Zobrazeno 1 - 2
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pro vyhledávání: '"S K, Boehlein"'
Publikováno v:
The Journal of biological chemistry. 269(43)
Although Arg-30, Asn-74, and Asn-79 appear totally conserved throughout the purF glutamine-dependent amidotransferases, their potential roles in catalysis and binding remain unexplored for any member of the enzyme family. Here we report the overexpre
Publikováno v:
The Journal of biological chemistry. 269(10)
The mechanism of nitrogen transfer in glutamine-dependent amidotransferases remains to be unambiguously established. We now report the overexpression, purification, and kinetic characterization of both the glutamine- and ammonia-dependent activities