Výsledky vyhledávání - "S M Loosmore"

Identification of T- and B-cell epitopes of the S2 and S3 subunits of pertussis toxin by use of synthetic peptides

Autor: C Sia, G. Zobrist, S M Loosmore, Pele Chong, Michèl R. Klein

Publikováno v: Infection and Immunity. 60:4640-4647

To design an optimized synthetic vaccine against whooping cough, we have studied the biological and immunological properties of three peptides of the S2 subunit and nine overlapping synthetic peptides covering the entire sequence of the S3 subunit of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21652444be70f8fd403caaaa7572df67
https://doi.org/10.1128/iai.60.11.4640-4647.1992

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Engineering of genetically detoxified pertussis toxin analogs for development of a recombinant whooping cough vaccine

Autor: G R Zealey, Pele Chong, R E Fahim, F L Yao, K Radika, R K Yacoob, S M Loosmore, G. Zobrist, Stephen A. Cockle, Heather A. Boux

Publikováno v: Infection and Immunity. 58:3653-3662

Pertussis toxin (PT) is an important protective antigen in vaccines against whooping cough, and a genetically detoxified PT analog is the preferred form of the immunogen. Several amino acids of the S1 subunit were identified as functionally critical

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dcc13dab6548073712f4905ee8febba8
https://doi.org/10.1128/iai.58.11.3653-3662.1990

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Properties of recombinant HtrA: an otitis media vaccine candidate antigen from non-typeable Haemophilus influenzae

Autor: G A, Cates, Y P, Yang, V, Klyushnichenko, R, Oomen, S M, Loosmore

Publikováno v: Developments in biologicals. 103

Non-encapsulated or non-typable Haemophilus influenzae (NTHi) is a major cause of middle ear infections in young children. HtrA has been identified as a vaccine candidate antigen from NTHi; therefore physicochemical characterization of this antigen i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::afce2e1b8efdbc4e8a4b5f022236e265
https://pubmed.ncbi.nlm.nih.gov/11214237

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Characterization of Moraxella (Branhamella) catarrhalis lbpB, lbpA, and lactoferrin receptor orf3 isogenic mutants

Autor: R A, Bonnah, H, Wong, S M, Loosmore, A B, Schryvers

Publikováno v: Infection and immunity. 67(3)

Pathogenic members of the family Neisseriaceae produce specific receptors to acquire iron from their host’s lactoferrin and transferrin. Recently, putative Moraxella catarrhalis lactoferrin receptor genes and a third open reading frame (lbpB, lbpA,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d10ac96a42c81062eadb4bb2e99fe4e2
https://pubmed.ncbi.nlm.nih.gov/10024604

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Characterization of pertussis toxin analogs containing mutations in B-oligomer subunits

Autor: Stephen A. Cockle, G R Zealey, Pele Chong, R K Yacoob, M. Klein, Heather A. Boux, S M Loosmore

Publikováno v: Infection and immunity. 61(6)

The S2, S3, and S4 subunit genes of pertussis toxin (PT) from Bordetella pertussis were subjected to site-directed mutagenesis, and the resultant PT analogs were assayed for altered biological properties. PT analogs S2(T91,R92,N93) delta and S2(Y102A

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bba7f5a46f67c81ebb0e3e73b8db8b4
https://pubmed.ncbi.nlm.nih.gov/8500874

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Construction of Bordetella pertussis strains that overproduce genetically inactivated pertussis toxin

Autor: A B Herbert, R K Yacoob, N J Mackay, Michel Klein, Stephen A. Cockle, S M Loosmore, G R Zealey, L D Miller

Publikováno v: Applied and environmental microbiology. 58(1)

Nontoxic analogs of pertussis toxin (PT), produced by in vitro mutagenesis of the tox operon, are immunogenic and protective against infection by Bordetella pertussis. The moderate levels of PT production by B. pertussis, however, make it the limitin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::edce48402717fbec4775f46b5f79c467
https://pubmed.ncbi.nlm.nih.gov/1539974

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Gene replacement in Bordetella pertussis by transformation with linear DNA

Autor: L J Boux, S M Loosmore, L D Miller, Stephen A. Cockle, G R Zealey, R K Yacoob, Michel Klein

Publikováno v: Bio/technology (Nature Publishing Company). 8(11)

We replaced the wild-type TOX operon of Bordetella pertussis with in vitro mutated, detoxified alleles by electroporetic transformation using unmarked linear DNA. Uptake of DNA was selected by transient ampicillin resistance and two simultaneous reco

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::548bd4966ac133a648b73374cd5a18b8
https://pubmed.ncbi.nlm.nih.gov/1366929

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