Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Ryan Mehl"'
Publikováno v:
Bio-Protocol, Vol 14, Iss 16 (2024)
Generating protein conjugates using the bioorthogonal ligation between tetrazines and trans-cyclooctene groups avoids the need to manipulate cysteine amino acids; this ligation is rapid, site-specific, and stoichiometric and allows for labeling of pr
Externí odkaz:
https://doaj.org/article/876c2165354e4360880f771f06568c30
Publikováno v:
Bio-Protocol, Vol 13, Iss 21 (2023)
While site-specific translational encoding of phosphoserine (pSer) into proteins in Escherichia coli via genetic code expansion (GCE) technologies has transformed our ability to study phospho-protein structure and function, recombinant phospho-protei
Externí odkaz:
https://doaj.org/article/756991dced1342b9b312fb884db2f365
Publikováno v:
Bio-Protocol, Vol 13, Iss 17 (2023)
Externí odkaz:
https://doaj.org/article/ced81f1388d7422eae586fa558c61647
Publikováno v:
Bio-Protocol, Vol 12, Iss 21 (2022)
This protocol describes the recombinant expression of proteins in E. coli containing phosphoserine (pSer) installed at positions guided by TAG codons. The E. coli strains that can be used here are engineered with a ∆serB genomic knockout to produce
Externí odkaz:
https://doaj.org/article/9665de5b69224d0291bc1705e6139010
Autor:
Kari van Zee, Kayla Jara, P. Andrew Karplus, Richard Cooley, John Lueck, Bettye Maddux, Ryan Mehl
Publikováno v:
Journal of Biological Chemistry. 299:103265
Publikováno v:
Journal of Biological Chemistry. 299:103264
Publikováno v:
Journal of Biological Chemistry. 299:104070
Autor:
Daniel T. Infield, Grace Galles, Jason D. Galpin, Shivani Manikandan, Colin Clark, Richard Cooley, Ryan Mehl, Christopher A. Ahern
Publikováno v:
Biophysical Journal. 121:178a
Autor:
Ryan Mehl, Pascal A. Nelson, Kristen Thomas, Nathalie Perdomo, Maria Clara Franco, Marissa Fuse, Alvaro G. Estévez
Publikováno v:
Nitric Oxide. 42:135