Engineering a functional 1-deoxy-D-xylulose 5-phosphate (DXP) pathway in Saccharomyces cerevisiae

Autor: Eugene Antipov, Lishan Zhao, Peter Jackson, Pinghua Liu, Sheela Muley, Jack D. Newman, Kevin L. Dietzel, Jeremy LaBarge, James Kirby, Gale Wichmann, Jay D. Keasling, Shayin S. Gottlieb, Sara P. Gaucher, Nathan A. Moss, Rossana Chan, Edward E. K. Baidoo, Kristy Michelle Hawkins, Tina Mahatdejkul

Publikováno v: Kirby, J; Dietzel, KL; Wichmann, G; Chan, R; Antipov, E; Moss, N; et al.(2016). Engineering a functional 1-deoxy-D-xylulose 5-phosphate (DXP) pathway in Saccharomyces cerevisiae. Metabolic Engineering, 38, 494-503. doi: 10.1016/j.ymben.2016.10.017. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/38t56316

© 2016 International Metabolic Engineering Society Isoprenoids are used in many commercial applications and much work has gone into engineering microbial hosts for their production. Isoprenoids are produced either from acetyl-CoA via the mevalonate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46ecfd68c0223740ab350d7c893e62e1
https://doi.org/10.1016/j.ymben.2016.10.017

Optimization of a heterologous mevalonate pathway through the use of variant HMG-CoA reductases

Autor: Rossana Chan, Jay D. Keasling, Taek Soon Lee, Christopher J. Petzold, Dylan Chivian, John Haliburton, Tanveer S. Batth, David E. Garcia, Swapnil R. Chhabra, Alyssa M. Redding-Johanson, Gregory D. Friedland, Suzanne M. Ma

Publikováno v: Metabolic Engineering. 13:588-597

Expression of foreign pathways often results in suboptimal performance due to unintended factors such as introduction of toxic metabolites, cofactor imbalances or poor expression of pathway components. In this study we report a 120% improvement in th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e02deb23c88f2b1c257cf58fccc2eba
https://doi.org/10.1016/j.ymben.2011.07.001

Enhancing Terpene yield from sugars via novel routes to 1-deoxy-d-xylulose 5-phosphate

Autor: Minobu Nishimoto, George Wang, Joel Martin, Wendy Schackwitz, Rossana Chan, Jeffrey L. Fortman, Jay D. Keasling, Edward E. K. Baidoo, James Kirby, Ruthie W. N. Chow

Publikováno v: Applied and environmental microbiology, vol 81, iss 1

Terpene synthesis in the majority of bacterial species, together with plant plastids, takes place via the 1-deoxy- d -xylulose 5-phosphate (DXP) pathway. The first step of this pathway involves the condensation of pyruvate and glyceraldehyde 3-phosph

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3e8818605cde34eb3795104191f3c4a9
https://escholarship.org/uc/item/6p4387hq

Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol

Autor: Nilu Prasad, Eric J. Steen, Samuel L. Myers, Christopher J. Petzold, Mario Ouellet, Alyssa M. Redding, Rossana Chan, Jay D. Keasling

Publikováno v: Microbial Cell Factories
Microbial Cell Factories, Vol 7, Iss 1, p 36 (2008)

Background Increasing energy costs and environmental concerns have motivated engineering microbes for the production of "second generation" biofuels that have better properties than ethanol. Results and conclusion Saccharomyces cerevisiae was enginee

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::047e58b00c6e0fb99cfec6cf4a4c3580
https://doi.org/10.1186/1475-2859-7-36

Optimizing dosimetry with high-dose intracoronary gamma radiation (21 Gy) for patients with diffuse in-stent restenosis

Autor: Natalie Gevorkian, Rebecca Torguson, Larry R. White, Betty Wang, Daniel A. Canos, William O. Suddath, Augusto D. Pichard, Lowell F. Satler, Ron Waksman, Anh B. Bui, Rossana Chan, Kenneth M. Kent, Pramod K. Kuchulakanti

Publikováno v: Cardiovascular revascularization medicine : including molecular interventions. 6(3)

The efficacy of intracoronary gamma radiation (IRT-gamma) in reducing recurrent in-stent restenosis (ISR) is well established using doses of 14-18 Gy. We sought to examine whether an escalation in dose to 21 Gy is safe and confers additional benefit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be734812e687512429c3cfdbd8355e0b
https://pubmed.ncbi.nlm.nih.gov/16275606