Engineered Cas12i2 is a versatile high-efficiency platform for therapeutic genome editing

Autor: Colin McGaw, Anthony J. Garrity, Gabrielle Z. Munoz, Jeffrey R. Haswell, Sejuti Sengupta, Elise Keston-Smith, Pratyusha Hunnewell, Alexa Ornstein, Mishti Bose, Quinton Wessells, Noah Jakimo, Paul Yan, Huaibin Zhang, Lauren E. Alfonse, Roy Ziblat, Jason M. Carte, Wei-Cheng Lu, Derek Cerchione, Brendan Hilbert, Shanmugapriya Sothiselvam, Winston X. Yan, David R. Cheng, David A. Scott, Tia DiTommaso, Shaorong Chong

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-11 (2022)

Cas12i is a genome editing platform with compact size that fits in AAV vector with short 43-mer gRNA, absence of tracrRNA, ability to process pre-crRNA, and high specificity. Here the authors present an unbiased mutational scanning approach to engine

Externí odkaz: https://doaj.org/article/b34236ad23cc4e1cb4a7c8a576cc399b

Single-step assembly of asymmetric vesicles

Autor: Shin-Hyun Kim, David A. Weitz, Stephan A. Koehler, Juan L. Aragones, Laura R. Arriaga, Yuting Huang, Roy Ziblat

Publikováno v: Lab on a chip. 19(5)

Asymmetric vesicles are membranes in which amphiphiles are asymmetrically distributed between each membrane leaflet. This asymmetry dictates chemical and physical properties of these vesicles, enabling their use as more realistic models of biological

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2735a7773462060d346f0bfdf574e423
https://pubmed.ncbi.nlm.nih.gov/30672918

Regularized lattice Boltzmann multicomponent models for low capillary and Reynolds microfluidics flows

Autor: Marco Lauricella, Elad Stolovicki, Sauro Succi, Michele La Rocca, Roy Ziblat, Andrea Montessori, David A. Weitz

Publikováno v: Computers & Fluids
Computers & fluids 167 (2018): 33–39. doi:10.1016/j.compfluid.2018.02.029
info:cnr-pdr/source/autori:Montessori, Andrea; Lauricella, Marco; La Rocca, Michele; Succi, Sauro; Stolovicki, Elad; Ziblat, Roy; Weitz, David/titolo:Regularized lattice Boltzmann multicomponent models for low capillary and Reynolds microfluidics flows/doi:10.1016%2Fj.compfluid.2018.02.029/rivista:Computers & fluids/anno:2018/pagina_da:33/pagina_a:39/intervallo_pagine:33–39/volume:167

We present a regularized version of the color gradient lattice Boltzmann (LB) scheme for the simulation of droplet formation in microfluidic devices of experimental relevance. The regularized version is shown to provide computationally efficient acce

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::970ec2ec550995cd3e9ad9f10f085de6

Kristalline Lipiddomänen: Charakterisierung durch Röntgenbeugung und ihre Rolle in der Biologie

Autor: Leslie Leiserowitz, Roy Ziblat, Lia Addadi

Publikováno v: Angewandte Chemie. 123:3700-3710

Biologische Membranen enthalten Tausende verschiedenartiger Lipide, die sich in ihren Alkylketten, ihren Kopfgruppen und ihrem Sattigungsgrad unterscheiden. Es wird geschatzt, dass 5 % der Gene des menschlichen Genoms fur die Regulation der Lipidzusa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::2c8846a6db7454cdd1d9d885a6d5144d
https://doi.org/10.1002/ange.201004470

Two and Three-Dimensional Pattern Recognition of Organized Surfaces by Specific Antibodies

Autor: Roy Ziblat, Noa Rubin, Luana Scheffer, Lia Addadi

Publikováno v: Accounts of Chemical Research. 41:254-264

Understanding molecular recognition of supramolecules for solid substrates is essential for designing chemical sensors and molecular devices. The rules of molecular recognition are well established at the level of single molecules. However, during th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68ea3b170eaea56108aaa63ec4fd34c2
https://doi.org/10.1021/ar700153u