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Comparison of Different Immunoenzymatic Methods for the Determination of the Fine Specificity and Affinity Constants of Polyclonal Antibodies Against Pseudopeptide Haptens

Autor: P. Jouin, V. Hanin, S. Fournout, Bernard Pau

Publikováno v: Immunological Investigations. 26:549-559

We evaluated two phosphinopeptides and one phosphonopeptide, which are transition state analogs of a proteolytic reaction, for their ability to generate murine polyclonal antibodies. The specificity of these antisera was determined by indirect and co

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42bcb17c74aeabee003b9b731a10ae11
https://doi.org/10.3109/08820139709088539

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Cytokine mRNA expression in pigs infected with Schistosoma japonicum

Autor: Isabelle P. Oswald, S. Fournout, H O Bøgh, R Barlagne, Charles M. Dozois, Maria Vang Johansen

Publikováno v: Parasitology
Parasitology, Cambridge University Press (CUP), 2001, 122, pp.299-307

The pig is a natural host of Schistosoma japonicum and a useful animal model of human disease. In the present study mRNA levels of Th1 (IFN-gamma) and Th2 (IL-10 and IL-4) cytokines were assessed by RT-PCR within tissues from infected pigs. Twelve Da

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db3546b2b29cd62cf8f0fd9c68c648ed
https://hal.inrae.fr/hal-02671416

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Cloning, chromosomal location, and tissue expression of the gene for pig interleukin-18

Autor: Eric Oswald, J M Fairbrother, S. Fournout, M. Yerle, Charles M. Dozois, Philippe Pinton, Isabelle P. Oswald

Publikováno v: Scopus-Elsevier

Interleukin 18 (IL-18), a recently described cytokine, plays an important role in the cell-mediated immune response, in particular through its ability to induce the production of interferon (IFN)-gamma. We cloned pig IL-18 cDNA from the intestinal ep

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::286ebc43d038500cd4ac58cc63f40c86
https://pubmed.ncbi.nlm.nih.gov/10803849

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Lack of a Role of Cytotoxic Necrotizing Factor 1 Toxin from Escherichia coli in Bacterial Pathogenicity and Host Cytokine Response in Infected Germfree Piglets

Autor: J M Fairbrother, F. Daigle, Eric Oswald, S Y Pérès, Clarisse Desautels, Isabelle P. Oswald, Frédéric Herault, C. Segafredo, M. Odin, S. Fournout, Charles M. Dozois, Joëlle Laffitte

Publikováno v: Infection and Immunity
Infection and Immunity, American Society for Microbiology, 2000, 68 (2), pp.839-847. ⟨10.1128/IAI.68.2.839-847.2000⟩
Infection and Immunity 2 (68), 839-847. (2000)

SomeEscherichia colistrains isolated from intestinal or extraintestinal infections in pigs produce cytotoxic necrotizing factor 1 (CNF1). In order to analyze the role of CNF1 in the pathogenesis of porcine colibacillosis, newborn colostrum-deprived g

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b59d318260ab2efa207d970e3acb6ce0
https://europepmc.org/articles/PMC97213/

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Tumor necrosis factor is required for the priming of peritoneal macrophages by trehalose dimycolate

Autor: I P, Oswald, C M, Dozois, S, Fournout, J F, Petit, G, Lemaire

Publikováno v: European cytokine network. 10(4)

Trehalose dimycolate (TDM), a glycolipid present in the cell wall of Mycobacterium spp., is a powerful immunostimulant. We have developed an original model of macrophage activation where TDM is injected in vivo to prime peritoneal macrophages. These

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::3ebb80cb4f5c408bde09288b3ee9eee7
https://pubmed.ncbi.nlm.nih.gov/10586120

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Development and standardization of an immuno-quantified solid phase assay for HIV-1 aspartyl protease activity and its application to the evaluation of inhibitors

Autor: V. Valverde, P. Jouin, S. Fournout, S. L. Salhi, Bernard Pau, V. Hanin, M. Nicolas, J. M. Masson, Françoise Roquet, R. Seyer

Publikováno v: Analytical chemistry. 69(9)

The catELISA technique was modified and standardized for measuring HIV-1 aspartyl protease activity and evaluating the potency of synthetic peptide inhibitors. This immuno-quantified solid phase assay combines the use of an immobilized C-terminal bio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c24fb2742188bd59b603b3261b0d3f8
https://pubmed.ncbi.nlm.nih.gov/9145028

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