Increased stable integration efficiency in CHO cells through enhanced nuclear localization of Bxb1 serine integrase

Autor: Olli Huhtinen, Stuart Prince, Urpo Lamminmäki, Rune Salbo, Antti Kulmala

Publikováno v: BMC Biotechnology, Vol 24, Iss 1, Pp 1-12 (2024)

Abstract Background Mammalian display is an appealing technology for therapeutic antibody development. Despite the advantages of mammalian display, such as full-length IgG display with mammalian glycosylation and its inherent ability to select antibo

Externí odkaz: https://doaj.org/article/f63f603231cc46329248587277987296

Optimized Workflow for Selecting Peptides for HDX-MS Data Analyses

Autor: Rune Salbo, Lars Sørensen

Publikováno v: Sørensen, L & Salbo, R 2018, ' Optimized Workflow for Selecting Peptides for HDX-MS Data Analyses ', Journal of The American Society for Mass Spectrometry, vol. 29, no. 11, pp. 2278-2281 . https://doi.org/10.1007/s13361-018-2056-1

Hydrogen deuterium exchange measured by mass spectrometry (HDX-MS) is a commonly used technique for studying the structural dynamics of proteins in solution. The first part of any bottom-up HDX-MS experiment is to identify the peptides generated from

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::283c2b7930187dbd416f230907e25f3c
https://pubmed.ncbi.nlm.nih.gov/30171440

Electrochemical Reduction of Disulfide-Containing Proteins for Hydrogen/Deuterium Exchange Monitored by Mass Spectrometry

Autor: Rune Salbo, Michael Ploug, Thomas J. D. Jørgensen, Simon Mysling

Publikováno v: Mysling, S, Salbo, R, Ploug, M & Jørgensen, T J D 2014, ' Electrochemical reduction of disulfide-containing proteins for hydrogen/deuterium exchange monitored by mass spectrometry ', Analytical Chemistry, vol. 86, no. 1, pp. 340-345 . https://doi.org/10.1021/ac403269a

Characterization of disulfide bond-containing proteins by hydrogen/deuterium exchange monitored by mass spectrometry (HDX-MS) requires reduction of the disulfide bonds under acidic and cold conditions, where the amide hydrogen exchange reaction is qu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::698e2f6c0d11ec071d783558b548ecf1
https://doi.org/10.1021/ac403269a

Traveling-wave ion mobility mass spectrometry of protein complexes: accurate calibrated collision cross-sections of human insulin oligomers

Autor: Kim F. Haselmann, Ingrid Pettersson, Iain Campuzano, Matthew F. Bush, Helle Naver, Rune Salbo, Carol V. Robinson, Thomas J. D. Jørgensen

Publikováno v: Rapid Communications in Mass Spectrometry. 26:1181-1193

RATIONALE The collision cross-section (Ω) of a protein or protein complex ion can be measured using traveling-wave (T-wave) ion mobility (IM) mass spectrometry (MS) via calibration with compounds of known Ω. The T-wave Ω-values depend strongly on

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::227899c39cc21d6d0591c5739caf1cc5
https://doi.org/10.1002/rcm.6211

Traveling-wave ion mobility mass spectrometry of protein complexes: accurate calibrated collision cross-sections of human insulin oligomers

Autor: Rune, Salbo, Matthew F, Bush, Helle, Naver, Iain, Campuzano, Carol V, Robinson, Ingrid, Pettersson, Thomas J D, Jørgensen, Kim F, Haselmann

Publikováno v: Rapid communications in mass spectrometry : RCM. 26(10)

The collision cross-section (Ω) of a protein or protein complex ion can be measured using traveling-wave (T-wave) ion mobility (IM) mass spectrometry (MS) via calibration with compounds of known Ω. The T-wave Ω-values depend strongly on instrument

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a5e8210c2bf37c71d5642b5c6934d5c4
https://pubmed.ncbi.nlm.nih.gov/22499193