Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Ryoichi Toyoshima"'
Publikováno v:
Journal of Pharmacological Sciences, Vol 125, Iss 2, Pp 157-168 (2014)
Abstract.: The functional activation of Gi/o proteins coupled to muscarinic acetylcholine receptors (mAChRs) was investigated with the conventional guanosine-5′-O-(3-[35S]thio)triphosphate ([35S]GTPγS) binding assay in rat brain membranes. The mos
Externí odkaz:
https://doaj.org/article/27af17f1812c4ddb91dc1a474e9488e4
Autor:
Yuji Odagaki, Ryoichi Toyoshima
Publikováno v:
Journal of Pharmacological Sciences, Vol 98, Iss 1, Pp 66-76 (2005)
5-HT-stimulated [35S]GTPγS binding to rat hippocampal membranes was pharmacologically characterized. Signal/noise ratio or percent increase over basal was optimized with 100 µM GDP, 2 –10 mM MgCl2, and 150 – 200 mM NaCl. However, we preferred t
Externí odkaz:
https://doaj.org/article/bf02088b21ce48729ef078de15de8c78
Publikováno v:
European Journal of Pharmacology. 726:109-115
Functional coupling between serotonin2A (5-HT2A) receptors and Gαq proteins in native brain membranes has been sparsely reported thus far. In the present study, the guanosine-5׳-O-(3-[35S]thio)triphosphate ([35S]GTPγS) binding assay combined with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::10e78f8afae0f09e5d4d673e780a1b77
https://doi.org/10.1016/j.ejphar.2013.12.040
https://doi.org/10.1016/j.ejphar.2013.12.040
Publikováno v:
Naunyn-Schmiedeberg's Archives of Pharmacology. 386:937-947
This study aimed to pharmacologically characterize the response derived from functional activation of Gq proteins coupled with native muscarinic acetylcholine receptors in rat cerebral cortex and hippocampus. Rat cerebral cortical and hippocampal mem
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33b53d838411593f8b1b5d3c1a343e49
https://doi.org/10.1007/s00210-013-0887-7
https://doi.org/10.1007/s00210-013-0887-7
Autor:
Ryoichi Toyoshima, Yuji Odagaki
Publikováno v:
Pharmacology. 92:2-10
Background/Aims: Functional activation of Gq coupled with 5-HT2 receptors was investigated in rat cerebral cortical membranes. Methods: Antibody-capture scintillation proximity assay (SPA)/[35S]GTPγS binding with anti-Gαq antibody was performed. Re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::806c26711d69bbf5cc8c8e4b790adbda
https://doi.org/10.1159/000351745
https://doi.org/10.1159/000351745
Publikováno v:
Basic & Clinical Pharmacology & Toxicology. 109:175-185
Stimulation of specific guanosine-5'-O-(3-[(35)S]thio)triphosphate ([(35)S]GTPγS) binding by l-glutamate was pharmacologically characterized in rat cerebral cortical membranes. Optimization of the experimental conditions with respect to the concentr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0fe0c13490326f9499389497690770da
https://doi.org/10.1111/j.1742-7843.2011.00705.x
https://doi.org/10.1111/j.1742-7843.2011.00705.x
Autor:
Yuji Odagaki, Ryoichi Toyoshima
Publikováno v:
Current Psychiatry Reviews. 6:64-70
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0337016aa5de25d5ad0533b630dd1a62
https://doi.org/10.2174/157340010790596517
https://doi.org/10.2174/157340010790596517
Autor:
Yuji Odagaki, Ryoichi Toyoshima
Publikováno v:
Pharmacological Research. 57:435-444
Using the rat cerebral cortical membranes that had once been frozen and stored at -80 degrees C, the stimulation of specific guanosine-5'-O-(3-[(35)S]thio)triphosphate ([(35)S]GTPgammaS) binding mediated through alpha(2)-adrenergic receptors (alpha(2
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ae0f09fcb0e611dadd489d8c7b934b83
https://doi.org/10.1016/j.phrs.2008.04.006
https://doi.org/10.1016/j.phrs.2008.04.006
Autor:
Ryoichi Toyoshima, Yuji Odagaki
Publikováno v:
Naunyn-Schmiedeberg's Archives of Pharmacology. 372:335-345
Receptor-mediated guanine nucleotide-binding regulatory protein (G protein) activation or functional coupling between receptors and G proteins has been investigated by means of agonist-induced [35S]guanosine-5'-O-(3-thio)triphosphate ([35S]GTPgammaS)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::088933f8af670f5e0b725667d6f9d027
https://doi.org/10.1007/s00210-006-0041-x
https://doi.org/10.1007/s00210-006-0041-x
Autor:
Ryoichi Toyoshima, Yuji Odagaki
Publikováno v:
Journal of Pharmacological Sciences, Vol 98, Iss 1, Pp 66-76 (2005)
5-HT-stimulated [35S]GTPγS binding to rat hippocampal membranes was pharmacologically characterized. Signal/noise ratio or percent increase over basal was optimized with 100 µM GDP, 2 –10 mM MgCl2, and 150 – 200 mM NaCl. However, we preferred t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::78ac7f3ace44946408ea02885537c8d9
https://doi.org/10.1254/jphs.fpj05010x
https://doi.org/10.1254/jphs.fpj05010x