Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Ruud Das"'
Autor:
Ruud Das, Rens Roosloot, Melissa van Pel, Koen Schepers, Marijn Driessen, Willem E. Fibbe, Joost Dick de Bruijn, Helene Roelofs
Publikováno v:
Journal of Translational Medicine, Vol 17, Iss 1, Pp 1-13 (2019)
Abstract Background Cell-based therapies have the potential to become treatment options for many diseases, but efficient scale-out of these therapies has proven to be a major hurdle. Bioreactors can be used to overcome this hurdle, but changing the c
Externí odkaz:
https://doaj.org/article/62daa1df1f634a9aa203429cec3a4b11
Publikováno v:
Annals of Anatomy, 198, 1. Urban und Fischer Verlag Jena
Annals of Anatomy-Anatomischer Anzeiger, 198, 1-10. Elsevier GmbH
Annals of Anatomy-Anatomischer Anzeiger, 198, 1-10. Elsevier GmbH
Culturing chondrocytes under oxygen tension closely resembling their in vivo environment has been shown to have positive effects on matrix synthesis. In redifferentiation of expanded chondrocytes, hypoxia increased collagen type II expression. Howeve
Publikováno v:
Tissue Engeneering Part B-Reviews, 16(2), 159-168. Mary Ann Liebert Inc.
Bone marrow-derived mesenchymal stem cells (MSCs) have demonstrated potential for regenerative medicine strategies. Knowledge of the way these cells respond to their environment in in vitro culture and after implantation in vivo is crucial for succes
Publikováno v:
Journal of Orthopaedic Research. 28:537-545
Effects of oxygen tension (pO(2)) and pH on gene and protein expression and metabolic activity of human chondrocytes were independently assessed. Chondrocytes were cultured under a range of pH (6.4-7.4) and different pO(2) (5 and 20%) during 5 days i
Publikováno v:
Journal of Orthopaedic Translation, Vol 7, Iss C, Pp 109-110 (2016)
Publikováno v:
Bio-Medical Materials and Engineering, 18(4-5), 279-282. IOS Press BV
Introduction (instead of abstract) Environmental parameters such as oxygen tension and extracellular pH are believed to play a crucial role in successful tissue engineering. Identifying the optimal values of such parameters and understanding the cell
Autor:
Ruud Das, J.C. van der Linden, Holger Jahr, G.J.V.M. van Osch, Harrie Weinans, Jan A N Verhaar
Publikováno v:
Osteoarthritis and Cartilage, 16(3), 385-391. W.B. Saunders
Objective: Expansion of autologous chondrocytes is a common step in procedures for cartilage defect repair. Subsequent dedifferentiation can alter cellular response to mechanical loading, having major consequences for the cell's behavior in vivo afte
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53b4f2fadb4296a2f33f6a75dbcac2ae
https://pure.eur.nl/en/publications/bc1020ed-bc19-4f31-a455-3ad6bb385421
https://pure.eur.nl/en/publications/bc1020ed-bc19-4f31-a455-3ad6bb385421
Publikováno v:
Osteoarthritis and Cartilage. 15
Autor:
Jan A N Verhaar, Nicole Kops, Tim J. M. Welting, Esther Haak, Holger Jahr, Niek P. van Til, Anna E. van der Windt, Harrie Weinans, Ruud Das, Marjolein M. J. Caron
Publikováno v:
Arthritis Research & Therapy
Arthritis Research & Therapy, 12(3). BioMed Central Ltd.
Arthritis Research & Therapy, 12(3). BioMed Central Ltd
Arthritis Research & Therapy, 12(3). BioMed Central Ltd.
Arthritis Research & Therapy, 12(3). BioMed Central Ltd
Introduction: Chondrocytes experience a hypertonic environment compared with plasma (280 mOsm) due to the high fixed negative charge density of cartilage. Standard isolation of chondrocytes removes their hypertonic matrix, exposing them to nonphysiol