Zobrazeno 1 - 10
of 249
pro vyhledávání: '"S Hattman"'
Publikováno v:
Molekuliarnaia biologiia. 38(5)
The review reflects results of studies on the molecular mechanism of phage T4 Dam DNA-methyltransferase action. The enzyme (T4Dam) catalyzes methyl group transfer from S-adenosyl-l-methionine (AdoMet) to N6-adenine position in the palindromic recogni
Publikováno v:
The Journal of biological chemistry. 276(23)
The momP1 promoter of the bacteriophage Mu mom operon is an example of a weak promoter. It contains a 19-base pair suboptimal spacer between the -35 (ACCACA) and -10 (TAGAAT) hexamers. Escherichia coli RNA polymerase is unable to bind to momP1 on its
Publikováno v:
Journal of bacteriology. 180(12)
Late in its growth cycle, transcription of the phage Mu mom Promoter (Pmom) is activated by the phage gene product, C, a site-specific DNA binding protein. In vitro transcription analyses showed that this activation does not require specific contacts
Publikováno v:
Journal of molecular biology. 247(4)
The bacteriophage Mu Com protein is a small "zinc finger-like" protein that binds a specific site in com-mom operon mRNA and activates translation of the mom open-reading-frame. Com contains six cysteine and five histidine residues that have the pote
Publikováno v:
Nucleic acids research. 21(15)
Comparison of the deduced amino acid sequences of DNA-[N(6)-adenine]-methyltransferases has revealed several conserved regions. All of these enzymes contain a DPPY-motif, or a variant of it. By site-directed mutagenesis of a cloned T4 dam gene, we ha
Publikováno v:
Nucleic Acids Research. 21:4856-4856
Publikováno v:
Molecular and Cellular Biology. 4:985-988
We examined the DNA of Saccharomyces cerevisiae by both HpaII-MspI restriction enzyme digestion and high-performance liquid chromatography analysis for the possible presence of 5-methylcytosine. Both of these methods failed to detect cytosine methyla
Publikováno v:
Molecular and Cellular Biology. 1:600-608
Deoxyribonucleic acid (DNA) of the transcriptionally active macronucleus of Tetrahymena thermophila is methylated at the N6 position of adenine to produce methyladenine (MeAde); approximately 1 in every 125 adenine residues (0.8 mol%) is methylated.
Autor:
S Hattman
Publikováno v:
Journal of Bacteriology. 129:1330-1334
A procedure is described for the partial purification of the deoxyribonucleic acid (DNA)-cytosine methylases controlled by the RII plasmid and by the Escherichia coli mec+ gene. The two enzymes exhibit similar but distinct chromatographic behavior on
Publikováno v:
Molecular and Cellular Biology. 4:599-603
Purified nuclear DNA from two mealybug species was analyzed for its 5-methylcytosine (m5C) content by reversed-phase high-pressure liquid chromatography. We observed that the percent m5C (percentage of cytosines which are methylated) varied between t