Zobrazeno 1 - 10
of 40
pro vyhledávání: '"Ruedi Meili"'
Autor:
Juan C del Álamo, Ruedi Meili, Begoña Álvarez-González, Baldomero Alonso-Latorre, Effie Bastounis, Richard Firtel, Juan C Lasheras
Publikováno v:
PLoS ONE, Vol 8, Iss 9, p e69850 (2013)
We introduce a novel three-dimensional (3D) traction force microscopy (TFM) method motivated by the recent discovery that cells adhering on plane surfaces exert both in-plane and out-of-plane traction stresses. We measure the 3D deformation of the su
Externí odkaz:
https://doaj.org/article/b88e9891dcea49e397f665003b71c15b
Autor:
Adithan Kandasamy, Shu Chien, Ruedi Meili, Yi-Ting Yeh, Juan C. del Álamo, Joshua Francois, Cindy Ayala, Amy B Schwartz, Juan C. Lasheras
Publikováno v:
Science Advances
Neutrophils use their ability to deform surrounding extracellular matrix and turn to navigate through complex 3D environments.
Neutrophils migrating through extravascular spaces must negotiate narrow matrix pores without losing directional movem
Neutrophils migrating through extravascular spaces must negotiate narrow matrix pores without losing directional movem
Autor:
Juan C. del Álamo, Begoña Álvarez-González, Effie Bastounis, Juan C. Lasheras, Ruedi Meili, Richard A. Firtel
Publikováno v:
Biophysical Journal. 108:821-832
Fast amoeboid migration requires cells to apply mechanical forces on their surroundings via transient adhesions. However, the role these forces play in controlling cell migration speed remains largely unknown. We used three-dimensional force microsco
Autor:
Juan C. del Álamo, Ruedi Meili, Begoña Álvarez-González, Shun Zhang, Manuel Gomez-Gonzalez, Richard A. Firtel, Juan C. Lasheras
Publikováno v:
Scientific reports, vol 7, iss 1
Scientific Reports
Scientific Reports
Cellular traction force microscopy (TFM) requires knowledge of the mechanical properties of the substratum where the cells adhere to calculate cell-generated forces from measurements of substratum deformation. Polymer-based hydrogels are broadly used
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ddaa14d3ad9112d4106f58129fcd93a
https://escholarship.org/uc/item/28f5x7br
https://escholarship.org/uc/item/28f5x7br
Autor:
Juan C. Lasheras, Baldomero Alonso-Latorre, Ruedi Meili, Richard A. Firtel, Juan C. del Álamo
Publikováno v:
Cellular and Molecular Bioengineering
We used principal component analysis to dissect the mechanics of chemotaxis of amoeboid cells into a reduced set of dominant components of cellular traction forces and shape changes. The dominant traction force component in wild-type cells accounted
Autor:
Effie Bastounis, Baldomero Alonso-Latorre, Juan C. del Álamo, Ruedi Meili, Juan C. Lasheras, Richard A. Firtel
Publikováno v:
Molecular Biology of the Cell
A combination of traction force and F-actin measurements shows that cells lacking either of the SCAR/WAVE complex proteins SCAR and PIR121 exhibit an altered cell motility cycle and spatiotemporal distribution of tractions stresses, which correlate i
Autor:
Ruedi Meili, Juan C. Lasheras, Baldomero Alonso-Latorre, Richard A. Firtel, Juan C. del Álamo
Publikováno v:
Molecular Biology of the Cell
Amoeboid motility results from pseudopod protrusions and retractions driven by traction forces of cells. We propose that the motor and actin-crosslinking functions of MyoII differentially control the temporal and spatial distribution of the traction
Autor:
Richard A. Firtel, Kosuke Takeda, Pascale G. Charest, Peter N. Devreotes, Atsuo T. Sasaki, Lauren W. Sundheimer, Susan Lee, Ruedi Meili, Chris Janetopoulos
Publikováno v:
The Journal of Cell Biology
Phosphoinositide 3-kinase (PI3K)gamma and Dictyostelium PI3K are activated via G protein-coupled receptors through binding to the Gbetagamma subunit and Ras. However, the mechanistic role(s) of Gbetagamma and Ras in PI3K activation remains elusive. F
Publikováno v:
The EMBO Journal. 23:4177-4189
Chemotaxis requires localized F-actin polymerization at the site of the plasma membrane closest to the chemoattractant source, a process controlled by Rac/Cdc42 GTPases. We identify Dictyostelium RacB as an essential mediator of this process. RacB is
Autor:
Sylvain Merlot, David J. Pagliarini, Richard A. Firtel, Tomohiko Maehama, Jack E. Dixon, Ruedi Meili
Publikováno v:
Journal of Biological Chemistry. 278:39866-39873
Phosphoinositides play important roles as signaling molecules in different cell compartments by regulating the localization and activity of proteins through their interaction with specific domains. The activity of these lipids depends on which sites