Zobrazeno 1 - 10
of 25
pro vyhledávání: '"Ruth E Hanna"'
DYRK1A promotes viral entry of highly pathogenic human coronaviruses in a kinase-independent manner.
Autor:
Madison S Strine, Wesley L Cai, Jin Wei, Mia Madel Alfajaro, Renata B Filler, Scott B Biering, Sylvia Sarnik, Ryan D Chow, Ajinkya Patil, Kasey S Cervantes, Clayton K Collings, Peter C DeWeirdt, Ruth E Hanna, Kevin Schofield, Christopher Hulme, Silvana Konermann, John G Doench, Patrick D Hsu, Cigall Kadoch, Qin Yan, Craig B Wilen
Publikováno v:
PLoS Biology, Vol 21, Iss 6, p e3002097 (2023)
Identifying host genes essential for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has the potential to reveal novel drug targets and further our understanding of Coronavirus Disease 2019 (COVID-19). We previously performed a genome-wi
Externí odkaz:
https://doaj.org/article/c10dd58b10dd439eb9610eccf28fbb91
Publikováno v:
PLoS ONE, Vol 13, Iss 5, p e0197547 (2018)
Many implementations of pooled screens in mammalian cells rely on linking an element of interest to a barcode, with the latter subsequently quantitated by next generation sequencing. However, substantial uncoupling between these paired elements durin
Externí odkaz:
https://doaj.org/article/94664ffced444c179c128bb1023ef964
Autor:
Annabel K. Sangree, Audrey L. Griffith, Zsofia M. Szegletes, Priyanka Roy, Peter C. DeWeirdt, Mudra Hegde, Abby V. McGee, Ruth E. Hanna, John G. Doench
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-17 (2022)
Numerous rationally-designed and directed-evolution variants of SpCas9 have been reported to expand the utility of CRISPR technology. Here the authors make comparisons of numerous Cas9 variants, nominate options for base editing screens with denser c
Externí odkaz:
https://doaj.org/article/1a7462c71ec4497c818edebbbedb24ca
Autor:
Peter C. DeWeirdt, Annabel K. Sangree, Ruth E. Hanna, Kendall R. Sanson, Mudra Hegde, Christine Strand, Nicole S. Persky, John G. Doench
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
Isogenic pairs of cell lines are powerful tools but time-consuming to generate. Here the authors conduct genome-wide genetic interactions screens of ‘anchor’ genes with SaCas9 and SpCas9.
Externí odkaz:
https://doaj.org/article/892a16ea5f874784a2584fee9329b79a
Autor:
Krzysztof Chylinski, Maria Hubmann, Ruth E. Hanna, Connor Yanchus, Georg Michlits, Esther C. H. Uijttewaal, John Doench, Daniel Schramek, Ulrich Elling
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
Inducible genome editing systems often suffer from leakiness or reduced activity. Here the authors develop CRISPR-Switch, a Cre recombinase ON/OFF-controlled sgRNA cassette that allows consecutive editing of two loci.
Externí odkaz:
https://doaj.org/article/e256e98fa6a7453b8ef036e4dd9081b9
Autor:
Kendall R. Sanson, Ruth E. Hanna, Mudra Hegde, Katherine F. Donovan, Christine Strand, Meagan E. Sullender, Emma W. Vaimberg, Amy Goodale, David E. Root, Federica Piccioni, John G. Doench
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-15 (2018)
Genome-wide libraries for CRISPR knockout, interference, and activation have allowed the systemic interrogation of gene function. Here, the authors evaluate the Brunello CRISPRko library and introduce Dolcetto and Calabrese for CRISPRi and CRISPRa, r
Externí odkaz:
https://doaj.org/article/b2b3b6edfdcb4b859c371ceb1069d5c6
Autor:
Jin Wei, Ajinkya Patil, Clayton K. Collings, Mia Madel Alfajaro, Yu Liang, Wesley L. Cai, Madison S. Strine, Renata B. Filler, Peter C. DeWeirdt, Ruth E. Hanna, Bridget L. Menasche, Arya Ökten, Mario A. Peña-Hernández, Jon Klein, Andrew McNamara, Romel Rosales, Briana L. McGovern, M. Luis Rodriguez, Adolfo García-Sastre, Kris M. White, Yiren Qin, John G. Doench, Qin Yan, Akiko Iwasaki, Thomas P. Zwaka, Jun Qi, Cigall Kadoch, Craig B. Wilen
Publikováno v:
Nature Genetics. 55:471-483
Identification of host determinants of coronavirus infection informs mechanisms of viral pathogenesis and can provide new drug targets. Here we demonstrate that mammalian SWItch/Sucrose Non-Fermentable (mSWI/SNF) chromatin remodeling complexes, speci
Epithelial ovarian cancer (EOC) remains the most lethal gynecologic malignancy, largely due to metastasis and drug resistant recurrences. Fifteen percent of ovarian tumors carry mutations in BRCA1 or BRCA2, rendering them vulnerable to treatment with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c63a56d5ae7e711d80ba34df9ce6bf67
https://doi.org/10.20944/preprints202304.0327.v1
https://doi.org/10.20944/preprints202304.0327.v1
Autor:
Madison S. Strine, Wesley L. Cai, Jin Wei, Mia Madel Alfajaro, Renata B. Filler, Scott B. Biering, Sylvia Sarnik, Ajinkya Patil, Kasey S. Cervantes, Clayton K. Collings, Peter C. DeWeirdt, Ruth E. Hanna, Kevin Schofield, Christopher Hulme, Silvana Konermann, John G. Doench, Patrick D. Hsu, Cigall Kadoch, Qin Yan, Craig B. Wilen
Identifying host genes essential for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has the potential to reveal novel drug targets and further our understanding of coronavirus disease 2019 (COVID-19). We previously performed a genome-wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e4daff4ea1bbb6b966be856a427f6fc8
https://doi.org/10.1101/2022.09.13.507833
https://doi.org/10.1101/2022.09.13.507833
Autor:
J. Keith Joung, Peter C DeWeirdt, Marissa N Feeley, Kendall R Sanson, Teng Teng, Xuewen Pan, Audrey L Griffith, John G. Doench, Benjamin P. Kleinstiver, Mudra Hegde, Ruth E Hanna, Samantha M Borys, Alan Huang, Christine Strand, Annabel K Sangree
Publikováno v:
Nature biotechnology
Cas12a RNA-guided endonucleases are promising tools for multiplexed genetic perturbations because they can process multiple guide RNAs expressed as a single transcript and subsequently cleave target DNA. However, their widespread adoption has lagged