Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Rudo Grimm"'
Publikováno v:
Biophysical Journal. 76(6):3158-3166
Cryoelectron microscopy has been used to study the reorganization of unilamellar cationic lipid vesicles upon the addition of DNA. Unilamellar DNA-coated vesicles, as well as multilamellar DNA lipid complexes, could be observed. Also, DNA induced fus
Publikováno v:
Journal of Microscopy. 190:339-349
Fourier ring correlation and root-mean-square contrast of pairs of images, taken under identical conditions, were used as criteria of image quality for comparing unfiltered with zero-loss energy-filtered imaging using a TEM equipped with a post-colum
Publikováno v:
Journal of Microscopy. 183:60-68
Electron cryomicroscopy combined with energy filtering can be performed under low-dose conditions using a post-column energy filter. The microscope combined with the filter is set up such that it can be used with similar ease as a conventional micros
Publikováno v:
Trends in cell biology. 9(2)
Electron tomography is the most widely applicable method for obtaining three-dimensional information by electron microscopy. It is, in fact, the only method suitable for investigating pleomorphic structures, such as many supramolecular assemblies, or
Autor:
Wolfgang Baumeister, Noriko Tamura, Tomohiro Tamura, Abraham J. Koster, Jochen Walz, Rudo Grimm
Publikováno v:
Molecular cell. 1(1)
Tricorn protease is the core enzyme of a recently discovered modular proteolytic system. We present evidence that tricorn protease exists in vivo in the form of a higher-order assembly, namely as an icosahedral capsid. Its size exceeds that of many v
Publikováno v:
Biophysical journal. 72(1)
Semiautomatic single-axis tilt electron tomography has been used to visualize the three-dimensional organization of actin filaments in "phantom cells," i.e. lipid vesicles. The instrumentation consisted of a 120-kV electron microscope equipped with a
Publikováno v:
Ultramicroscopy. 63(3-4)
Using electron microscopy, the thickness of ice-embedded vesicles is estimated examining tilted and untilted views and assuming an ellipsoidal shape of the vesicles that appear to be circular in the untilted view. Another thickness measure is obtaine
Autor:
Dieter Typke, Wolfgang Baumeister, Reinhard Rachel, Hapreet Singh, Wolfram Zillig, Rudo Grimm
Publikováno v:
Biophysical Journal. (2):1031-1042
Whole cells of archaea were embedded in vitreous ice by plunge freezing and investigated by automated energy-filtered electron tomography at 120 kV. The embedded cells were between 300 and 750 nm thick, and their structures were reconstructed to a re