Zobrazeno 1 - 10
of 45
pro vyhledávání: '"S M, Schuster"'
Autor:
C M, Westhoff, O, Lopez, P, Goebel, L, Carlson, R R, Carlson, F W, Wagner, S M, Schuster, D E, Wylie
Publikováno v:
Proteins. 37(3)
Monoclonal antibodies (mAb) specific for mercuric ions were isolated from BALB/c mice injected with a mercury-containing, hapten-carrier complex. The antibodies reacted by enzyme-linked immunosorbent assay with bovine serum albumin-glutathione-mercur
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8c545199cad666005c2483e8518c4d73
https://pubmed.ncbi.nlm.nih.gov/10591102
https://pubmed.ncbi.nlm.nih.gov/10591102
Publikováno v:
The Biochemical journal. 339
Transcription of the asparagine synthetase (AS) gene is induced by amino acid deprivation. The present data illustrate that this gene is also under transcriptional control by carbohydrate availability. Incubation of human HepG2 hepatoma cells in gluc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::0c829346923383210ea3f809c7f8fe1f
https://pubmed.ncbi.nlm.nih.gov/10085239
https://pubmed.ncbi.nlm.nih.gov/10085239
Autor:
N G, Richards, S M, Schuster
Publikováno v:
Advances in enzymology and related areas of molecular biology. 72
The enzymatic synthesis of asparagine is an ATP-dependent process that utilizes the nitrogen atom derived from either glutamine or ammonia. Despite a long history of kinetic and mechanistic investigation, there is no universally accepted catalytic me
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::ebbd8f0521fc46d721050c0980a2ff8c
https://pubmed.ncbi.nlm.nih.gov/9559053
https://pubmed.ncbi.nlm.nih.gov/9559053
Publikováno v:
The Journal of biological chemistry. 269(43)
Although Arg-30, Asn-74, and Asn-79 appear totally conserved throughout the purF glutamine-dependent amidotransferases, their potential roles in catalysis and binding remain unexplored for any member of the enzyme family. Here we report the overexpre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::44ab24f08022b3e4ca21ddb297749945
https://pubmed.ncbi.nlm.nih.gov/7929415
https://pubmed.ncbi.nlm.nih.gov/7929415
Publikováno v:
The Journal of biological chemistry. 269(10)
The mechanism of nitrogen transfer in glutamine-dependent amidotransferases remains to be unambiguously established. We now report the overexpression, purification, and kinetic characterization of both the glutamine- and ammonia-dependent activities
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::afed63fa19cbe101faeeb5106e7b538f
https://pubmed.ncbi.nlm.nih.gov/7907328
https://pubmed.ncbi.nlm.nih.gov/7907328
Publikováno v:
The Journal of biological chemistry. 268(22)
Cys-1 mutants of recombinant human asparagine synthetase were constructed and their ability to catalyze the glutamine-dependent nitrogen transfer reaction required for asparagine biosynthesis was determined. In agreement with previous work, altering
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::78368c1596306445c9fea41d76f3f419
https://pubmed.ncbi.nlm.nih.gov/8102140
https://pubmed.ncbi.nlm.nih.gov/8102140
Autor:
S, Sheng, S M, Schuster
Publikováno v:
The Journal of biological chemistry. 268(7)
A yeast protein was purified from wild type Saccharomyces cerevisiae (S. cerevisiae) to near homogeneity using an ethanolamine affinity chromatography procedure. The N-terminal amino acid sequencing and the amino acid composition analyses identified
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6eaa2e68fc72ed8cd45b658dda5f61a8
https://pubmed.ncbi.nlm.nih.gov/8444852
https://pubmed.ncbi.nlm.nih.gov/8444852
Publikováno v:
The Journal of biological chemistry. 268(2)
The resistance of certain tumor cells to the chemotherapeutic agent L-asparaginase has often been found to be associated with the presence of asparagine synthetase activity. In an attempt to study the translational regulation of the asparagine synthe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::bdaed956fa0cd535b205a3173d57b49e
https://pubmed.ncbi.nlm.nih.gov/8093451
https://pubmed.ncbi.nlm.nih.gov/8093451
Autor:
S, Sheng, S M, Schuster
Publikováno v:
BioTechniques. 13(5)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6a635a737b6e95b0e96a61e4a2365b26
https://pubmed.ncbi.nlm.nih.gov/1418968
https://pubmed.ncbi.nlm.nih.gov/1418968
Publikováno v:
The Journal of biological chemistry. 267(1)
A highly conserved protein motif characteristic of Class II aminoacyl tRNA synthetases was found to align with a region of Escherichia coli asparagine synthetase A. The alignment was most striking for aspartyl tRNA synthetase, an enzyme with catalyti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::a67def418fdc089e98595c0b7e12af91
https://pubmed.ncbi.nlm.nih.gov/1346128
https://pubmed.ncbi.nlm.nih.gov/1346128