Mapping localization of 21 endogenous proteins in the Golgi apparatus of rodent neurons

Autor: Danique M. van Bommel, Ruud F. Toonen, Matthijs Verhage

Publikováno v: Scientific Reports, Vol 13, Iss 1, Pp 1-9 (2023)

Abstract The Golgi apparatus is the major sorting hub in the secretory pathway and particularly important for protein sorting in neurons. Knowledge about protein localization in Golgi compartments is largely based on work in cell lines. Here, we syst

Externí odkaz: https://doaj.org/article/da4f431dcb64471086597fedb9d67fe1

Tomosyn affects dense core vesicle composition but not exocytosis in mammalian neurons

Autor: Aygul Subkhangulova, Miguel A Gonzalez-Lozano, Alexander JA Groffen, Jan RT van Weering, August B Smit, Ruud F Toonen, Matthijs Verhage

Publikováno v: eLife, Vol 12 (2023)

Tomosyn is a large, non-canonical SNARE protein proposed to act as an inhibitor of SNARE complex formation in the exocytosis of secretory vesicles. In the brain, tomosyn inhibits the fusion of synaptic vesicles (SVs), whereas its role in the fusion o

Externí odkaz: https://doaj.org/article/5b14d100d8134e01bd5d91d6962c2b54

Neuronal F-Box protein FBXO41 regulates synaptic transmission and hippocampal network maturation

Autor: Ana R.A.A. Quadros, Rocío Díez Arazola, Andrea Romaguera Álvarez, Johny Pires, Rhiannon M. Meredith, Ingrid Saarloos, Matthijs Verhage, Ruud F. Toonen

Publikováno v: iScience, Vol 25, Iss 4, Pp 104069- (2022)

Summary: FBXO41 is a neuron-specific E3 ligase subunit implicated in epileptic encephalopathies. Fbxo41 null mutant (KO) mice show behavioral deficits and early lethality. Here, we report that loss of FBXO41 causes defects in synaptic transmission an

Externí odkaz: https://doaj.org/article/260425520815456793f1b1359ddeb30b

CaMKII controls neuromodulation via neuropeptide gene expression and axonal targeting of neuropeptide vesicles.

Autor: Alessandro Moro, Geeske M van Woerden, Ruud F Toonen, Matthijs Verhage

Publikováno v: PLoS Biology, Vol 18, Iss 8, p e3000826 (2020)

Ca2+/calmodulin-dependent kinase II (CaMKII) regulates synaptic plasticity in multiple ways, supposedly including the secretion of neuromodulators like brain-derived neurotrophic factor (BDNF). Here, we show that neuromodulator secretion is indeed re

Externí odkaz: https://doaj.org/article/a14991662d144e3994c309510d0be9b7

Dysregulation of Synaptic and Developmental Transcriptomic/Proteomic Profiles upon Depletion of MUNC18-1

Autor: Annemiek A. Van Berkel, Frank Koopmans, Miguel Angel Gonzalez-Lozano, Hanna C. A. Lammertse, Femke Feringa, Julien Bryois, Patrick F. Sullivan, August B. Smit, Ruud F. Toonen, Matthijs Verhage

Publikováno v: eNeuro, 9(6):ENEURO.0186-22.2022. Society for Neuroscience
Van Berkel, A A, Koopmans, F, Gonzalez-Lozano, M A, Lammertse, H C A, Feringa, F, Bryois, J, Sullivan, P F, Smit, A B, Toonen, R F & Verhage, M 2022, ' Dysregulation of Synaptic and Developmental Transcriptomic/Proteomic Profiles upon Depletion of MUNC18-1 ', eNeuro, vol. 9, no. 6, pp. 1-14 . https://doi.org/10.1523/ENEURO.0186-22.2022
eNeuro, 9(6), 1-14. Society for Neuroscience
van Berkel, A A, Koopmans, F, Gonzalez-Lozano, M A, Lammertse, H C A, Feringa, F, Bryois, J, Sullivan, P F, Smit, A B, Toonen, R F & Verhage, M 2022, ' Dysregulation of Synaptic and Developmental Transcriptomic/Proteomic Profiles upon Depletion of MUNC18-1 ', eNeuro, vol. 9, no. 6, ENEURO.0186-22.2022 . https://doi.org/10.1523/ENEURO.0186-22.2022

Absence of presynaptic protein MUNC18-1 (gene:Stxbp1) leads to neuronal cell death at an immature stage before synapse formation. Here, we performed transcriptomic and proteomic profiling of immatureStxbp1knock-out (KO) cells to discover which cellul

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2b2c95da3fafb956d6ac1dd5fcc8a5d
https://doi.org/10.1523/ENEURO.0186-22.2022