Zobrazeno 1 - 8
of 8
pro vyhledávání: '"S Sh, Gapizov"'
Autor:
D. A. Dolgikh, L. N. Shingarova, Alexander V. Zlobinov, Rustam H. Ziganshin, Vasiliy A. Mironov, S. Sh. Gapizov, Lada E. Petrovskaya, Mikhail P. Kirpichnikov, G.Y. Lomakina, E. A. Kryukova
Publikováno v:
Applied biochemistry and biotechnology. 193(11)
Overproduction of the membrane proteins in Escherichia coli cells is a common approach to obtain sufficient material for their functional and structural studies. However, the efficiency of this process can be limited by toxic effects which decrease t
Autor:
Lada E. Petrovskaya, Dmitry A. Dolgikh, S. Sh. Gapizov, Shingarova Ln, E. V. Svirschevskaya, Mikhail P. Kirpichnikov
Publikováno v:
Acta Naturae. 10:34-42
Endothelial cells play a major role in the development of inflammation and neoangiogenesis in cancer and chronic inflammatory diseases. In 3D cultures, cells are under conditions that closely resemble those existing in healthy and disease-stricken hu
Autor:
E. A. Kryukova, S. Sh. Gapizov, M.V. Kryukova, Elizaveta Rivkina, Mikhail P. Kirpichnikov, Dmitry A. Dolgikh, Yana Y. Berlina, Lada E. Petrovskaya, L. N. Shingarova
Publikováno v:
Biomolecules, Vol 11, Iss 1229, p 1229 (2021)
Biomolecules
Volume 11
Issue 8
Biomolecules
Volume 11
Issue 8
A gene coding for a novel putative amylase, oligo-1,6-glucosidase from a psychrotrophic bacterium Exiguobacterium sibiricum from Siberian permafrost soil was cloned and expressed in Escherichia coli. The amino acid sequence of the predicted protein E
Autor:
Lada E. Petrovskaya, K R Birikh, E. A. Kryukova, Dmitry A. Dolgikh, S.A. Yakimov, A. V. Zlobinov, Mikhail P. Kirpichnikov, Shingarova Ln, E. F. Boldyreva, S. Sh. Gapizov
Publikováno v:
Biochemistry. Biokhimiia. 83(6)
Construction of antibody mimetics on the base of alternative scaffold proteins is a promising strategy for obtaining new products for medicine and biotechnology. The aim of our work was to optimize the cell display system for the 10th human fibronect
Autor:
S. A. Yakimov, S. Sh. Gapizov, D. A. Dolgikh, E. A. Kryukova, Lada E. Petrovskaya, Mikhail P. Kirpichnikov, E. V. Svirshchevskaya, E. P. Lukashev, E. F. Boldyreva, L. N. Shingarova
Publikováno v:
Biotechnology and applied biochemistryReferences. 66(4)
Fusion with an albumin-binding domain (ABD) of streptococcal protein G represents a popular approach for half-life extension of small protein therapeutics in the organism. To increase the circulation time of engineered αvβ3-integrin-binding protein
Autor:
S. Sh. Gapizov, K. A. Novototskaya-Vlasova, Elizaveta Rivkina, A. V. Zlobinov, Dmitry A. Dolgikh, Mikhail P. Kirpichnikov, Shingarova Ln, E. F. Boldyreva, Lada E. Petrovskaya
Publikováno v:
Extremophiles : life under extreme conditions. 22(1)
Cell surface display is a popular approach for the construction of whole-cell biocatalysts, live vaccines, and screening of combinatorial libraries. To develop a novel surface display system for the popular scaffold protein 10th human fibronectin typ
Autor:
Evgeny P. Lukashev, Mikhail P. Kirpichnikov, E. A. Kryukova, S. Sh. Gapizov, Dmitry A. Dolgikh, S.A. Yakimov, Shingarova Ln, E. F. Boldyreva, E. V. Svirschevskaya, Lada E. Petrovskaya
Publikováno v:
Russian Journal of Bioorganic Chemistry. 40:375-382
Hybrid molecules of a new type bearing a red fluorescent protein mCherry and one of the alternative scaffold proteins, the 10th human fibronectin type III domain (10Fn3), which can be used for the construction of antibody mimics with various binding
Autor:
L E, Petrovskaia, S Sh, Gapizov, L N, Shingarova, E A, Kriukova, E F, Boldyreva, S A, Iakimov, E V, Svirshchevskaia, E P, Lukashev, D A, Dolgikh, M P, Kirpichnikov
Publikováno v:
Bioorganicheskaia khimiia. 40(4)
In the current paper we describe a new type of hybrid molecules including red fluorescent protein mCherry and 10th type III human fibronectin domain (10Fn3) - one of the alternative scaffold proteins which can be used for the construction of antibody