Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Ruben H. Hovhannisyan"'
Publikováno v:
BioTechniques, Vol 46, Iss 2, Pp 95-98 (2009)
Use of RNA affinity chromatography is commonly used to identify RNA binding proteins that interact with specific RNA cis-elements that function in post-transcriptional gene regulation. These purifications can be complicated by residual RNase activity
Externí odkaz:
https://doaj.org/article/f65c3cabb461479db14c0d8bad45924a
Autor:
Stephen Stephan, Ruben H. Hovhannisyan, Muredach P. Reilly, Rexford S. Ahima, Arbansjit Sandhu, Julie Johnston, Sean O’Neill, William R. Lagor, Shu-Jen Chen, Christine C. Hinkle
Publikováno v:
Gene therapy
Adipose tissue has a critical role in energy and metabolic homeostasis, but it is challenging to adapt techniques to modulate adipose function in vivo. Here we develop an in vivo, systemic method of gene transfer specifically targeting adipose tissue
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f10386c241acea66b48356fc6bd24e1
http://europepmc.org/articles/PMC4342115
http://europepmc.org/articles/PMC4342115
Publikováno v:
Journal of Biological Chemistry. 282:36265-36274
Splicing of fibroblast growth factor receptor 2 (FGFR2) alternative exons IIIb and IIIc is regulated by the auxiliary RNA cis-element ISE/ISS-3 that promotes splicing of exon IIIb and silencing of exon IIIc. Using RNA affinity chromatography, we have
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18ef96eaf45f541c79b671f41bac18c4
https://doi.org/10.1074/jbc.m704188200
https://doi.org/10.1074/jbc.m704188200
Publikováno v:
Nucleic Acids Research
Alternative splicing of fibroblast growth factor receptor-2 (FGFR2) mutually exclusive exons IIIb and IIIc results in highly cell-type-specific expression of functionally distinct receptors, FGFR2-IIIb and FGFR2-IIIc. We previously identified an RNA
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cb9f2877e67c41d0d6092e26477a4cc
http://europepmc.org/articles/PMC1331989
http://europepmc.org/articles/PMC1331989
Publikováno v:
Molecular and Cellular Biology. 25:250-263
Mutually exclusive splicing of fibroblast growth factor receptor 2 (FGFR2) exons IIIb and IIIc yields two receptor isoforms, FGFR2-IIIb and -IIIc, with distinctly different ligand binding properties. Several RNA cis elements in the intron (intron 8)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3103a8817e28ec67a3e421cffb38e076
https://doi.org/10.1128/mcb.25.1.250-263.2005
https://doi.org/10.1128/mcb.25.1.250-263.2005
Autor:
James M. Wilson, Arbans Sandhu, H. Lee Sweeney, Ruben H. Hovhannisyan, Odella Jno-Charles, Lawrence T. Bish, Shu-Jen Chen, Julie Johnston
Publikováno v:
Human gene therapy methods. 24(4)
The ability to regulate both the timing and specificity of gene expression mediated by viral vectors will be important in maximizing its utility. We describe the development of an adeno-associated virus (AAV)-based vector with tissue-specific gene re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9c83d9c76b9dca3052a248673f23f3e8
https://pubmed.ncbi.nlm.nih.gov/23895325
https://pubmed.ncbi.nlm.nih.gov/23895325
Publikováno v:
Methods in Molecular Biology ISBN: 9781617797668
Alternative splicing of pre-mRNA transcripts is a critical and extensively utilized mechanism of gene regulation. In this chapter, we describe a series of fluorescent and luminescent minigene reporters our lab has used to facilitate the study of alte
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9ccfa677d46dd2238b7f2c8333a93628
https://doi.org/10.1007/978-1-61779-767-5_18
https://doi.org/10.1007/978-1-61779-767-5_18
Autor:
Richard B. Jones, Stephanie J. Muh, Ruben H. Hovhannisyan, Emily A. Newman, Claude C. Warzecha, Wallace L. McKeehan, Russ P. Carstens
We have developed a series of fluorescent splicing reporter minigenes for the establishment of cell-based screens to identify splicing regulatory proteins. A key technical advance in the application of these reporters was the use of two different flu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68bcf775c277284bc29c044bcb3edf0b
https://europepmc.org/articles/PMC1464843/
https://europepmc.org/articles/PMC1464843/
Publikováno v:
The Journal of biological chemistry. 277(51)
Alternative splicing of fibroblast growth factor receptor 2 (FGFR2) mutually exclusive exons IIIb and IIIc represents a tightly regulated and functionally relevant example of post-transcriptional gene regulation. Rat prostate cancer DT3 and AT3 cell
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3ba961dcedc8a0b0ba7c537143793d6
https://pubmed.ncbi.nlm.nih.gov/12393912
https://pubmed.ncbi.nlm.nih.gov/12393912