Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Rutuja Kuhikar"'
Autor:
Jaganmay Sarkar, Shreya Dhepe, Amrita Shivalkar, Rutuja Kuhikar, Shruti More, Vijay Bhaskar Reddy Konala, Paresh Bhanushali, Amit Khanna
Publikováno v:
Stem Cell Research, Vol 77, Iss , Pp 103402- (2024)
We developed a well-characterized human induced pluripotent stem cell (iPSC) line obtained from healthy individuals' peripheral blood mononuclear cells (PBMC). The PBMCs were primed and reprogrammed using a non-integrating sendai viral vector, and th
Externí odkaz:
https://doaj.org/article/d1893f120355473c823bde0c0a349388
Publikováno v:
Stem Cell Research & Therapy, Vol 11, Iss 1, Pp 1-15 (2020)
Abstract Background Generation of red blood cells (RBCs) from hematopoietic stem cells (HSCs) in vitro takes about 21 days, making it unaffordable for clinical applications. Acceleration of the in vitro erythropoiesis process by using small molecules
Externí odkaz:
https://doaj.org/article/9c9dd64d1a27441ab8554f45ecab0832
Publikováno v:
Platelets, Vol 30, Iss 6, Pp 780-795 (2019)
Valproic acid (VPA) is one of the HDAC inhibitors used for the treatment of neurological disorders and hematological malignancies. Its role in self-renewal and proliferation of hematopoietic stem cells (HSCs) is well studied, but little is known abou
Externí odkaz:
https://doaj.org/article/690be29571454a388e54745f900125ff
Autor:
Rutuja Kuhikar, Nikhat Khan, Satyajeet P. Khare, Amit Fulzele, Sameer Melinkeri, Vaijayanti Kale, Lalita Limaye
Publikováno v:
Stem Cell Research, Vol 50, Iss , Pp 102150- (2021)
Neutrophils release neutrophil extracellular traps (NET) comprising of decondensed chromatin that immobilizes and kills pathogens. In vitro generation of neutrophils on a large scale from hematopoietic stem cells (HSCs) may be a useful strategy for t
Externí odkaz:
https://doaj.org/article/a9d866b56277400282479a6fcba4b135
Publikováno v:
Stem Cell Research & Therapy, Vol 11, Iss 1, Pp 1-15 (2020)
Stem Cell Research & Therapy
Stem Cell Research & Therapy
Background Generation of red blood cells (RBCs) from hematopoietic stem cells (HSCs) in vitro takes about 21 days, making it unaffordable for clinical applications. Acceleration of the in vitro erythropoiesis process by using small molecules could ev
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c113b6c5ad29844cfb02c1e6e4db4eb5
http://link.springer.com/article/10.1186/s13287-020-01603-z
http://link.springer.com/article/10.1186/s13287-020-01603-z
Autor:
Rutuja Kuhikar, Nikhat Khan, Philip, Joseph, Melinkeri, Sameer, Vaijayanti Kale, Limaye, Lalita
Figure S4. TGF-β1 causes cell cycle arrest during erythropoiesis process. a) Representative flow cytometry overlay showing cell cycle profile of control and TGF-β1 set on day 10 and day 12. TGF-β1 supplementation significantly increases the G0/G1
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c2fc00c219d58ec78485c16b335ac8e
Autor:
Rutuja Kuhikar, Nikhat Khan, Philip, Joseph, Melinkeri, Sameer, Vaijayanti Kale, Limaye, Lalita
Figure S3. Flow cytometry analysis of enucleation by SYTO16 staining. a) A representative flow panel showing SYTO16 profile of cultured cells and corresponding images of cells stained with Wright’s and Giemsa stain.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::393403b43f27435e45f49664355072cf
Autor:
Rutuja Kuhikar, Nikhat Khan, Philip, Joseph, Melinkeri, Sameer, Vaijayanti Kale, Limaye, Lalita
Figure S6. TGF-β1 enhances RBC production by inducing mitophagy. Representative flow cytometry overlay showing a) Cells viability by Calcein Am staining b) Mitochondrial mass c) Mitochondrial membrane potential and d) Mitochondrial ROS after SB-4315
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::026c3a863b218cd77151985548cf0432
Autor:
Rutuja Kuhikar, Nikhat Khan, Philip, Joseph, Melinkeri, Sameer, Vaijayanti Kale, Limaye, Lalita
Figure S2. TGF-β1 accelerates erythroid maturation. a) The graph shows the percentages of distinct erythroid stages at different time points determined by counting 500 cells from random fields of smears stained with Wright’s and Giemsa stain. b) R
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b6a77efa3185d17e032c010aad32bd39
Autor:
Rutuja Kuhikar, Nikhat Khan, Philip, Joseph, Melinkeri, Sameer, Vaijayanti Kale, Limaye, Lalita
Figure S1. Protocol for in vitro RBCs generation and cell viability assessed by MTT assay. a) Flow chart showing protocol used for in vitro generation of RBCs from HSCs obtained from APBL. b) Cell viability of TF1 cell line was evaluated by MTT assay
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06a1d9c7c274a9f2dcf769c9fa366051