Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Ruediger Woscholski"'
Autor:
Peter J. Parker, Lodewijk V. Dekker, R Gigg, Ruediger Woscholski, J.A. Le Good, Ruth H. Palmer
Publikováno v:
Journal of Biological Chemistry. 270:22412-22416
As potential targets for polyphosphoinositides, activation of protein kinase C (PKC) isotypes (beta 1, epsilon, zeta, nu) and a member of the PKC-related kinase (PRK) family, PRK1, has been compared in vitro. PRK1 is shown to be activated by both pho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::89916207a9c672487996e24c6ef5874f
https://doi.org/10.1074/jbc.270.38.22412
https://doi.org/10.1074/jbc.270.38.22412
Publikováno v:
Journal of Biological Chemistry. 269:25067-25072
The regulatory (p85 alpha) and catalytic (p110 alpha) subunits of the mammalian phosphatidylinositol 3-kinase have been expressed in insect cells using the baculovirus sytem. The free catalytic subunit p110 alpha and the coexpressed heterodimeric com
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5577662c10c66b0553e41a285a1ce659
https://doi.org/10.1016/s0021-9258(17)31499-0
https://doi.org/10.1016/s0021-9258(17)31499-0
Autor:
Ruediger Woscholski, Dieter Marmé
Publikováno v:
Biochemical and Biophysical Research Communications. 186:1588-1593
Summary An endogenous protein inhibits the PKA-phosphorylation of the DHP-binding calcium channel complex in vitro. The inhibitory activity could be reduced by a treatment with detergents or dithiothreitol. Further purification separates the inhibito
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72977f9157663a5a2503e17b3fd7e81f
https://doi.org/10.1016/s0006-291x(05)81589-x
https://doi.org/10.1016/s0006-291x(05)81589-x
Publikováno v:
Biochemical and Biophysical Research Communications. 182:947-952
Binding studies using the calcium channel blockers omega-conotoxin and dihydropyridine revealed a rather equal amount of binding sites in brain from adult chicken. The omega-conotoxin binding sites could be solubilized using digitonin, without substa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5a94492e69714ad18d43d8f9b41c72e
https://doi.org/10.1016/0006-291x(92)91823-9
https://doi.org/10.1016/0006-291x(92)91823-9
Publikováno v:
Biochemistry. 34(36)
The substrate specificity of the purified, mammalian phosphatidylinositol 3-kinase is subject to modulation by detergents, which are able to switch substrate specificity in vitro in favor of PtdInsP2. This effect of the detergents is due to an activa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23db09b373849bfbf714a77885e5d1dd
https://pubmed.ncbi.nlm.nih.gov/7547877
https://pubmed.ncbi.nlm.nih.gov/7547877
Autor:
Nicholas J. Brooks, Piers R. J. Gaffney, Edward W. Tate, Ruediger Woscholski, Richard H. Templer, Oscar Ces, Annela M. Seddon, Samuel Furse
Publikováno v:
Soft Matter. 8:3090
In this paper we present evidence that phosphatidylinositol 4-phosphate induces curvature in biological membranes. The phase behaviour of mixtures of distearoylphosphatidylinositol 4-phosphate (DSPIP) and dioleoylphosphatidylcholine (DOPC) as a funct
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bbd8ca8bdcbddd74e9b24b8b5c9a74cd
https://doi.org/10.1039/c2sm07358g
https://doi.org/10.1039/c2sm07358g
Autor:
Dieter Marmé, Ruediger Woscholski
Publikováno v:
Biochemical and biophysical research communications. 182(1)
Rabbit skeletal muscle membranes contain a protein which inhibits the cAMP-dependent protein kinase. The activity of the partially purified membrane protein is characterized by an IC-50 of 10 to 30 nM with respect to the inhibition of the activity of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::389438d349b4232e17bcdf2b68f60660
https://pubmed.ncbi.nlm.nih.gov/1731783
https://pubmed.ncbi.nlm.nih.gov/1731783
Publikováno v:
FEBS Letters. (2):109-114
The mammalian Ptdlns 3-kinase is shown to be inhibited by low nanomolar concentrations of demethoxyviridin, an antifungal agent structurally related to wortmannin. The inhibitory potency of both compounds could be observed in purified Ptdlns 3-kinase
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd798ede9f9061af01678ff6ce5ed692