Zobrazeno 1 - 10
of 40
pro vyhledávání: '"Séverine Frutiger"'
Autor:
Séverine Frutiger, Alexander Kettner, Mireille Astori, Graham J. Hughes, Mario Roggero, Giampietro Corradin, François Spertini
Publikováno v:
Journal of Allergy and Clinical Immunology. 107:914-920
Background: Characterization of the primary structure of allergens is a prerequisite for the design of new diagnostic and therapeutic tools for allergic diseases. Objective: The purpose of this study was the identification and characterization of a l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::887058daeae8f70b3cb0eb417858e072
https://doi.org/10.1067/mai.2001.113867
https://doi.org/10.1067/mai.2001.113867
Autor:
Ulrich Burger, Kamel Besseghir, K. Schwach-Abdellaoui, Damien Jeannerat, Robert Gurny, Alexandra Rothen-Weinhold, Séverine Frutiger-Hughes, Nathalie Oudry, Graham J. Hughes
Publikováno v:
European Journal of Pharmaceutics and Biopharmaceutics, Vol. 49, No 3 (2000) pp. 253-257
Most peptides are susceptible, in vivo, to proteolytic degradation, and it is difficult to formulate and to deliver them without loss of biological activity. In addition, it is often desirable to release them continuously and at a controlled rate ove
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd717cb67d61027fb3abce34e47d48f6
https://doi.org/10.1016/s0939-6411(00)00066-7
https://doi.org/10.1016/s0939-6411(00)00066-7
Autor:
C. Lesieur, Franc Pattus, F. Gisou van der Goot, Séverine Frutiger, Graham J. Hughes, Roland Kellner
Publikováno v:
Journal of Biological Chemistry. 274:36722-36728
Aerolysin is a bacterial pore-forming toxin that is secreted as an inactive precursor, which is then processed at its COOH terminus and finally forms a circular heptameric ring which inserts into membranes to form a pore. We have analyzed the stabili
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a257cf15d42e8a806d65fb9d5af34778
https://doi.org/10.1074/jbc.274.51.36722
https://doi.org/10.1074/jbc.274.51.36722
Autor:
Luc-Alain Savoy, Jean-Claude Jaton, Andrew J. Reason, Séverine Frutiger-Hughes, Graham J. Hughes
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1434:86-93
Human secretory component has seven putative sites for N -linked glycosylation. From tryptic and Glu-C digests we have isolated peptides encompassing asparagines 65, 72, 117, 168, 403, 451 and 481. Analysis by on line HPLC-electrospray mass spectrome
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf487543e1c6e040de102ba9bfb6439a
https://doi.org/10.1016/s0167-4838(99)00168-5
https://doi.org/10.1016/s0167-4838(99)00168-5
Autor:
Claudine Rey-Berthod, Martin Fuhrmann, Bruno Lehner, Séverine Frutiger-Hughes, Nicholas M. Shaw, Andrew Binieda, Graham R. V. Hughes
Publikováno v:
Biochemical Journal. 340:793-801
A pimeloyl-CoA synthetase from Pseudomonas mendocina 35 was purified and characterized, the DNA sequence determined, and the gene cloned into Escherichia coli to yield an active enzyme. The purified enzyme had a pH optimum of approximately 8.0, Km va
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::662fe7fcf404bfc57c743200b1f033c7
https://doi.org/10.1042/bj3400793
https://doi.org/10.1042/bj3400793
Autor:
Denis F. Hochstrasser, Séverine Frutiger, Luca Bini, Jean-Charles Sanchez, R. Raggiaschi, Vitaliano Pallini, Sabrina Liberatori, Claudio De Felice, Graham J. Hughes, Barbara Magi, Rodolfo Bracci, Marc R. Wilkins, Barbara Marzocchi
Publikováno v:
Electrophoresis. 18:2816-2822
Using updated technical procedures (immobilized pH gradients for isoelectric focusing followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis: IPG/SDS-PAGE) we provide a two-dimensional (2-D) map of amniotic fluid (AF) proteins. This ma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4fdddc1864f722c7cc056c6a18d880b
https://doi.org/10.1002/elps.1150181517
https://doi.org/10.1002/elps.1150181517
Autor:
Graham J. Hughes, Jean-Charles Sanchez, Barbara Magi, Sergio Tripodi, Denis F. Hochstrasser, Séverine Frutiger, Piero Tosi, Vitaliano Pallini, Felice Arcuri, Barbara Marzocchi, Marcella Cintorino, Luca Bini
Publikováno v:
Electrophoresis. 18:2832-2841
Reference two-dimensional (2-D) gels are presented for human breast ductal carcinoma and histologically normal tissue. Whole biopsy fragments were analyzed, including epithelial and nonepithelial components. Thirty-five spots have been assigned by ge
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::713c3fda22e6506c0a817e4143288485
https://doi.org/10.1002/elps.1150181519
https://doi.org/10.1002/elps.1150181519
Autor:
Dominique Schaller, Denis F. Hochstrasser, Séverine Frutiger, Jean-Charles Sanchez, Ron D. Appel, Graham J. Hughes, Olivier Georges Golaz, Amos Marc Bairoch
Publikováno v:
Electrophoresis, Vol. 17, No 3 (1996) pp. 556-65
The systematic sequencing of the yeast genome will soon be completed. A new challenge has been launched by the EUROFAN (European Functional Analysis) project whose goal is to elucidate the physiological and biochemical function of newly discovered op
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a6b1b45ccf4be81714cd2ffa66a9e073
https://doi.org/10.1002/elps.1150170326
https://doi.org/10.1002/elps.1150170326
Autor:
Jürg A. Schifferli, Jean-Daniel Tissot, Christian Pasquali, Graham J. Hughes, François Clément, François Spertini, P. Schneider, Nicole Paquet, Denis F. Hochstrasser, Séverine Frutiger
Publikováno v:
Journal of Immunological Methods. 173:63-75
The clonality of immunoglobulins (Igs) in cryoprecipitates (n = 41) was studied by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Our series included 24 cryoglobulins characterized by immunofixation electrophoresis (IF), 12 'trace amo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e17320e1c394f0085ae3bd87019b55b7
https://doi.org/10.1016/0022-1759(94)90284-4
https://doi.org/10.1016/0022-1759(94)90284-4
Autor:
Werner Staudenmann, Denis Banville, Séverine Frutiger, Graham J. Hughes, Markus Müntener, Ursula G. Föhr, Claus W. Heizmann, Beat W. Schäfer, Barbara R. Weber
Publikováno v:
European Journal of Biochemistry. 215:719-727
alpha and beta parvalbumins are Ca(2+)-binding proteins of the EF-hand type. We determined the protein sequence of human brain alpha parvalbumin by mass spectrometry and cloned human beta parvalbumin (or oncomodulin) from genomic DNA and preterm plac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f575b3b07676689c9e9df5c3d7103fbb
https://doi.org/10.1111/j.1432-1033.1993.tb18084.x
https://doi.org/10.1111/j.1432-1033.1993.tb18084.x