Zobrazeno 1 - 10
of 221
pro vyhledávání: '"Roumen Tsanev"'
Publikováno v:
Biotechnology & Biotechnological Equipment. 21:177-181
The effect of temperature, mechanical stress and surface tension on fluorescence and biological activity of recombinant human interferon gamma (rhIFN-γ) was studied. All three physical factors caused a similar decrease of fluorescence intensity and
Publikováno v:
Biotechnology & Biotechnological Equipment. 21:215-219
Equations were derived describing the fluorescence intensity of IFNy solutions on the basis of a dimer-monomers equilibrium of the native protein. The experimental data were found to fit very well the theoretical values predicted by these equations.
Publikováno v:
Analytical Biochemistry. 308:178-181
Autor:
Victoria Dorovska-Taran, Raina Boteva, Benedetto Salvato, Theodora Zlateva, Antonie J. W. G. Visser, Roumen Tsanev
Publikováno v:
Biochemistry. 35:14825-14830
The biologically active form of interferon gamma is a dimer composed of two noncovalently bound identical polypeptide chains of 17 kDa each. In this study, it was found that dissociation of the dimer into monomers significantly reduced the fluorescen
Autor:
Roumen Tsanev, Zoya V Avramova
Publikováno v:
Chromosoma. 103:293-301
DNA accomplishes its biological function in a complex with nuclear proteins. A minor protein fraction has been found in chromatin which could not be dissociated from DNA by reagents abolishing non-covalent type of interactions. The controversy surrou
Autor:
Andrei D. Mirzabekov, Jordanka Zlatanova, Todor Banchev, Ljuba Srebreva, Roumen Tsanev, Sergei Bavykin
Publikováno v:
Proceedings of the National Academy of Sciences. 90:3918-3922
An immunochemical method for analyzing protein interactions with BrdUrd-substituted DNA was used to study binding of histones to nascent DNA in nuclei. The results indicate that in Ehrlich ascites tumor (EAT) cells, histone H1 deposits on newly repli
Publikováno v:
The EMBO Journal. 11:1941-1947
The present work examines the fate of nucleosomes after in vitro transcription of a 1400 bp DNA template containing the mouse alpha-globin sequences and the promoter of T7 RNA polymerase. Naked and nucleosome-bearing templates (containing about four
Autor:
Stefan Nonchev, Roumen Tsanev
Publikováno v:
Molecular Reproduction and Development. 25:72-76
The protamine to histone replacement in fertilized mouse eggs was studied by using antibodies to these proteins. Its course was followed with respect to DNA replication by autoradiography of 3H-thymidine-labeled fertilized eggs. It was found that pro
Autor:
Roumen Tsanev, Ivan Ivanov
Publikováno v:
Immune Interferon ISBN: 9780849311482
Immune Interferon: Properties and Clinical Applications
Immune Interferon: Properties and Clinical Applications
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ac8573053e6b52b366b8d34f9e91dc40
https://doi.org/10.1201/9781420040739.pt2
https://doi.org/10.1201/9781420040739.pt2
Autor:
Roumen Tsanev, Ivan Ivanov
Publikováno v:
Immune Interferon ISBN: 9780849311482
Immune Interferon: Properties and Clinical Applications
Immune Interferon: Properties and Clinical Applications
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6b8c55aede8c9bdc82738dce7cabad8b
https://doi.org/10.1201/9781420040739.ch8
https://doi.org/10.1201/9781420040739.ch8