Zobrazeno 1 - 10
of 27
pro vyhledávání: '"Rose Szittner"'
Publikováno v:
Enzyme and Microbial Technology. 32:186-193
Alkyl-substituted Sepharose 4B prepared by the glycidyl ether method with different degrees of substitution and alkyl chain length, have been used as a non-ionic matrix for immobilization of bacterial luciferases. Exposure of hydrophobic clusters in
Publikováno v:
Chemico-Biological Interactions. :29-38
The fatty aldehyde dehydrogenase (Vh-ALDH) isolated from the luminescent bacterium, Vibrio harveyi, differs from other aldehyde dehydrogenases in its high affinity for NADP(+). The binding of NADP(+) appears to arise from the interaction of the 2'-ph
Publikováno v:
Biochemistry. 37:16130-16138
The lux-specific myristoyl-ACP thioesterase from Vibrio harveyi contains four tryptophan residues, Trp23, Trp99, Trp186, and Trp213. Replacement of each of these residues with tyrosine by site-directed mutagenesis coupled with fluorescence and quench
Publikováno v:
Molecular Microbiology. 19:767-775
Analysis of Vibrio harveyi dark autoinducer mutants has demonstrated that the level of LuxR was much lower than that observed in wild-type cells. Complementation with luxR fully restored luminescence suggesting that the lux autoinducer may control ex
Autor:
D. M. Lawson, Stefano Ferri, Zygmunt S. Derewenda, Yunyi Wei, Laurence Serre, Edward A. Meighen, Rose Szittner, Urszula Derewenda
Publikováno v:
Biochemistry. 33:9382-9388
The crystal structure of a myristoyl acyl carrier protein specific thioesterase (C14ACP-TE) from a bioluminescent bacterium, Vibrio harveyi, was solved by multiple isomorphous replacement methods and refined to an R factor of 22% at 2.1-A resolution.
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 1158:137-145
Bacterial luciferases with over 70% sequence identity from the terrestrial species, Xenorhabdus luminescens, and the marine species, Vibrio harveyi, exhibit large differences in thermal stability (Szittner and Meighen, 1990, J. Biol. Chem. 265, 16581
Publikováno v:
Biochemical and Biophysical Research Communications. 176:541-548
The nucleotide sequences of the luxA and luxB genes coding for the alpha and beta subunits, respectively, of luciferase from Photobacterium phosphoreum have been determined. The predicted amino acid sequences of the alpha and beta subunits were shown
Autor:
Rose Szittner, Edward A. Meighen
Publikováno v:
Journal of Biological Chemistry. 265:16581-16587
The lux genes required for expression of luminescence have been cloned from a terrestrial bacterium, Xenorhabdus luminescens, and the nucleotide sequences of the luxA and luxB genes coding for the alpha and beta subunits of luciferase determined. The
Publikováno v:
The Biochemical journal. 385(Pt 2)
Bacterial luciferases (LuxAB) can be readily classed as slow or fast decay luciferases based on their rates of luminescence decay in a single turnover assay. Luciferases from Vibrio harveyi and Xenorhabdus (Photorhabdus) luminescens have slow decay r
Publikováno v:
Biochemistry. 43(11)
The recently proposed model for the bacteria luciferase-flavin mononucleotide complex identifies a number of critical intermolecular interactions that define a binding platform for the isoalloxazine ring of flavin [Lin, L. Y., Sulea, T., Szittner, R.