Zobrazeno 1 - 10
of 30
pro vyhledávání: '"Ryo Tomii"'
Autor:
Akio Shirasu, Norihisa Sasayama, Ryo Tomii, Hiroshi Nagashima, Yukiko Handa, Naoko Imai, Buko Ogawa
Publikováno v:
Journal of Reproduction and Development. 57:273-279
The aim of the present study was to optimize the conditions for in vitro development and postvitrification survival of somatic cell cloned feline embryos. To determine the effects of cell cycle synchronization of the nuclear donor cells, we cultured
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::579e52c209c0f3fcd4a5ab1dc9b70655
https://doi.org/10.1262/jrd.10-105a
https://doi.org/10.1262/jrd.10-105a
Autor:
Hiroshi Nagashima, Takashi Ochiai, Koichiro Kano, Naohiro Wako, Mayuko Kurome, Hitomi Matsunari, Ryo Tomii
Publikováno v:
Journal of Reproduction and Development. 55:121-127
Four methods of cell cycle synchronization of porcine preadipocytes for use as nuclear donors in somatic cell cloning were compared: serum starvation, differentiation induction, contact inhibition and roscovitine treatment. After three days of differ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b68618fb1bdadb8e172a0d84e69fdcc7
https://doi.org/10.1262/jrd.20126
https://doi.org/10.1262/jrd.20126
Autor:
Mayuko Kurome, Hajime Yazawa, Takayuki Ishikawa, Arata Shimada, Hiroshi Nagashima, Ryo Tomii, S. Ueno
Publikováno v:
Journal of Reproduction and Development. 54:156-163
Miniature pigs have been recognized as valuable experimental animals in various fields such as medical and pharmaceutical research. However, the amount of information on somatic cell cloning in miniature pigs, as well as genetically modified miniatur
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a7c9075e25c770ba876c907fc73c986
https://doi.org/10.1262/jrd.19165
https://doi.org/10.1262/jrd.19165
Autor:
Shirou Matsumoto, S. Ueno, Ryo Tomii, Hisashi Hisatomi, Mayuko Kurome, Fumio Endo, H. Saito, K. Hiruma, Yuji Kaji, Hiroshi Nagashima, Kimitoshi Nakamura, Mitsuhito Matsumoto, Kenji Okumura
Publikováno v:
Journal of Reproduction and Development. 54:254-258
The aim of the present study was to examine the production efficiency of cloned pigs by serial somatic cell nuclear transfer (SCNT) and to ascertain any changes in the telomere lengths of multiple generations of pigs. Using fetal fibroblasts as the s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83b4c5b00fcb16cfaf8ec64f6a304607
https://doi.org/10.1262/jrd.20038
https://doi.org/10.1262/jrd.20038
Autor:
Hiroshi Nagashima, Hitomi Matsunari, Ryo Tomii, Naoki Nakayama, Mayuko Kurome, H. Saito, K. Hiruma, S. Ueno
Publikováno v:
Biology of Reproduction. 76:900-905
We have successfully produced healthy piglets following cryopreservation of embryos derived from oocytes matured and fertilized in vitro. The appropriate timing of cryopreservation pretreatment (removal of cytoplasmic lipid droplets [delipation] and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::83de73586ff7d0f1fed92f614cfe619a
https://doi.org/10.1095/biolreprod.106.052779
https://doi.org/10.1095/biolreprod.106.052779
Publikováno v:
Journal of Reproduction and Development. 53:1113-1118
The aim of this study was to examine the impact of zona pellucida damage, which might arise during somatic cell nuclear transfer (SCNT), on the development and survival of transferred embryos. The zonae pellucidae of in vitro matured oocytes were eit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62f912817dabd4c1ff454cafa05aeb92
https://doi.org/10.1262/jrd.19063
https://doi.org/10.1262/jrd.19063
Publikováno v:
Journal of Reproduction and Development. 53:1217-1226
Intracytoplasmic sperm injection (ICSI)-mediated gene transfer has recently been shown to be an effective technique for producing transgenic pigs; however, the types of sperm pretreatment having the most beneficial effects on post-ICSI embryogenesis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c94b5d785d845c96e24d62aad1942a8d
https://doi.org/10.1262/jrd.19069
https://doi.org/10.1262/jrd.19069
Publikováno v:
Biochemical and Biophysical Research Communications. 344:455-462
The application of nuclear transfer technology is an interesting approach to investigate stem and progenitor cell transplantation therapy. If stem cells are used as a nuclear donor, donor cells can engraft into cloned animals without histocompatible
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1c4afb8696a70479cf4d6082b711199
https://doi.org/10.1016/j.bbrc.2006.03.148
https://doi.org/10.1016/j.bbrc.2006.03.148
Publikováno v:
Transgenic Research. 15:229-240
The objective of this study was to examine whether the ICSI-mediated gene transfer method using in vitro matured oocytes and frozen sperm head could actually produce transgenic pigs. We also aimed at examining whether transgenic pigs can be cloned fr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::832c44d5dad9607807fbb5977299e5a4
https://doi.org/10.1007/s11248-006-0004-5
https://doi.org/10.1007/s11248-006-0004-5
Publikováno v:
Journal of Reproduction and Development. 51:405-410
Incomplete cytoplasmic maturation of in vitro matured (IVM) oocytes has been known to cause microtubule and microfilament alterations, which may result in abnormal pronuclear formation and failed embryonic development. We examined the influences of m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22f64991cf6ca606ca0c8efa7c3e575d
https://doi.org/10.1262/jrd.16091
https://doi.org/10.1262/jrd.16091