Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Ruhu Qi"'
Autor:
Ruhu Qi, Gottfried Otting
Publikováno v:
PLoS ONE, Vol 14, Iss 1, p e0211065 (2019)
The advent of high-fidelity DNA polymerases that can be used to linearize and amplify whole plasmids by PCR opened the door to greatly simplified cloning and mutagenesis protocols. Commercially available kits work well, but often have been optimized
Externí odkaz:
https://doaj.org/article/2f13e6f3abd24ed99db92c495dff54bd
Publikováno v:
PLoS Neglected Tropical Diseases, Vol 3, Iss 12, p e561 (2009)
BACKGROUND: The two-component NS2B-NS3 proteases of West Nile and dengue viruses are essential for viral replication and established targets for drug development. In all crystal structures of the proteases to date, the NS2B cofactor is located far fr
Externí odkaz:
https://doaj.org/article/03bf84fb7df64c1e9e2d14694380937c
Autor:
Shirley Siuley Lam, Ruhu Qi, Patrick Wang, Gottfried Otting, Margaret Sunde, Rezwan Siddiquee, Ann Hau-yu Kwan, Samuel Sung-chan Choi
Publikováno v:
Protein expression and purification. 170
Hydrophobins are a family of cysteine-rich proteins unique to filamentous fungi. The proteins are produced in a soluble form but self-assemble into organised amphipathic layers at hydrophilic:hydrophobic interfaces. These layers contribute to transit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa09359c17444dd2498ebbdccc23efe2
https://pubmed.ncbi.nlm.nih.gov/32032769
https://pubmed.ncbi.nlm.nih.gov/32032769
Publikováno v:
Angewandte Chemie. 124:2286-2289
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3701f85a3db5c1db4d9e0c077cbf0bb8
https://doi.org/10.1002/ange.201108275
https://doi.org/10.1002/ange.201108275
Publikováno v:
Angewandte Chemie International Edition. 51:2243-2246
The advent of efficient systems using genetic encoding for the site-specific incorporation of unnatural amino acids (UAA) into proteins has opened countless new possibilities for studying the structure, dynamics, and interactions of proteins. In part
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71b89049263bfb190593b1fee476a3dd
https://doi.org/10.1002/anie.201108275
https://doi.org/10.1002/anie.201108275
Publikováno v:
Journal of Biomolecular NMR. 50:35-42
Selectively isotope labelled protein samples can be prepared in vivo or in vitro from selectively labelled amino acids but, in many cases, metabolic conversions between different amino acids result in isotope scrambling. The best results are obtained
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09a50a68fe99297a3766f8dc91bda535
https://doi.org/10.1007/s10858-011-9477-5
https://doi.org/10.1007/s10858-011-9477-5
Autor:
Ruhu Qi, Peter John
Publikováno v:
Trends in Plant Science. 13:121-127
Currently, there is little information to indicate whether plant cell division and development is the collective effect of individual cell programming (cell-based) or is determined by organ-wide growth (organismal). Modulation of cell division does n
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::507dcc7efe15db31bf93a7ba838de001
https://doi.org/10.1016/j.tplants.2008.01.004
https://doi.org/10.1016/j.tplants.2008.01.004
Autor:
Kiyoshi, Ozawa, Ruhu, Qi
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1118
Cell-free protein synthesis (CFPS) offers a fast and inexpensive approach to selectively label proteins with isotopes that can then be detected by nuclear magnetic resonance (NMR) spectroscopy directly in the translation mixture. We describe a PCR-ba
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::94d237f2bdb59f0a9883389b173d4bee
https://pubmed.ncbi.nlm.nih.gov/24395416
https://pubmed.ncbi.nlm.nih.gov/24395416
Autor:
Ruhu Qi, Kiyoshi Ozawa
Publikováno v:
Methods in Molecular Biology ISBN: 9781627037815
Cell-free protein synthesis (CFPS) offers a fast and inexpensive approach to selectively label proteins with isotopes that can then be detected by nuclear magnetic resonance (NMR) spectroscopy directly in the translation mixture. We describe a PCR-ba
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bdf5fe2f9779e762f2275b7107636869
https://doi.org/10.1007/978-1-62703-782-2_11
https://doi.org/10.1007/978-1-62703-782-2_11
Autor:
Ruhu Qi, Peter John
Publikováno v:
Plant physiology. 144(3)
The Arabidopsis (Arabidopsis thaliana) CYCD2;1 gene introduced in genomic form increased cell formation in the Arabidopsis root apex and leaf, while generating full-length mRNA, raised CDK/CYCLIN enzyme activity, reduced G1-phase duration, and reduce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13f01e133e67288ac2c63f6e148c1d69
https://pubmed.ncbi.nlm.nih.gov/17513485
https://pubmed.ncbi.nlm.nih.gov/17513485