Zobrazeno 1 - 10
of 27
pro vyhledávání: '"Rudolf Gilmanshin"'
Autor:
Eric J. White, Sergey V. Fridrikh, Ekaterina Protozanova, Dirk Peter Ten Broeck, Douglas B. Cameron, Rudolf Gilmanshin, Emilia Mollova, Meng Zhang
Publikováno v:
Analytical Biochemistry. 402:83-90
Here we describe bacterial genotyping by direct linear analysis (DLA) single-molecule mapping. DLA involves preparation of restriction digest of genomic DNA labeled with a sequence-specific fluorescent probe and stained nonspecifically with intercala
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41402c6687ce1bbf16dd4cfdc19933eb
https://doi.org/10.1016/j.ab.2010.03.024
https://doi.org/10.1016/j.ab.2010.03.024
Publikováno v:
Analytical Biochemistry. 391:135-143
Existing methods for extraction and processing of large fragments of bacterial genomic DNA are manual, time-consuming, and prone to variability in DNA quality and recovery. To solve these problems, we have designed and built an automated fluidic syst
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b3b0f2691b2b56129db7c7069f865c8
https://doi.org/10.1016/j.ab.2009.05.008
https://doi.org/10.1016/j.ab.2009.05.008
Autor:
Kimberly A. Gillis, Michael Gallo, Gregory R. Yantz, Steven R. Gullans, Nuno Goncalves, Christina M. D’Antoni, Rudolf Gilmanshin, Kevin M. Phillips, Lori Anne Neely, Jonathan W. Larson
Publikováno v:
Nucleic Acids Research
Herein we describe the first application of direct linear analysis (DLA) to the mapping of a bacterial artificial chromosome (BAC), specifically the 185.1 kb-long BAC 12M9. DLA is a single molecule mapping technology, based on microfluidic elongation
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa398ab7e877b26950b6c3e83bc301bd
https://doi.org/10.1093/nar/gki895
https://doi.org/10.1093/nar/gki895
Autor:
Nuno Goncalves, Eugene Y. Chan, Amie Jo Hatch, Steven R. Gullans, Eugene D. Carstea, Gordon G. Wong, Rudolf Gilmanshin, Jonathan W. Larson, Anthony M. Maletta, Rebecca A. Haeusler, Gregory R. Yantz, Martin Fuchs
Publikováno v:
Genome Research. 14:1137-1146
We have developed a rapid molecular mapping technology—Direct Linear Analysis (DLA)—on the basis of the analysis of individual DNA molecules bound with sequence-specific fluorescent tags. The apparatus includes a microfluidic device for stretchin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::151e110fbbd8cb0d08140bdb18bbffde
https://doi.org/10.1101/gr.1635204
https://doi.org/10.1101/gr.1635204
Publikováno v:
The Journal of the Acoustical Society of America. 141:3505-3505
Chromatographic purification of target biomolecules is an important downstream process step in the development of new therapeutic agents, such as antibodies. This step employs chromatographic processes, such as affinity separation utilizing Protein A
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::98610a99f76c329f55ebf2da4cc634a1
https://doi.org/10.1121/1.4987343
https://doi.org/10.1121/1.4987343
Publikováno v:
The Journal of Physical Chemistry B. 103:3947-3953
We have studied the thermal denaturation of holo α-lactalbumin (α-LA) using FTIR spectroscopy by monitoring the absorbance of protein bands in the secondary structure sensitive amide I region. In agreement with results obtained by other techniques,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6bc133d32bcdb4f39d5a79b5cfc43762
https://doi.org/10.1021/jp984518p
https://doi.org/10.1021/jp984518p
Publikováno v:
Accounts of Chemical Research. 31:709-716
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::030bbac2752f0d17f15e67e7b512cef8
https://doi.org/10.1021/ar970343a
https://doi.org/10.1021/ar970343a
Publikováno v:
Journal of Raman Spectroscopy. 29:15-21
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1021efb39417593645447631b61d45f5
https://doi.org/10.1002/(sici)1097-4555(199801)29:1<15::aid-jrs211>3.0.co
https://doi.org/10.1002/(sici)1097-4555(199801)29:1<15::aid-jrs211>3.0.co
Publikováno v:
The Journal of Physical Chemistry. 100:16754-16760
The isolated S-peptide (enzymatically cleaved 1−20 fragment of the ribonuclease, RNase S) is partly helical in solution. Both S-peptide and its truncated 1−15 analogue pep01 can combine with S-protein (residual part of RNase S without S-peptide),
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::28164a4185d5524d181bdff5c00c6972
https://doi.org/10.1021/jp9611941
https://doi.org/10.1021/jp9611941
Autor:
Jeffrey R. Krogmeier, Bryan Crane, Griffis Joshua, Lisa W. Kwok, Rudolf Gilmanshin, Nanor Kojanian, Michelle K. Nahas, Qun Zhong, Vyacheslav Papkov, Saad Shaikh, Richard Allen, Gene Malkin, Kedar Vyavahare, Robert H. Meltzer, Jonathan W. Larson, Yi Zhou, Linda Knaian
Publikováno v:
Lab on a chip. 11(5)
Rapid, specific, and sensitive detection of airborne bacteria, viruses, and toxins is critical for biodefense, yet the diverse nature of the threats poses a challenge for integrated surveillance, as each class of pathogens typically requires differen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7225b18afc68c0c7d8513bad723047fc
https://pubmed.ncbi.nlm.nih.gov/21249264
https://pubmed.ncbi.nlm.nih.gov/21249264