Chemical reversible crosslinking enables measurement of RNA 3D distances and alternative conformations in cells

Autor: Ryan Van Damme, Kongpan Li, Minjie Zhang, Jianhui Bai, Wilson H. Lee, Joseph D. Yesselman, Zhipeng Lu, Willem A. Velema

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-13 (2022)

Determination of RNA 3D structures in vivo is a challenging problem. Here, the authors describe a chemical crosslinking method (SHARC) that they use in combination with RNA sequencing to measure distances between nucleotides in RNA 3D structures.

Externí odkaz: https://doaj.org/article/23db3afbdb22411185c72fcffb493615

Optimized photochemistry enables efficient analysis of dynamic RNA structuromes and interactomes in genetic and infectious diseases

Autor: Kongpan Li, Jianhui Bai, Minjie Zhang, Zhipeng Lu, Jian-Fu Chen, Ryan van Damme, Maia L. Corpuz, Chengqing Yu, Wilson H. Lee, Willem A. Velema

Publikováno v: Nature Communications, 12, 1, pp. 1-14
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Nature Communications
Nature Communications, 12, 1-14

Direct determination of RNA structures and interactions in living cells is critical for understanding their functions in normal physiology and disease states. Here, we present PARIS2, a dramatically improved method for RNA duplex determination in viv

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af2963c475badbca632ffa4fdec0045c
https://doi.org/10.1038/s41467-021-22552-y