Výsledky vyhledávání - "S Ia, Ditiatkin"

[Glycine-dependent cryotransformation of Bacillus anthracis by DNA of various plasmids]

Autor: A S, Stepanov, O B, Puzanova, Iu V, Brandzishevskiĭ, O G, Loginova, S Ia, Ditiatkin, B I, Il'iashenko

Publikováno v: Molekuliarnaia genetika, mikrobiologiia i virusologiia. (3)

The possibility of the 1,8-60,0 Md plasmids DNA transfer into the cells of different Bacillus anthracis strains has been established. A number of parameters of the procedure has been optimized. The including of PEG6000 into the cryomixture in the fin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::fbeb95053b21f9316c7acbdbef24a4fa
https://pubmed.ncbi.nlm.nih.gov/1906984

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[The genetic transfer, heredity and phenotypic expression of plasmid RP4::Mu cts genes in Bacillus cereus]

Autor: N V, Timakova, G I, Aleshkin, I V, Titova, M G, Goderdzishvili, V I, Zakharenko, G V, Brukhanskiĭ, O G, Loginova, S Ia, Ditiatkin, B N, Il'iashenko

Publikováno v: Biulleten' eksperimental'noi biologii i meditsiny. 109(3)

The transcipients were obtained in intrageneric matings of E.coli donor harbouring the plasmid PR4::Mu cts 62 with Bac. cereus GP7 recipient cells with the frequency 10(-9). The transcipient clone Bac. cereus 682 was selected having stably inherited

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d1463decc9d43cb4d109db159f0b36c3
https://pubmed.ncbi.nlm.nih.gov/2114185

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[Reaction between isolated lambda phage DNA and membrane structures of Escherichia coli cells]

Autor: T F, Moiseeva, S Ia, Ditiatkin, A A, Kim, B N, Il'iashenko

Publikováno v: Biulleten' eksperimental'noi biologii i meditsiny. 89(6)

The saccharose density gradient (30--55%) centrifugation technique applied to E. coli membrane preparations was used to show that treatment of the bacteria with Ca2+ in the cold results in the redistribution of the absorbed phage DNA from the cell wa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1fc018bdb174b7b7533dad32793f67cf
https://pubmed.ncbi.nlm.nih.gov/6446950

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[Cryotransformation and cryotransfection of bacteria]

Autor: B N, Il'iashenko, S Ia, Ditiatkin, I V, Timakova

Publikováno v: Vestnik Akademii meditsinskikh nauk SSSR. (10)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::83322bcf313f493e51466a3db0c92ef4
https://pubmed.ncbi.nlm.nih.gov/3909682

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[Use of transformation for studying the nature of formation of stable bacterial L forms]

Autor: I S, Tartakovskiĭ, S V, Prozorovskiĭ, S Ia, Ditiatkin, L E, Glazacheva

Publikováno v: Zhurnal mikrobiologii, epidemiologii i immunobiologii. (2)

The authors obtained a stable variant of the L-forms of Bacillus subtilis capable of exponential growth of the minimal and synthetic medium. An electron-microscopic study of different stages of the L-form formation was carried out by the method of ul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::bac95a5b94a6fc1b6c4fe72cf41439d7
https://pubmed.ncbi.nlm.nih.gov/404804

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[Glycine-dependent cryotransformation of Bacillus cereus by plasmid DNA]

Autor: O G, Loginova, S Ia, Ditiatkin

Publikováno v: Molekuliarnaia genetika, mikrobiologiia i virusologiia. (11)

A new technique for transformation of naturally noncompetent strains of Bac. cereus is proposed. Penetration of the DNA into recipient cells is based on two-step effect. At the first step of the process bacilli are affected by glycine in the early lo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::52210ab7d9d939e5b92429367a740cf0
https://pubmed.ncbi.nlm.nih.gov/3148860

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[Acceptor capacity of frozen and thawed bacteria in relation to isolated plasmid DNA]

Autor: S Ia, Ditiatkin, B N, Il'iashenko

Publikováno v: Zhurnal mikrobiologii, epidemiologii i immunobiologii. (9)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::67f5a5eabeea3d96626ba37323f3d059
https://pubmed.ncbi.nlm.nih.gov/371299

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[Adsorption of lambda phage DNA onto Escherichia coli cells treated with Ca2+ ions and onto frozen--thawed bacteria]

Autor: T F, Moiseeva, S Ia, Ditiatkin, A A, Kim, B N, Il'iashenko

Publikováno v: Biulleten' eksperimental'noi biologii i meditsiny. 89(3)

The study of the biologically active tritium-labeled phage lambda DNA adosrption on Ca2+-treated and frozen--thawed E. coli cells showed the absence of a correlation between the adsorption level and transfection efficiency. Thus the infectious phage

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::dae2e1c5337842962004fc852425d1c5
https://pubmed.ncbi.nlm.nih.gov/6446330

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[In vivo restriction of Escherichia coli-transforming DNA by endonuclease R.M.EcoRI]

Autor: G I, Aleshkin, N V, Timakova, S Ia, Ditiatkin, B N, Il'iashenko, A G, Skavronskaia

Publikováno v: Genetika. 17(7)

Transformation of Escherichia coli K-12 for various chromosomal markers was accomplished by using AB1157 recBC+ strain as a recipient. The yield of transformants was reduced 10-fold, as compared with that obtained in JC7623 recBC sbcB recipient. Elim

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::4079745b17740433bafa1d2c8705aca6
https://pubmed.ncbi.nlm.nih.gov/6268481

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