Zobrazeno 1 - 10
of 137
pro vyhledávání: '"Ryan T Gill"'
Autor:
Alaksh Choudhury, Jacob A Fenster, Reilly G Fankhauser, Joel L Kaar, Olivier Tenaillon, Ryan T Gill
Publikováno v:
Molecular Systems Biology, Vol 16, Iss 3, Pp n/a-n/a (2020)
Abstract Deep mutational scanning can provide significant insights into the function of essential genes in bacteria. Here, we developed a high‐throughput method for mutating essential genes of Escherichia coli in their native genetic context. We us
Externí odkaz:
https://doaj.org/article/5f2e06ad113d433aa65e1e649e871ef6
Publikováno v:
PLoS ONE, Vol 11, Iss 1, p e0146916 (2016)
Chemical genomics expands our understanding of microbial tolerance to inhibitory chemicals, but its scope is often limited by the throughput of genome-scale library construction and genotype-phenotype mapping. Here we report a method for rapid, paral
Externí odkaz:
https://doaj.org/article/b6e5748fb942459f966604ff5e987999
Publikováno v:
PLoS ONE, Vol 9, Iss 11, p e113820 (2014)
Advances in computational methods that allow for exploration of the combinatorial mutation space are needed to realize the potential of synthetic biology based strain engineering efforts. Here, we present Constrictor, a computational framework that u
Externí odkaz:
https://doaj.org/article/c3bbfab6359840c5ae913024b47964ba
Autor:
Tirzah Y Glebes, Nicholas R Sandoval, Philippa J Reeder, Katherine D Schilling, Min Zhang, Ryan T Gill
Publikováno v:
PLoS ONE, Vol 9, Iss 1, p e87540 (2014)
Advances in genomics have improved the ability to map complex genotype-to-phenotype relationships, like those required for engineering chemical tolerance. Here, we have applied the multiSCale Analysis of Library Enrichments (SCALEs; Lynch et al. (200
Externí odkaz:
https://doaj.org/article/4e4ccccb70ce4e4b98d3534d3d30ba63
Publikováno v:
PLoS ONE, Vol 8, Iss 1, p e55052 (2013)
A fundamental issue in the design and development of antimicrobials is the lack of understanding of complex modes of action and how this complexity affects potential pathways for resistance evolution. Bac8c (RIWVIWRR-NH(2)) is an 8 amino acid antimic
Externí odkaz:
https://doaj.org/article/5f73addc1f074a9da1bc8ed69d424315
Publikováno v:
PLoS ONE, Vol 8, Iss 8, p e71097 (2013)
Beyond their traditional role of wrapping DNA, histones display antibacterial activity to Gram-negative and -positive bacteria. To identify bacterial components that allow survival to a histone challenge, we selected resistant bacteria from homologou
Externí odkaz:
https://doaj.org/article/04b1dbfe51d648729b18f3344a812e92
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-16 (2023)
Abstract RNA polymerase (RNAP) is emblematic of complex biological systems that control multiple traits involving trade-offs such as growth versus maintenance. Laboratory evolution has revealed that mutations in RNAP subunits, including RpoB, are fre
Externí odkaz:
https://doaj.org/article/d16934fc926045d6b0a15469f358e7af
Autor:
Julie M Struble, Ryan T Gill
Publikováno v:
PLoS ONE, Vol 4, Iss 11, p e6576 (2009)
BACKGROUND:The ability of bacteria to rapidly evolve resistance to antibiotics is a critical public health problem. Resistance leads to increased disease severity and death rates, as well as imposes pressure towards the discovery and development of n
Externí odkaz:
https://doaj.org/article/f8177a1f2af14218b08ebbc168da7fe2
Autor:
Arsenios Vlassis, Tanja L. Jensen, Marina Mohr, Dominika J. Jedrzejczyk, Xiangyou Meng, Gergo Kovacs, Martí Morera-Gómez, Andrea Barghetti, Sergi Muyo Abad, Roland F. Baumgartner, Kedar N. Natarajan, Lars K. Nielsen, Tanya Warnecke, Ryan T. Gill
Publikováno v:
iScience, Vol 26, Iss 12, Pp 108287- (2023)
Summary: Discovery of genomic safe harbor sites (SHSs) is fundamental for multiple transgene integrations, such as reporter genes, chimeric antigen receptors (CARs), and safety switches, which are required for safe cell products for regenerative cell
Externí odkaz:
https://doaj.org/article/1ed7e3c6b32b4486a833882c76203d11
Autor:
Marina Mohr, Nkerorema Damas, Johanne Gudmand-Høyer, Katrine Zeeberg, Dominika Jedrzejczyk, Arsenios Vlassis, Martí Morera-Gómez, Sara Pereira-Schoning, Urška Puš, Anna Oliver-Almirall, Tanja Lyholm Jensen, Roland Baumgartner, Brian Tate Weinert, Ryan T. Gill, Tanya Warnecke
Publikováno v:
ACS Synthetic Biology. 12:375-389