Fab-Arm Exchange Combined with Selective Protein A Purification Results in a Platform for Rapid Preparation of Monovalent Bispecific Antibodies Directly from Culture Media.

Autor: Steinhardt J; Antibody Discovery and Protein Engineering, AstraZeneca, One MedImmune Way, Gaithersburg, MD 201878, USA., Wu Y; Antibody Discovery and Protein Engineering, AstraZeneca, One MedImmune Way, Gaithersburg, MD 201878, USA., Fleming R; Antibody Discovery and Protein Engineering, AstraZeneca, One MedImmune Way, Gaithersburg, MD 201878, USA., Ruddle BT; Antibody Discovery and Protein Engineering, AstraZeneca, One MedImmune Way, Gaithersburg, MD 201878, USA., Patel P; Antibody Discovery and Protein Engineering, AstraZeneca, One MedImmune Way, Gaithersburg, MD 201878, USA., Wu H; Antibody Discovery and Protein Engineering, AstraZeneca, One MedImmune Way, Gaithersburg, MD 201878, USA., Gao C; Antibody Discovery and Protein Engineering, AstraZeneca, One MedImmune Way, Gaithersburg, MD 201878, USA., Dimasi N; Antibody Discovery and Protein Engineering, AstraZeneca, One MedImmune Way, Gaithersburg, MD 201878, USA.

Publikováno v: Pharmaceutics [Pharmaceutics] 2019 Dec 18; Vol. 12 (1). Date of Electronic Publication: 2019 Dec 18.

Design and characterization of homogenous antibody-drug conjugates with a drug-to-antibody ratio of one prepared using an engineered antibody and a dual-maleimide pyrrolobenzodiazepine dimer.

Autor: White JB; a Antibody Discovery and Protein Engineering , MedImmune , Gaithersburg , MD , USA., Fleming R; a Antibody Discovery and Protein Engineering , MedImmune , Gaithersburg , MD , USA., Masterson L; b Spirogen Ltd , QMB Innovation Center , London , UK., Ruddle BT; a Antibody Discovery and Protein Engineering , MedImmune , Gaithersburg , MD , USA., Zhong H; c Oncology Research , MedImmune , Gaithersburg , MD , USA., Fazenbaker C; c Oncology Research , MedImmune , Gaithersburg , MD , USA., Strout P; c Oncology Research , MedImmune , Gaithersburg , MD , USA., Rosenthal K; a Antibody Discovery and Protein Engineering , MedImmune , Gaithersburg , MD , USA., Reed M; d Biologics Safety Assessment , MedImmune , Gaithersburg , MD , USA., Muniz-Medina V; e Biosuperiors , MedImmune , Gaithersburg , MD , USA., Howard P; b Spirogen Ltd , QMB Innovation Center , London , UK., Dixit R; d Biologics Safety Assessment , MedImmune , Gaithersburg , MD , USA., Wu H; a Antibody Discovery and Protein Engineering , MedImmune , Gaithersburg , MD , USA., Hinrichs MJ; d Biologics Safety Assessment , MedImmune , Gaithersburg , MD , USA., Gao C; a Antibody Discovery and Protein Engineering , MedImmune , Gaithersburg , MD , USA., Dimasi N; a Antibody Discovery and Protein Engineering , MedImmune , Gaithersburg , MD , USA.

Publikováno v: MAbs [MAbs] 2019 Apr; Vol. 11 (3), pp. 500-515. Date of Electronic Publication: 2019 Mar 05.