Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Sébastien Ferrandon"'
Publikováno v:
Protein Science. 17:466-472
X-ray crystallography is currently the most successful method for determining the three-dimensional structure of membrane proteins. Nevertheless, growing the crystals required for this technique presents one of the major bottlenecks in this area of s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3fe871737ac46dbeb49f1adf783d9118
https://doi.org/10.1110/ps.073263108
https://doi.org/10.1110/ps.073263108
Publikováno v:
Journal of Molecular Biology. 353:1093-1105
The first naturally occurring split intein was found in the dnaE gene of Synechocystis sp. PCC6803 and belongs to a subclass of inteins without a penultimate histidine residue. We describe two high-resolution crystal structures, one derived from an e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7460bd2730b911a44be43f044f66c8f4
https://doi.org/10.1016/j.jmb.2005.09.039
https://doi.org/10.1016/j.jmb.2005.09.039
Autor:
Ming-Qun Xu, Guillaume Lesage, Yi Ding, Zihe Rao, Inca Ghosh, Xuehui Chen, Sébastien Ferrandon
Publikováno v:
Journal of Biological Chemistry. 278:39133-39142
We have determined the crystal structure of a 154-residue intein derived from the dnaB gene of Synechocystis sp. strain PCC6803 and refined it to a 2.0-A resolution. The x-ray structure suggests that this intein possesses two catalytic sites that app
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1847e3cedf474c5c93a51fa8c17223f0
https://doi.org/10.1074/jbc.m306197200
https://doi.org/10.1074/jbc.m306197200
Publikováno v:
Acta Crystallographica Section D Biological Crystallography. 58:1204-1206
A naturally occurring split intein from the dnaE gene of Synechocystis sp. PCC6803 (Ssp DnaE intein) has been purified and crystallized using PEG 8K as precipitant. The crystal belongs to space group P3(1)21 or P3(2)21, with unit-cell parameters a =
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b817e2aaa071905ec442018bdcdd5547
https://doi.org/10.1107/s0907444902006571
https://doi.org/10.1107/s0907444902006571
Publikováno v:
Biochimica et biophysica acta. 1621(1)
Site-directed mutagenesis was carried out to investigate the roles of a number of highly conserved residues of the chitin-binding domain (ChBD) of Bacillus circulans chitinase A1 (ChiA1) in the binding of chitin. Analysis of single alanine replacemen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8182301eab584156db753138126331b5
https://pubmed.ncbi.nlm.nih.gov/12667608
https://pubmed.ncbi.nlm.nih.gov/12667608
Publikováno v:
Acta crystallographica. Section D, Biological crystallography. 58(Pt 7)
A 154-residue mini-intein from the dnaB gene of Synechocystis sp. PCC 6803 (Ssp DnaB intein) has been purified and crystallized using PEG 4000 as a precipitant. The crystal belongs to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b =
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1d279c94e955525a6e2d7adcd6a4b7c
https://pubmed.ncbi.nlm.nih.gov/12077443
https://pubmed.ncbi.nlm.nih.gov/12077443