Performance of parasitological and molecular techniques for the diagnosis and surveillance of gambiense sleeping sickness.

Autor: Dieudonné Mumba Ngoyi, Rosine Ali Ekangu, Marie France Mumvemba Kodi, Patient Pati Pyana, Fatima Balharbi, Mélanie Decq, Victor Kande Betu, Wim Van der Veken, Claude Sese, Joris Menten, Philippe Büscher, Veerle Lejon

Publikováno v: PLoS Neglected Tropical Diseases, Vol 8, Iss 6, p e2954 (2014)

OBJECTIVES:Recently, improvements have been made to diagnostics for gambiense sleeping sickness control but their performance remains poorly documented and may depend on specimen processing prior to examination. In a prospective study in the Democrat

Externí odkaz: https://doaj.org/article/ab161b6ce2c942d882a6ab8f1de2f91a

Efficacy study of novel diamidine compounds in a Trypanosoma evansi goat model.

Autor: Kirsten Gillingwater, Carlos Gutierrez, Arlene Bridges, Huali Wu, Stijn Deborggraeve, Rosine Ali Ekangu, Arvind Kumar, Mohamed Ismail, David Boykin, Reto Brun

Publikováno v: PLoS ONE, Vol 6, Iss 6, p e20836 (2011)

Three diamidines (DB 75, DB 867 and DB 1192) were selected and their ability to cure T. evansi experimentally infected goats was investigated. A toxicity assessment and pharmacokinetic analysis of these compounds were additionally carried out. Goats

Externí odkaz: https://doaj.org/article/2e6941434eff422b983015e36822cc05

Accordance and concordance of PCR and NASBA followed by oligochromatography for the molecular diagnosis ofTrypanosoma bruceiandLeishmania

Autor: Stijn Deborggraeve, Al-farazdag A. Saad, Thierry Laurent, Henk D. F. H. Schallig, Gerard J. Schoone, Piet A. Kager, Simone De Doncker, Rosine Ali Ekangu, Mariska M.G. Leeflang, George W. Lubega, Philippe Büscher, Sayda El Safi, Claire M. Mugasa, Frank L. Basiye

Publikováno v: Tropical Medicine & International Health. 15:800-805

Summary Objective To evaluate the repeatability and reproducibility of four simplified molecular assays for the diagnosis of Trypanosoma brucei spp. or Leishmania ssp. in a multicentre ring trial with seven participating laboratories. Methods The tes

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0d70cab7e60f989a9c5133459921cbc2
https://doi.org/10.1111/j.1365-3156.2010.02547.x

Phase II Evaluation of Sensitivity and Specificity of PCR and NASBA Followed by Oligochromatography for Diagnosis of Human African Trypanosomiasis in Clinical Samples from D.R. Congo and Uganda

Autor: George W. Lubega, Gerard J. Schoone, Rosine Ali Ekangu, Philippe Büscher, Henk D. F. H. Schallig, Stijn Deborggraeve, Claire M. Mugasa, Thierry Laurent, Enock Matovu

Publikováno v: PLoS Neglected Tropical Diseases
PLoS Neglected Tropical Diseases, 4(7). Public Library of Science
PLoS neglected tropical diseases, 4(7). Public Library of Science
PLoS Neglected Tropical Diseases, Vol 4, Iss 7, p e737 (2010)

Background The polymerase chain reaction (PCR) and nucleic acid sequence-based amplification (NASBA) have been recently modified by coupling to oligochromatography (OC) for easy and fast visualisation of products. In this study we evaluate the sensit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2783264931e1cbe153b70e102cbd0cb1
https://doi.org/10.1371/journal.pntd.0000737