Zobrazeno 1 - 10
of 96
pro vyhledávání: '"Ruud F Toonen"'
Autor:
Adlin Abramian, Rein I Hoogstraaten, Fiona H Murphy, Kathryn F McDaniel, Ruud F Toonen, Matthijs Verhage
Publikováno v:
eLife, Vol 13 (2024)
Neuropeptides and neurotrophins are stored in and released from dense core vesicles (DCVs). While DCVs and synaptic vesicles (SVs) share fundamental SNARE/SM proteins for exocytosis, a detailed understanding of DCV exocytosis remains elusive. We rece
Externí odkaz:
https://doaj.org/article/8df4ba1466d64e7c88f641f81fa7540e
Autor:
Aygul Subkhangulova, Miguel A Gonzalez-Lozano, Alexander JA Groffen, Jan RT van Weering, August B Smit, Ruud F Toonen, Matthijs Verhage
Publikováno v:
eLife, Vol 12 (2023)
Tomosyn is a large, non-canonical SNARE protein proposed to act as an inhibitor of SNARE complex formation in the exocytosis of secretory vesicles. In the brain, tomosyn inhibits the fusion of synaptic vesicles (SVs), whereas its role in the fusion o
Externí odkaz:
https://doaj.org/article/5b14d100d8134e01bd5d91d6962c2b54
Publikováno v:
eLife, Vol 11 (2022)
Different organelles traveling through neurons exhibit distinct properties in vitro, but this has not been investigated in the intact mammalian brain. We established simultaneous dual color two-photon microscopy to visualize the trafficking of Neurop
Externí odkaz:
https://doaj.org/article/bce6d8c0ca1d4f0997aca037cc6bccf7
Publikováno v:
PLoS Biology, Vol 18, Iss 8, p e3000826 (2020)
Ca2+/calmodulin-dependent kinase II (CaMKII) regulates synaptic plasticity in multiple ways, supposedly including the secretion of neuromodulators like brain-derived neurotrophic factor (BDNF). Here, we show that neuromodulator secretion is indeed re
Externí odkaz:
https://doaj.org/article/a14991662d144e3994c309510d0be9b7
Autor:
Aishwarya G Nadadhur, Javier Emperador Melero, Marieke Meijer, Desiree Schut, Gerbren Jacobs, Ka Wan Li, J J Johannes Hjorth, Rhiannon M Meredith, Ruud F Toonen, Ronald E Van Kesteren, August B Smit, Matthijs Verhage, Vivi M Heine
Publikováno v:
PLoS ONE, Vol 12, Iss 6, p e0178533 (2017)
Generation of neuronal cultures from induced pluripotent stem cells (hiPSCs) serve the studies of human brain disorders. However we lack neuronal networks with balanced excitatory-inhibitory activities, which are suitable for single cell analysis. We
Externí odkaz:
https://doaj.org/article/f7a60a49989a499583822389b705e4bf
Autor:
Margherita Farina, Rhea van de Bospoort, Enqi He, Claudia M Persoon, Jan RT van Weering, Jurjen H Broeke, Matthijs Verhage, Ruud F Toonen
Publikováno v:
eLife, Vol 4 (2015)
Externí odkaz:
https://doaj.org/article/61a34f8e436e4c4caf2ed5928dcc6d3e
Autor:
Margherita Farina, Rhea van de Bospoort, Enqi He, Claudia M Persoon, Jan RT van Weering, Jurjen H Broeke, Matthijs Verhage, Ruud F Toonen
Publikováno v:
eLife, Vol 4 (2015)
Neuropeptides released from dense-core vesicles (DCVs) modulate neuronal activity, but the molecules driving DCV secretion in mammalian neurons are largely unknown. We studied the role of calcium-activator protein for secretion (CAPS) proteins in neu
Externí odkaz:
https://doaj.org/article/6baa565981ab4bbf9fa333ddb9f1e0c3
Publikováno v:
PLoS ONE, Vol 10, Iss 9, p e0138950 (2015)
The munc18-1 gene encodes two splice-variants that vary at the C-terminus of the protein and are expressed at different levels in different regions of the adult mammalian brain. Here, we investigated the expression pattern of these splice variants wi
Externí odkaz:
https://doaj.org/article/5c5beee94bc6467ea4039d8365ead389
Publikováno v:
eLife, Vol 3 (2014)
Transmitter release at synapses is regulated by preceding neuronal activity, which can give rise to short-term enhancement of release like post-tetanic potentiation (PTP). Diacylglycerol (DAG) and Protein-kinase C (PKC) signaling in the nerve termina
Externí odkaz:
https://doaj.org/article/a873568ed3a04815ae44a1059b25932f
Publikováno v:
PLoS ONE, Vol 2, Iss 7, p e616 (2007)
Rab3a is a small GTPase that binds selectively to secretory vesicles and switches between active, GTP-bound and inactive, GDP-bound conformations. In yeast, Rab and SM-genes interact genetically to promote vesicle targeting/fusion. We tested differen
Externí odkaz:
https://doaj.org/article/1625adba209f4ee694fd8bbbd730c29f