Zobrazeno 1 - 10
of 221
pro vyhledávání: '"Ryan T Gill"'
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-16 (2023)
Abstract RNA polymerase (RNAP) is emblematic of complex biological systems that control multiple traits involving trade-offs such as growth versus maintenance. Laboratory evolution has revealed that mutations in RNAP subunits, including RpoB, are fre
Externí odkaz:
https://doaj.org/article/d16934fc926045d6b0a15469f358e7af
Autor:
Arsenios Vlassis, Tanja L. Jensen, Marina Mohr, Dominika J. Jedrzejczyk, Xiangyou Meng, Gergo Kovacs, Martí Morera-Gómez, Andrea Barghetti, Sergi Muyo Abad, Roland F. Baumgartner, Kedar N. Natarajan, Lars K. Nielsen, Tanya Warnecke, Ryan T. Gill
Publikováno v:
iScience, Vol 26, Iss 12, Pp 108287- (2023)
Summary: Discovery of genomic safe harbor sites (SHSs) is fundamental for multiple transgene integrations, such as reporter genes, chimeric antigen receptors (CARs), and safety switches, which are required for safe cell products for regenerative cell
Externí odkaz:
https://doaj.org/article/1ed7e3c6b32b4486a833882c76203d11
Autor:
Mamta Gupta, Matthew Wong, Kamran Jawed, Kamil Gedeon, Hannah Barrett, Marcelo Bassalo, Clifford Morrison, Danish Eqbal, Syed Shams Yazdani, Ryan T. Gill, Jiaqi Huang, Marc Douaisi, Jonathan Dordick, Georges Belfort, Mattheos A.G. Koffas
Publikováno v:
Metabolic Engineering Communications, Vol 15, Iss , Pp e00210- (2022)
The production of the biofuel, isobutanol, in E. coli faces limitations due to alcohol toxicity, product inhibition, product recovery, and long-term industrial feasibility. Here we demonstrate an approach of combining both in vivo with in vitro metab
Externí odkaz:
https://doaj.org/article/ac03648bb6c645638fbd2ddc4000accd
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Regulatory networks respond to environmental and genetic perturbations by reprogramming metabolism. Here the authors screen a library of 82 regulators with 110,120 mutations to map the regulatory network of 4000 genes.
Externí odkaz:
https://doaj.org/article/600753d8182544abaa6cc9ffdf23ce81
Autor:
Marina Mohr, Nkerorema Damas, Johanne Gudmand-Høyer, Katrine Zeeberg, Dominika Jedrzejczyk, Arsenios Vlassis, Martí Morera-Gómez, Sara Pereira-Schoning, Urška Puš, Anna Oliver-Almirall, Tanja Lyholm Jensen, Roland Baumgartner, Brian Tate Weinert, Ryan T. Gill, Tanya Warnecke
Publikováno v:
ACS Synthetic Biology. 12:375-389
Autor:
Cong Chen, Alaksh Choudhury, Shuanghong Zhang, Andrew D. Garst, Xin Song, Xunli Liu, Tao Chen, Ryan T. Gill, Zhiwen Wang
Publikováno v:
mSystems, Vol 5, Iss 2 (2020)
ABSTRACT It is important to expedite our understanding of antibiotic resistance to address the increasing numbers of fatalities and environmental pollution due to the emergence of antibiotic resistance and multidrug-resistant strains. Here, we combin
Externí odkaz:
https://doaj.org/article/8e0568820c9d484880741007f3b5bb0e
Autor:
Alaksh Choudhury, Jacob A Fenster, Reilly G Fankhauser, Joel L Kaar, Olivier Tenaillon, Ryan T Gill
Publikováno v:
Molecular Systems Biology, Vol 16, Iss 3, Pp n/a-n/a (2020)
Abstract Deep mutational scanning can provide significant insights into the function of essential genes in bacteria. Here, we developed a high‐throughput method for mutating essential genes of Escherichia coli in their native genetic context. We us
Externí odkaz:
https://doaj.org/article/5f2e06ad113d433aa65e1e649e871ef6
Publikováno v:
Metabolic Engineering Communications, Vol 3, Iss , Pp 227-233 (2016)
We previously introduced the LASER database (Learning Assisted Strain EngineeRing, https://bitbucket.org/jdwinkler/laser_release) (Winkler et al. 2015) to serve as a platform for understanding past and present metabolic engineering practices. Over th
Externí odkaz:
https://doaj.org/article/f6ea9c6cc6d9470a80744e8ebc3596f4
Autor:
Xin Song, Yangyang Zheng, Shuting Li, Alaksh Choudhury, Xunli Liu, Tao Chen, Ryan T. Gill, Zhiwen Wang
Publikováno v:
AIChE Journal. 69
Publikováno v:
Metabolic Engineering Communications, Vol 2, Iss , Pp 30-38 (2015)
The ability of metabolic engineers to conceptualize, implement, and evaluate strain designs has dramatically increased in the last decade. Unlike other engineering fields, no centralized, open-access, and easily searched repository exists for catalog
Externí odkaz:
https://doaj.org/article/9a31ee6f051c4d94a09b58349bb09076