has been developed and tested using native E. coli MG 1655 lacZ gene as the reporter. This method includes the construction

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::518317f02d1a73ed3ef97e4591bfa10c
https://pubmed.ncbi.nlm.nih.gov/16240716

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[Decrease in the level of DeoR-dependent repression of the deo operon as a result of integration of foreign DNA fragments into the interoperator deoO1-deoO2 region of the Escherichia coli chromosome]

Autor: N A, Mochul'skaia, A S, Mironov, S V, Mashko

Publikováno v: Genetika. 30(9)

Two heterologous DNA fragments encoding the genes Kanr and cat were integrated into the regulatory region of the deo operon of Escherichia coli, located in a recombinant plasmid. As a result, plasmids pD5K and pD5C were obtained, in which the distanc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::2688f3ab9387b7a551144c741e739fa9
https://pubmed.ncbi.nlm.nih.gov/8001800

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