Zobrazeno 1 - 9 of 9 pro vyhledávání: '"Ryan A Pak"'
1
Akademický článek
Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors
Autor: Joseph M Replogle, Jessica L Bonnar, Angela N Pogson, Christina R Liem, Nolan K Maier, Yufang Ding, Baylee J Russell, Xingren Wang, Kun Leng, Alina Guna, Thomas M Norman, Ryan A Pak, Daniel M Ramos, Michael E Ward, Luke A Gilbert, Martin Kampmann, Jonathan S Weissman, Marco Jost
Publikováno v: eLife, Vol 11 (2022)
CRISPR interference (CRISPRi) enables programmable, reversible, and titratable repression of gene expression (knockdown) in mammalian cells. Initial CRISPRi-mediated genetic screens have showcased the potential to address basic questions in cell biol
Externí odkaz: https://doaj.org/article/af03cd98b4f34c128b98cd963fa2ba56
Zobrazit plný text záznamu
2
Akademický článek
Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation
Autor: Max A Horlbeck, Luke A Gilbert, Jacqueline E Villalta, Britt Adamson, Ryan A Pak, Yuwen Chen, Alexander P Fields, Chong Yon Park, Jacob E Corn, Martin Kampmann, Jonathan S Weissman
Publikováno v: eLife, Vol 5 (2016)
We recently found that nucleosomes directly block access of CRISPR/Cas9 to DNA (Horlbeck et al., 2016). Here, we build on this observation with a comprehensive algorithm that incorporates chromatin, position, and sequence features to accurately predi
Externí odkaz: https://doaj.org/article/c361ae56fb8042e39a85ee8aa1ee21a1
Zobrazit plný text záznamu
4
Adaptive exchange sustains cullin-RING ubiquitin ligase networks and proper licensing of DNA replication
Autor: Yaru Zhang, Marco Jost, Ryan A. Pak, Daniel Lu, Jing Li, Brett Lomenick, Spiros D. Garbis, Chi-Ming Li, Jonathan S. Weissman, James Russell Lipford, Raymond J. Deshaies
Publikováno v: Proceedings of the National Academy of Sciences of the United States of America. 119(36)
Cop9 signalosome (CSN) regulates the function of cullin–RING E3 ubiquitin ligases (CRLs) by deconjugating the ubiquitin-like protein NEDD8 from the cullin subunit. To understand the physiological impact of CSN function on the CRL network and cell p
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc36e57f6f93f9157316c013a44750ab
https://pubmed.ncbi.nlm.nih.gov/36037385
Zobrazit plný text záznamu
6
A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response
Autor: Min Y. Cho, Atray Dixit, Marco Jost, Andrew N. Gray, Max A. Horlbeck, James K. Nuñez, Jacqueline E. Villalta, Jonathan S. Weissman, Marco Y. Hein, Luke A. Gilbert, Yuwen Chen, Britt Adamson, Oren Parnas, Ryan A. Pak, Carol A. Gross, Thomas M. Norman, Aviv Regev
Publikováno v: PMC
Cell, vol 167, iss 7
Functional genomics efforts face tradeoffs between number of perturbations examined and complexity of phenotypes measured. We bridge this gap with Perturb-seq, which combines droplet-based single-cell RNA-seq with a strategy for barcoding CRISPR-medi
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c3e4c24839c7727cb125c7230b04e21
https://orcid.org/0000-0003-2193-9001
Zobrazit plný text záznamu
7
Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation
Autor: Jacqueline E. Villalta, Alexander P. Fields, Jacob E. Corn, Brittany Adamson, Yuwen Chen, Ryan A. Pak, Chong Yon Park, Max A. Horlbeck, Martin Kampmann, Jonathan S. Weissman, Luke A. Gilbert
Publikováno v: Kampmann, Martin; Gilbert, Luke; Horlbeck, MA; Gilbert, LA; Villalta, JE; Adamson, B; et al.(2016). Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/5b5017ck
eLife
eLife, Vol 5 (2016)
We recently found that nucleosomes directly block access of CRISPR/Cas9 to DNA (Horlbeck et al., 2016). Here, we build on this observation with a comprehensive algorithm that incorporates chromatin, position, and sequence features to accurately predi
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebc1acae2dcfbfd4d96e77865fdb9971
https://doi.org/10.7554/elife.19760
Zobrazit plný text záznamu
9
Activation of HIPK2 Promotes ER Stress-Mediated Neurodegeneration in Amyotrophic Lateral Sclerosis
Autor: Jonah R. Chan, Jinhua Wang, Kathy H. Li, Aaron D. Gitler, Amy A. Tang, John Q. Trojanowski, Alma L. Burlingame, Eileen H. Bigio, Anatoly Urisman, Eric J. Huang, Virginia M.-Y. Lee, William W. Seeley, Ana Jovičić, Teepu Siddique, Sebum Lee, Nathanael S. Gray, Stephanie A. Redmond, Yulei Shang, Ryan A. Pak
Publikováno v: Lee, S; Shang, Y; Redmond, SA; Urisman, A; Tang, AA; Li, KH; et al.(2016). Activation of HIPK2 Promotes ER Stress-Mediated Neurodegeneration in Amyotrophic Lateral Sclerosis. NEURON, 91(1), 41-55. doi: 10.1016/j.neuron.2016.05.021. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/98t7g7j0
Neuron, vol 91, iss 1
Persistent accumulation of misfolded proteins causes endoplasmic reticulum (ER) stress, a prominent feature in many neurodegenerative diseases including amyotrophic lateral sclerosis (ALS). Here we report the identification of homeodomain interacting
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9eab20a55403798d2005e9292d74cea3
https://pubmed.ncbi.nlm.nih.gov/27321923
Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání

Omezení vyhledávání
Plný text Recenzováno Digitální knihovna AV ČR
Zdroje