Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Rudolf Seibl"'
Autor:
Josef Eberle, Rudolf Seibl
Publikováno v:
Journal of Virological Methods. 40:347-356
A new and sensitive assay of reverse transcriptase (RT) activity of retroviruses measures the incorporation of digoxigenin-labelled dUTP in newly synthesized DNA instead of radioactively labelled (3H- or 32P-)dTTP. To avoid difficulties associated wi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b85301dc0216756bef10d121f9615e2
https://doi.org/10.1016/0166-0934(92)90092-r
https://doi.org/10.1016/0166-0934(92)90092-r
Publikováno v:
Analytical Biochemistry. 192:222-231
A procedure for the nonradioactive labeling of oligonucleotides with the hapten digoxigenin (DIG) has been developed. The label is introduced by enzymatic tailing of the 3'-end. Two different modified nucleotides were applied. DIG-dUTP allows the syn
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e6e7231844d4741363e5db2d94035b8a
https://doi.org/10.1016/0003-2697(91)90212-c
https://doi.org/10.1016/0003-2697(91)90212-c
Autor:
Rudolf SEIBL, Hans-Joachim HÖLTKE, Rüdiger RÜGER, Alfons MEINDL, Hans G. ZACHAU, Rudolf RAßHOFER, Michael ROGGENDORF, Hans WOLF, Norbert ARNOLD, Johannes WIENBERG, Christoph KESSLER
Publikováno v:
Biological Chemistry Hoppe-Seyler. 371:939-952
The digoxigenin-based non-radioactive DNA labeling and detection system was applied in various hybridization protocols using digoxigenin-labeled probes obtained by enzymatic incorporation of Dig-[11]-dUTP. In genomic blots single-copy genes (human ti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3e198d03c90f8cf2fb53add67badce2
https://doi.org/10.1515/bchm3.1990.371.2.939
https://doi.org/10.1515/bchm3.1990.371.2.939
Publikováno v:
Biological Chemistry Hoppe-Seyler. 371:929-938
The random-primed DNA labeling technique was modified for the incorporation of digoxigenin into DNA as basic component of the digoxigenin-based non-radioactive DNA labeling and detection system. Digoxigenin molecules act as reporter groups for highly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c96f26213fd2def77153aea245b67cc
https://doi.org/10.1515/bchm3.1990.371.2.929
https://doi.org/10.1515/bchm3.1990.371.2.929
Autor:
Gregor Sagner, Christoph Kessler, Josef Burg, Rüdiger Rüger, Wolfgang Hösel, G.G. Schmitz, Rudolf Seibl, Klaus Mühlegger, Thomas Walter, Anton Haselbeck, Hans-Joachim Höltke
Publikováno v:
Nonradioactive Labeling and Detection of Biomolecules ISBN: 9783662001462
The digoxigenin:anti-dioxigenin (DIG) indicator system is based on the specific interaction between the cardenolide steroid DIG, a chemically derived aglycon of digoxin and lanatoside C (see figure), and a high-affinity, DIG-specific antibody (Kessle
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::367c593f3ebb9cc9f3f6213cfe32766e
https://doi.org/10.1007/978-3-662-00144-8_3
https://doi.org/10.1007/978-3-662-00144-8_3
Autor:
Hans Söderlund, Kai Korpela, Francois Coutlée, Robert H. Yolken, Raphael P. Viscidi, Mary Collins, Richard A. Cardullo, Rudolf Seibl, Stefanie Koehler
Publikováno v:
Söderlund, H, Korpela, K, Coutlée, F, Yolken, R, Viscidi, R P, Collins, M, Cardullo, R A, Seibl, R & Koehler, S 1992, Quantitative Formats . in C Kessler (ed.), Nonradioactive Labeling and Detection of Biomolecules . Springer, Springer Laboratory, pp. 393-427 . https://doi.org/10.1007/978-3-662-00144-8_22
Nonradioactive Labeling and Detection of Biomolecules ISBN: 9783662001462
Nonradioactive Labeling and Detection of Biomolecules ISBN: 9783662001462
In most applications of hybridization assays the target nucleic acid is immobilized onto a filter, where it is then allowed to react with a labeled probe. In the sandwich hybridization reaction, the target sequence is kept in solution while allowed t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7427273c4fa5d9d1a07591064023136
https://cris.vtt.fi/en/publications/28d85f45-f124-4f33-ba3c-e59819d41f3c
https://cris.vtt.fi/en/publications/28d85f45-f124-4f33-ba3c-e59819d41f3c