Zobrazeno 1 - 10
of 32
pro vyhledávání: '"Ruwan T. Kurulugama"'
Autor:
Varun V. Gadkari, Brock R. Juliano, Christopher S. Mallis, Jody C. May, Ruwan T. Kurulugama, John C. Fjeldsted, John A. McLean, David H. Russell, Brandon T. Ruotolo
Publikováno v:
The Analyst.
Native ion mobility-mass spectrometry (IM-MS) has emerged as an information-rich technique for gas phase protein structure characterization; however, IM resolution is currently insufficient for the detection of subtle structural differences in large
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1d8fb7db0947c02bb5a6ce4e59c3069
https://pubmed.ncbi.nlm.nih.gov/36537590
https://pubmed.ncbi.nlm.nih.gov/36537590
Publikováno v:
Anal Chem
Elucidating the structures and stabilities of proteins and their complexes is paramount to understanding their biological functions in cellular processes. Native mass spectrometry (MS) coupled with ion mobility spectrometry (IMS) is emerging as an im
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3308241d2e1be3d6d50d7c044546ea2
https://doi.org/10.1021/acs.analchem.0c00772
https://doi.org/10.1021/acs.analchem.0c00772
Autor:
Jill Coghlan, Jukyung Kang, John C. Fjeldsted, Daniel A. Polasky, Anna A. Schwendeman, Daniel D. Vallejo, Brandon T. Ruotolo, Ruwan T. Kurulugama, Carolina Rojas Ramírez
Publikováno v:
Analytical chemistry. 93(48)
Ion mobility-mass spectrometry (IM-MS) and collision-induced unfolding (CIU) assays of monoclonal antibody (mAb)-based biotherapeutics have proven sensitive to disulfide bridge structures, glycosylation patterns, and small molecule conjugation levels
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a2da71fc5899695404a5193c57ef0b0f
https://pubmed.ncbi.nlm.nih.gov/34808055
https://pubmed.ncbi.nlm.nih.gov/34808055
Autor:
Joseph D. Eschweiler, Daniel A. Polasky, Ruwan T. Kurulugama, Brandon T. Ruotolo, Daniel D. Vallejo, John C. Fjeldsted
Publikováno v:
Analytical Chemistry. 91:8137-8146
Collision-induced unfolding (CIU) of protein ions and their noncovalent complexes offers relatively rapid access to a rich portfolio of biophysical information, without the need to tag or purify proteins prior to analysis. Such assays have been chara
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d252c88554d7d98600761f4ad0e1d319
https://doi.org/10.1021/acs.analchem.9b00427
https://doi.org/10.1021/acs.analchem.9b00427
Autor:
Stephan Hann, Teresa Mairinger, Tim J. Causon, George Stafford, John C. Fjeldsted, Ruwan T. Kurulugama
Publikováno v:
Rapid Communications in Mass Spectrometry
RATIONALE The wide chemical diversity and complex matrices inherent to metabolomics still pose a challenge to current analytical approaches for metabolite screening. Although dedicated front-end separation techniques combined with high-resolution mas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c3c5239c6fdc505b37f340e7c33bf35
http://europepmc.org/articles/PMC6618165
http://europepmc.org/articles/PMC6618165
Autor:
Ruwan T. Kurulugama, Daniel D. Vallejo, Varun V. Gadkari, Brandon T. Ruotolo, Carolina Rojas Ramírez, John C. Fjeldsted
Publikováno v:
Anal Chem
Native ion mobility-mass spectrometry (IM-MS) is capable of revealing much that remains unknown within the structural proteome, promising such information on refractory protein targets. Here, we report the development of a unique drift tube IM-MS (DT
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d7ef48ad42c1779a6c06860cbd75505
https://pubmed.ncbi.nlm.nih.gov/33166123
https://pubmed.ncbi.nlm.nih.gov/33166123
Autor:
Julia Laskin, Veerupaksh Singla, Ruwan T. Kurulugama, Steve Creger, Daniela Mesa Sanchez, John C. Fjeldsted
Publikováno v:
Journal of the American Society for Mass Spectrometry. 31(12)
Mass spectrometry imaging (MSI) is a powerful technique for the label-free spatially resolved analysis of biological tissues. Coupling ion mobility (IM) separation with MSI allows for separation of isobars in the mobility dimension and increases conf
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4e906d659f698bc5b7693b68aa2f7f8
https://pubmed.ncbi.nlm.nih.gov/32841564
https://pubmed.ncbi.nlm.nih.gov/32841564
Autor:
Julia Laskin, Steve Creger, Ruwan T. Kurulugama, Veerupaksh Singla, Daniela Mesa Sanchez, John C. Fjeldsted
Mass spectrometry imaging (MSI) is a powerful technique for the label-free spatially-resolved analysis of biological tissues. Coupling ion mobility (IM) separation with MSI allows separation of isobars in the mobility dimension and increases confiden
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12d938357cde95246649c58fa3c1e5f3
https://doi.org/10.26434/chemrxiv.12408083
https://doi.org/10.26434/chemrxiv.12408083
Autor:
Ed Darland, Christian Klein, Alfred L. Yergey, Alex Mordehai, Stephanie M. Cologna, Ruwan T. Kurulugama, Peter S. Backlund, Paul S. Blank
Publikováno v:
The Analyst. 143:4147-4154
In this study, we have evaluated a low field limit drift tube ion mobility device for ion mobility-mass spectrometry (IM-MS) measurements that uses nitrogen as a bath gas with electrospray ionization on a modified Q-TOF instrument. We have determined
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::212be02a35ac74363e339cbe8bc9bb1a
https://doi.org/10.1039/c8an00646f
https://doi.org/10.1039/c8an00646f
Publikováno v:
Analytica Chimica Acta. 1163:338508
The focus of this work was the implementation of ion mobility (IM) and a prototype quadrupole driver within data independent acquisition (DIA) using a drift tube IM-QTOFMS aiming to improve the level of confidence in identity confirmation workflows f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4ba2ebf7ccc42082ae111b047fbcefa
https://doi.org/10.1016/j.aca.2021.338508
https://doi.org/10.1016/j.aca.2021.338508