Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Rosario Vanella"'
Autor:
Rosario Vanella, Christoph Küng, Alexandre A. Schoepfer, Vanni Doffini, Jin Ren, Michael A. Nash
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-14 (2024)
Abstract Understanding the complex relationships between enzyme sequence, folding stability and catalytic activity is crucial for applications in industry and biomedicine. However, current enzyme assay technologies are limited by an inability to simu
Externí odkaz:
https://doaj.org/article/557062c21d184b79aad9b20a50a7c08d
Autor:
Joanan Lopez-Morales, Rosario Vanella, Elizabeth A. Appelt, Sarah Whillock, Alexandra M. Paulk, Eric V. Shusta, Benjamin J. Hackel, Chang C. Liu, Michael A. Nash
Publikováno v:
Small Science, Vol 3, Iss 12, Pp n/a-n/a (2023)
Yeast surface display (YSD) is a powerful tool in biotechnology that links genotype to phenotype. In this review, the latest advancements in protein engineering and high‐throughput screening based on YSD are covered. The focus is on innovative meth
Externí odkaz:
https://doaj.org/article/ddae0038718a4776a71f8c164540509e
Autor:
Joanan Lopez-Morales, Rosario Vanella, Tamara Utzinger, Valentin Schittny, Julia Hirsiger, Michael Osthoff, Christoph T. Berger, Yakir Guri, Michael A. Nash
Publikováno v:
iScience, Vol 26, Iss 5, Pp 106648- (2023)
Summary: The emergence of the SARS-CoV-2 Omicron variant altered patient risk profiles and shifted the trajectory of the COVID-19 pandemic. Therefore, sensitive serological tests capable of analyzing patient IgG responses to multiple variants in para
Externí odkaz:
https://doaj.org/article/acba1905787e4d8683cc950f4cf31e66
Publikováno v:
Biophysical Reports, Vol 2, Iss 1, Pp 100035- (2022)
Here, we present a method based on yeast surface display that allows for direct comparison between population-level cell adhesion strength and single-molecule receptor-ligand rupture mechanics. We developed a high-throughput yeast adhesion assay in w
Externí odkaz:
https://doaj.org/article/001372e5b3ae4ec090c892f3ee6a732c
Data underlying the figures in the publication ”Enzyme Cascade with Horseradish Peroxidase Readout for High-Throughput Screening and Engineering of Human Arginase‑1" inAnal Chem.2023, 95, 7150-7157. Data for figures from main text labelled as Fig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2b7eef810ad1b6e799deac3898a485f
https://zenodo.org/record/7920813
https://zenodo.org/record/7920813
Autor:
Rosario Vanella, Christoph Küng, Alexandre A. Schoepfer, Vanni Doffini, Jin Ren, Michael A. Nash
Understanding the complex relationships between enzyme sequence, folding stability and catalytic activity is crucial for applications in industry and biomedicine. However, current enzyme assay technologies are limited by an inability to simultaneousl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::eec9ac34d63d01c7b9a206445579678f
https://doi.org/10.1101/2023.02.24.529916
https://doi.org/10.1101/2023.02.24.529916
Autor:
Joanan Lopez-Morales, Rosario Vanella, Tamara Utzinger, Valentin Schittny, Julia Hirsiger, Michael Osthoff, Christoph Berger, Yakir Guri, Michael A. Nash
With numerous variations in the Spike protein, including concentrated mutations in the receptor-binding domain (RBD), the SARS-CoV-2 Omicron variant significantly shifted in the trajectory of the COVID-19 pandemic. To understand individual patient ri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::96e0f8a2abf93206a424e2f0540e3654
https://doi.org/10.1101/2023.02.17.23286074
https://doi.org/10.1101/2023.02.17.23286074
Publikováno v:
ACS Synthetic Biology, 12 (2)
Yeast surface display is a valuable tool for protein engineering and directed evolution; however, significant variability in the copy number (i.e., avidity) of displayed variants on the yeast cell wall complicates screening and selection campaigns. H
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::598e37fb49feae2ddc19a38bb1f2c3e3
Publikováno v:
Reaction Chemistry & Engineering
Engineering catalytic and biophysical properties of enzymes is an essential step en route to advanced biomedical and industrial applications. Here, we developed a high-throughput screening and directed evolution strategy relying on single-cell hydrog
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bb65eb16ef2977dd8f5667152ef590e
Autor:
Rosario Vanella, Gordana Kovacevic, Vanni Doffini, Jaime Fernández de Santaella, Michael A. Nash
Publikováno v:
Chemical Communications, 58 (15)
Enzyme engineering is an important biotechnological process capable of generating tailored biocatalysts for applications in industrial chemical conversion and biopharma. Typical enhancements sought in enzyme engineering and in vitro evolution campaig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e88f2058572040e531e5334a0dcf081f