Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Ryan McQuillen"'
Autor:
Zhixin Lyu, Atsushi Yahashiri, Xinxing Yang, Joshua W. McCausland, Gabriela M. Kaus, Ryan McQuillen, David S. Weiss, Jie Xiao
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-16 (2022)
FtsN promotes the inward synthesis of septal peptidoglycan (sPG) through the FtsWI complex during bacterial cell division. Here, Lyu et al. apply single-molecule microscopy on E. coli to show that FtsN proteins (I) move processively at a speed simila
Externí odkaz:
https://doaj.org/article/736e43fa29c241ae825e8f762cdebbd2
Autor:
Xin Chen, Jin Zhang, Christopher H. Bohrer, Jie Xiao, Matthew Wooten, Brian Tenner, Elijah Roberts, Brian Ross, Xiaoli Weng, Shreyasi Thakur, Xinxing Yang, Melike Lakadamyali, Ryan McQuillen
Publikováno v:
Nat Methods
Single-molecule localization microscopy (SMLM) relies on the blinking behavior of a fluorophore, which is the stochastic switching between fluorescent and dark states. Blinking creates multiple localizations belonging to the same fluorophore, confoun
Most bacteria lack membrane-enclosed organelles to compartmentalize cellular processes. In lieu of physical compartments, bacterial proteins are often recruited to macromolecular scaffolds at specific subcellular locations to carry out their function
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ef8d49d535748904bb7e361dd413e5d3
https://doi.org/10.1101/2022.05.01.490209
https://doi.org/10.1101/2022.05.01.490209
Autor:
Joshua W. McCausland, Ana De La Cruz, Catherine L. Grimes, Hai Liang, Kristen E. DeMeester, Cintia C. Santiago, Xinxing Yang, Zhixin Lyu, Polly J. Phillips-Mason, Jie Xiao, Piet A. J. de Boer, Ryan McQuillen
Publikováno v:
Nature microbiology
Synthesis of septal peptidoglycan (sPG) is crucial for bacterial cell division. FtsW, an indispensable component of the cell division machinery in all walled bacterial species, was recently identified in vitro as a peptidoglycan glycosyltransferase (
Autor:
Zhixin Lyu, Atsushi Yahashiri, Xinxing Yang, Joshua W. McCausland, Gabriela M. Kaus, Ryan McQuillen, David S. Weiss, Jie Xiao
Publikováno v:
Nature communications. 13(1)
FtsN plays an essential role in promoting the inward synthesis of septal peptidoglycan (sPG) by the FtsWI complex during bacterial cell division. How it achieves this role is unclear. Here we use single-molecule tracking to investigate FtsN’s dynam
Autor:
Ryan McQuillen, Atsushi Yahashiri, Joshua W. McCausland, Gabriela M. Kaus, David S. Weiss, Zhixin Lyu, Xinxing Yang, Jie Xiao
The FtsN protein of Escherichia coli and other proteobacteria is an essential and highly conserved bitopic membrane protein that triggers the inward synthesis of septal peptidoglycan (sPG) during cell division. Previous work has shown that the activa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::601ecca8e06cc53c2f056937b9c5ef6c
https://doi.org/10.1101/2021.08.23.457437
https://doi.org/10.1101/2021.08.23.457437
Autor:
Ryan McQuillen, Jie Xiao
Publikováno v:
Annu Rev Biophys
The FtsZ protein is a highly conserved bacterial tubulin homolog. In vivo, the functional form of FtsZ is the polymeric, ring-like structure (Z-ring) assembled at the future division site during cell division. While it is clear that the Z-ring plays
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6fd5ba3a5ad084b48ba9c04234c78bfb
https://europepmc.org/articles/PMC8610174/
https://europepmc.org/articles/PMC8610174/
Autor:
Xinxing Yang, Ryan McQuillen, Zhixin Lyu, Polly Phillips-Mason, Ana De La Cruz, Joshua W. McCausland, Hai Liang, Kristem E. DeMeester, Cintia C. Santiago, Catherine L. Grimes, Piet de Boer, Jie Xiao
During bacterial cell division, synthesis of new septal peptidoglycan (sPG) is crucial for successful cytokinesis and cell pole morphogenesis. FtsW, a SEDS (Shape, Elongation, Division and Sporulation) family protein and an indispensable component of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::feeb0935e6621be01d00980487c396ad
https://doi.org/10.1101/850073
https://doi.org/10.1101/850073
Publikováno v:
Molecular Microbiology. 105:721-740
Summary The cytoskeletal GTPase FtsZ assembles at midcell, recruits the division machinery, and directs envelope invagination for bacterial cytokinesis. ZapA, a conserved FtsZ-binding protein, promotes Z-ring stability and efficient division through
Autor:
Christopher H. Bohrer, Xinxing Yang, Xiaoli Weng, Brian Tenner, Shreyasi Thakur, Ryan McQuillen, Brian Ross, Matthew Wooten, Xin Chen, Melike Lakadamyali, Jin Zhang, Elijah Roberts, Jie Xiao
In single-molecule localization based super-resolution microscopy (SMLM), a fluorophore stochastically switches between fluorescent- and dark-states, leading to intermittent emission of fluorescence, a phenomenon known as blinking. Intermittent emiss
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d50611ac679354ef03cf7fc635f8db22
https://doi.org/10.1101/768051
https://doi.org/10.1101/768051