Zobrazeno 1 - 10
of 214
pro vyhledávání: '"Ruben G Carbonell"'
Publikováno v:
PLoS ONE, Vol 7, Iss 11, p e48928 (2012)
We have engineered pH sensitive binding proteins for the Fc portion of human immunoglobulin G (hIgG) (hFc) using two different strategies - histidine scanning and random mutagenesis. We obtained an hFc-binding protein, Sso7d-hFc, through mutagenesis
Externí odkaz:
https://doaj.org/article/a40ba8300b354f0b861397f968075638
Autor:
Jinxin Fan, Eduardo Barbieri, Shriarjun Shastry, Stefano Menegatti, Cristiana Boi, Ruben G. Carbonell
Publikováno v:
Membranes, Vol 12, Iss 10, p 944 (2022)
The success of adeno-associated virus (AAV)-based therapeutics in gene therapy poses the need for rapid and efficient processes that can support the growing clinical demand. Nonwoven membranes represent an ideal tool for the future of virus purificat
Externí odkaz:
https://doaj.org/article/e7d1bf31cc7f4008b7d1dc1804adc49b
Publikováno v:
Membranes, Vol 11, Iss 7, p 530 (2021)
There is strong need to reduce the manufacturing costs and increase the downstream purification efficiency of high-value therapeutic monoclonal antibodies (mAbs). This paper explores the performance of a weak cation-exchange membrane based on the cou
Externí odkaz:
https://doaj.org/article/b733e90451dc4b88a00f3fbdb34fa35b
Publikováno v:
Membranes, Vol 11, Iss 3, p 181 (2021)
This study presents the preparation and characterization of UV-grafted polybutylene terepthalate (PBT) ion exchange nonwoven membranes for chromatographic purification of biomolecules. The PBT nonwoven was functionalized with sulfonate and secondary
Externí odkaz:
https://doaj.org/article/b457c78c77504f79bc823a8c12cbe164
Autor:
Sobhana Alekhya Sripada, Wenning Chu, Taufika Islam Williams, Matthew A. Teten, Brian J. Mosley, Ruben G. Carbonell, Abraham M. Lenhoff, Steven M. Cramer, Jerome Bill, Yinges Yigzaw, David J. Roush, Stefano Menegatti
Publikováno v:
Biotechnology and Bioengineering. 119:1873-1889
The growth of advanced analytics in manufacturing monoclonal antibodies (mAbs) has highlighted the challenges associated with the clearance of host cell proteins (HCPs). Of special concern is the removal of "persistent" HCPs, including immunogenic an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7635790d7340f8d2099455bdb13d469
https://doi.org/10.1002/bit.28096
https://doi.org/10.1002/bit.28096
Autor:
Jinxin Fan, Sobhana A. Sripada, Dan N. Pham, Marina Y. Linova, John M. Woodley, Stefano Menegatti, Cristiana Boi, Ruben G. Carbonell
Publikováno v:
Separation and Purification Technology. 317:123920
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bfa4dca4d40eaa11a9571a33a53b2ff5
https://doi.org/10.1016/j.seppur.2023.123920
https://doi.org/10.1016/j.seppur.2023.123920
Autor:
Sandeep B. Kedia, Jeffrey C. Baker, Ruben G. Carbonell, Kelvin H. Lee, Christopher J. Roberts, John Erickson, John E. Schiel, Kelley Rogers, Gene Schaefer, Stefanie Pluschkell
Publikováno v:
Biotechnology and bioengineeringREFERENCES. 119(12)
The Manufacturing Readiness Levels (MRLs) developed by the Department of Defense are well-established tools for describing the maturity of new technologies resulting from government-sponsored Research and Development programs, from the concept phase
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eadf81efb21651733c9e2c7c8eeb72d4
https://pubmed.ncbi.nlm.nih.gov/36071569
https://pubmed.ncbi.nlm.nih.gov/36071569
Autor:
Rudra Palash Mukherjee, Geok-Yong Yow, Samuel Sarakbi, Stefano Menegatti, Patrick V. Gurgel, Ruben G. Carbonell, Benjamin G. Bobay
Publikováno v:
Computational biology and chemistry. 102
Butyrylcholinesterase (BChE) is recognized as a high value biotherapeutic in the treatment of Alzheimer's disease and drug addiction. This study presents the rational design and screening of an in-silico library of trimeric peptides against BChE and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d024d33c0a1d39d0ac914820d2f79d78
https://pubmed.ncbi.nlm.nih.gov/36463785
https://pubmed.ncbi.nlm.nih.gov/36463785
Autor:
Ryan Kilgore, Wenning Chu, Dipendra Bhandari, David Fischler, Ruben G. Carbonell, Michael Crapanzano, Stefano Menegatti
Publikováno v:
Journal of Chromatography A. 1687:463701
Engineered multi-specific monoclonal antibodies (msAbs) and antibody fragments offer valuable therapeutic options against metabolic disorders, aggressive cancers, and viral infections. The advancement in molecular design and recombinant expression of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50afb5997661db075f4a58518654e5d3
https://doi.org/10.1016/j.chroma.2022.463701
https://doi.org/10.1016/j.chroma.2022.463701
Autor:
Tuhidul Islam, Yuki Ohara, Stefano Menegatti, Amith D. Naik, Takaaki Terasaka, Yasuhiro Hashimoto, Ruben G. Carbonell
Publikováno v:
Biochemical Engineering Journal. 145:53-61
A study is presented on the use of porous silica resins with tailored properties to develop affinity adsorbents for the purification of immunoglobulin G (IgG). Chromatorex® silica resins were utilized to study the dependence of IgG binding upon func
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::81bee922a2443f0fb388d47afb0acf67
https://doi.org/10.1016/j.bej.2018.07.011
https://doi.org/10.1016/j.bej.2018.07.011