Zobrazeno 1 - 10
of 58
pro vyhledávání: '"S M, Avaeva"'
Autor:
Ju P, Vainonen, N N, Vorobyeva, E V, Rodina, T I, Nazarova, S A, Kurilova, Ju S, Skoblov, S M, Avaeva
Publikováno v:
Biochemistry. Biokhimiia. 70(1)
Soluble inorganic pyrophosphatase from Escherichia coli (E-PPase) is a hexamer forming under acidic conditions the active trimers. We have earlier found that the hydrolysis of a substrate (MgPP(i)) by the trimers as well as a mutant E-PPase Asp26Ala
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::adecc317698e0626dff329b14d47a191
https://pubmed.ncbi.nlm.nih.gov/15701051
https://pubmed.ncbi.nlm.nih.gov/15701051
Publikováno v:
Bioorganicheskaia khimiia. 28(5)
The conditions were found for obtaining trimeric, dimeric, and monomeric forms of the Escherichia coli inorganic pyrophosphatase from its native hexameric form. Interconversions of the oligomers were studied, and rate constants for their dissociation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::997c8105cbb6475f2b582eb33947c182
https://pubmed.ncbi.nlm.nih.gov/12408026
https://pubmed.ncbi.nlm.nih.gov/12408026
Autor:
V R, Samygina, A N, Popov, E V, Rodina, N N, Vorobyeva, V S, Lamzin, K M, Polyakov, S A, Kurilova, T I, Nazarova, S M, Avaeva
Publikováno v:
Journal of molecular biology. 314(3)
Two structures of Escherichia coli soluble inorganic pyrophosphatase (EPPase) complexed with calcium pyrophosphate (CaPP(i)-EPPase) and with Ca(2+) (Ca(2+)-EPPase) have been solved at 1.2 and 1.1 A resolution, respectively. In the presence of Mg(2+),
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::b5b30049b61bf11d12ca72b79d628f50
https://pubmed.ncbi.nlm.nih.gov/11846572
https://pubmed.ncbi.nlm.nih.gov/11846572
Publikováno v:
European journal of biochemistry. 268(13)
Excess of Mg2+ ions is known to inhibit the soluble inorganic pyrophosphatases (PPases). In contrast, the mutant Escherichia coli inorganic pyrophosphatase Asp42--Asn is three times more active than native and retains its activity at high Mg2+ concen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::cbe93451c268f4cf9b8d050acd520c87
https://pubmed.ncbi.nlm.nih.gov/11432753
https://pubmed.ncbi.nlm.nih.gov/11432753
Publikováno v:
Bioorganicheskaia khimiia. 27(1)
The interaction of inorganic pyrophosphatase from E. coli with inorganic phosphate (Pi) was studied in a wide concentration range of phosphate. The apoenzyme gives two inactive compounds with Pi, a product of phosphorylation of the carboxylic group o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::bdcca9cbfc65ba6aa0c5f0da8af2ed9c
https://pubmed.ncbi.nlm.nih.gov/11255639
https://pubmed.ncbi.nlm.nih.gov/11255639
Autor:
S M, Avaeva
Publikováno v:
Biochemistry. Biokhimiia. 65(3)
Recent progress in studies of the mode of action of cytoplasmic inorganic pyrophosphatases is mainly due to the analysis of a dozen and a half structures of the apoenzyme, its complexes, and mutants. However, despite considerable research on the mech
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::3b185e8025f8bfe18d4a810ee382a55f
https://pubmed.ncbi.nlm.nih.gov/10739480
https://pubmed.ncbi.nlm.nih.gov/10739480
Publikováno v:
Biochemistry. Biokhimiia. 64(2)
Magnesium-supported PPi hydrolysis by the mutant Asp-67Asn E. coli pyrophosphatase at saturating PPi and metal-activator concentrations in the presence of NaF is followed by a gradual decrease in the initial rate of PPi hydrolysis. The reaction occur
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::b4fe7737ea5a05e49546085311ccb971
https://pubmed.ncbi.nlm.nih.gov/10187907
https://pubmed.ncbi.nlm.nih.gov/10187907
Autor:
S M, Avaeva, E V, Rodina, N N, Vorobyeva, S A, Kurilova, T I, Nazarova, V A, Sklyankina, V Y, Oganessyan, V R, Samygina, E H, Harutyunyan
Publikováno v:
Biochemistry. Biokhimiia. 63(6)
The three-dimensional structures of four mutant E. coli inorganic pyrophosphatases (PPases) with single Asp--Asn substitutions at positions 42, 65, 70, and 97 were solved at 1.95, 2.15, 2.10, and 2.20 A resolution, respectively. Asp-42--Asn and Asp-6
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::de80f67f6cbe295f8bccb4a66e178594
https://pubmed.ncbi.nlm.nih.gov/9668207
https://pubmed.ncbi.nlm.nih.gov/9668207
Autor:
S M, Avaeva, E V, Rodina, N N, Vorobyeva, S A, Kurilova, T I, Nazarova, V A, Sklyankina, V Y, Oganessyan, E H, Harutyunyan
Publikováno v:
Biochemistry. Biokhimiia. 63(5)
The three-dimensional structures of E. coli inorganic pyrophosphatase (PPase) and its complexes with Mn2+ in a high affinity site and with Mg2+ in high and low affinity sites determined by authors in 1994-1996 at 1.9-2.2 A resolution are compared. Me
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::5a190cea49622c979be5b5afba64e680
https://pubmed.ncbi.nlm.nih.gov/9632898
https://pubmed.ncbi.nlm.nih.gov/9632898
Publikováno v:
Bioorganicheskaia khimiia. 23(2)
An unusually high reactivity of the carboxyl groups of the active site of E. coli inorganic pyrophosphatase towards amines was shown. Amino acid esters and other amines are specific irreversible inhibitors of the enzyme. The reaction involves the for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::517a88f838a55facdb3a2ccdc34419cb
https://pubmed.ncbi.nlm.nih.gov/9157843
https://pubmed.ncbi.nlm.nih.gov/9157843