Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Ryan T Fuchs"'
Autor:
Kristin A. Fluke, Ryan T. Fuchs, Yueh-Lin Tsai, Victoria Talbott, Liam Elkins, Hallie P. Febvre, Nan Dai, Eric J. Wolf, Brett W. Burkhart, Jackson Schiltz, G. Brett Robb, Ivan R. Corrêa, Thomas J. Santangelo
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-20 (2024)
Abstract RNAs are often modified to invoke new activities. While many modifications are limited in frequency, restricted to non-coding RNAs, or present only in select organisms, 5-methylcytidine (m5C) is abundant across diverse RNAs and fitness-relev
Externí odkaz:
https://doaj.org/article/af268ebdcd824d6989e97d95df96dc37
Publikováno v:
PLoS ONE, Vol 10, Iss 5, p e0126049 (2015)
High-throughput sequencing (HTS) has become a powerful tool for the detection of and sequence characterization of microRNAs (miRNA) and other small RNAs (sRNA). Unfortunately, the use of HTS data to determine the relative quantity of different miRNAs
Externí odkaz:
https://doaj.org/article/082215326d3e4e648be4b43590b54e05
Autor:
Ryan T. Fuchs, Jennifer L. Curcuru, Megumu Mabuchi, Audrey Noireterre, Peter R. Weigele, Zhiyi Sun, G. Brett Robb
Publikováno v:
Communications Biology, Vol 5, Iss 1, Pp 1-16 (2022)
Two distinct Cas12a orthologs (Cme and Yme) from metagenomic sources are characterized. The enzymes are found to be thermotolerant and are compared to three mesophilic Cas12a enzymes (Lba, Fno and Asp).
Externí odkaz:
https://doaj.org/article/2f051ab7df9a4bb69aca89664afee82f
Autor:
Giedrius Gasiunas, Joshua K. Young, Tautvydas Karvelis, Darius Kazlauskas, Tomas Urbaitis, Monika Jasnauskaite, Mantvyda M. Grusyte, Sushmitha Paulraj, Po-Hao Wang, Zhenglin Hou, Shane K. Dooley, Mark Cigan, Clara Alarcon, N. Doane Chilcoat, Greta Bigelyte, Jennifer L. Curcuru, Megumu Mabuchi, Zhiyi Sun, Ryan T. Fuchs, Ezra Schildkraut, Peter R. Weigele, William E. Jack, G. Brett Robb, Česlovas Venclovas, Virginijus Siksnys
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
A few bacterial Cas9 nucleases have been repurposed as genome editing tools. Here, the authors use bioinformatic and biochemical analyses to characterize 79 Cas9 proteins, revealing substantial functional diversity and thus expanding the available to
Externí odkaz:
https://doaj.org/article/eff8b92a7b8248269794dd870fd5600c
Publikováno v:
Journal of the American College of Emergency Physicians Open, Vol 3, Iss 2, Pp n/a-n/a (2022)
Externí odkaz:
https://doaj.org/article/1e981d54500e426dbf3177474cafe754
Publikováno v:
The American Journal of Emergency Medicine. 68:214.e3-214.e6
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3c4279089203865285c1046410777c25
https://doi.org/10.1016/j.ajem.2023.04.003
https://doi.org/10.1016/j.ajem.2023.04.003
Autor:
Justin Albers, Megumu Mabuchi, Ryan T. Fuchs, Prarthana Mohanraju, Richard van Kranenburg, Jennifer L. Curcuru, Ioannis Mougiakos, G. Brett Robb, John van der Oost
Publikováno v:
CRISPR Journal, 4(1), 82-91
CRISPR J
CRISPR Journal 4 (2021) 1
CRISPR J
CRISPR Journal 4 (2021) 1
The ability of CRISPR-Cas12a nucleases to function reliably in a wide range of species has been key to their rapid adoption as genome engineering tools. However, so far, Cas12a nucleases have been limited for use in organisms with growth temperatures
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef649b486c311acffb3f3104cae4722f
https://doi.org/10.1089/crispr.2020.0086
https://doi.org/10.1089/crispr.2020.0086
Autor:
Ryan T, Fuchs, Jennifer L, Curcuru, Megumu, Mabuchi, Audrey, Noireterre, Peter R, Weigele, Zhiyi, Sun, G Brett, Robb
Publikováno v:
Communications biology. 5(1)
CRISPR-Cas12a proteins are RNA-guided endonucleases that cleave invading DNA containing target sequences adjacent to protospacer adjacent motifs (PAM). Cas12a orthologs have been repurposed for genome editing in non-native organisms by reprogramming
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::d52de699594fead92ca25de292f5a59c
https://pubmed.ncbi.nlm.nih.gov/35388146
https://pubmed.ncbi.nlm.nih.gov/35388146
Autor:
Joshua K. Young, William E. Jack, Česlovas Venclovas, Clara M. Alarcon, Darius Kazlauskas, Zhenglin Hou, Virginijus Siksnys, Megumu Mabuchi, Tomas Urbaitis, Tautvydas Karvelis, G. Brett Robb, Mark Cigan, Shane K. Dooley, Monika Jasnauskaite, Giedrius Gasiunas, Ryan T. Fuchs, N. Doane Chilcoat, Greta Bigelyte, Mantvyda M. Grusyte, Ezra Schildkraut, Po-Hao Wang, Peter Weigele, Sushmitha Paulraj, Zhiyi Sun, Jennifer L. Curcuru
CRISPR-Cas9 nucleases are abundant in microbes. To explore this largely uncharacterized diversity, we applied cell-free biochemical screens to rapidly assess the protospacer adjacent motif (PAM) and guide RNA (gRNA) requirements of novel Cas9 protein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3f6cdbeb4449b33160674b8f8cb468bb
https://doi.org/10.1101/2020.04.29.066654
https://doi.org/10.1101/2020.04.29.066654
Publikováno v:
RNA. 25:35-44
Cas9 nuclease is the key effector of type II CRISPR adaptive immune systems found in bacteria. The nuclease can be programmed by a single guide RNA (sgRNA) to cleave DNA in a sequence-specific manner. This property has led to its widespread adoption
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::950f5f6766fc8cf2dcc8fb2817c475c1
https://doi.org/10.1261/rna.067355.118
https://doi.org/10.1261/rna.067355.118