Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Rosalyn J. Marchant"'
Autor:
Rosalyn J. Marchant, Andrew J. Racher, Mohamed B. Al-Fageeh, C. Mark Smales, Michèle F. Underhill
Publikováno v:
Molecular Biotechnology. 39:69-77
Previous work has shown that recombinant protein yield can be improved from in vitro-cultured mammalian cells by culturing at sub-physiological temperatures, although this effect is cell line and product dependent. The mechanism(s) by which low tempe
Publikováno v:
Biotechnology and Bioengineering. 93:829-835
There are a growing number of reports on the sub-physiological temperature culturing (
Publikováno v:
Journal of Biological Chemistry. 276:45622-45627
One of the rate-limiting steps in protein folding has been shown to be the cis-trans isomerization of proline residues, which is catalyzed by a range of peptidylprolyl cis-trans isomerases. To characterize the interaction between model peptides and t
Publikováno v:
Cells and Culture ISBN: 9789048134182
Reports show that the differentiation of a B cell into an ‘antibody factory’ is dependent upon the regulation of components of the unfolded protein response (UPR). The UPR consists of a highly evolved network of specific pathways in the endoplasm
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fdf50540f04e8a4273881bc31024b6c7
https://doi.org/10.1007/978-90-481-3419-9_76
https://doi.org/10.1007/978-90-481-3419-9_76
Autor:
Rosalyn J. Marchant, Michèle F. Underhill, Sarah J. Scott, C. Mark Smales, Mohamed B. Al-Fageeh
Publikováno v:
Cell Engineering ISBN: 9781402052514
The cold-shock response has now been studied in various organisms for several decades, but unlike the heat-shock response, the mechanisms involved in the response to sub-physiological temperatures are poorly understood. Despite this, in recent years
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3f0f676bfa06ed80aabc588b9c028587
https://doi.org/10.1007/1-4020-5252-9_6
https://doi.org/10.1007/1-4020-5252-9_6
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 308
The fate and effectiveness of a therapeutic protein product within a patient is, in part at least, determined by potential product variation that leads to differential activity and the half-life of the protein reagents in vivo, and these variations c
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::659710b6bbf0f008f0b55b823f11151d
https://doi.org/10.1385/1-59259-922-2:375
https://doi.org/10.1385/1-59259-922-2:375
Publikováno v:
Molecular biotechnology. 34(2)
There are a growing number of reports on the beneficial effects of subphysiological temperature in vitro culturing (27-35 degrees C) of mammalian cells on recombinant protein yield. However, this effect is not conserved across cell lines and target p
Autor:
Evdoxia, Gourbatsi, Mohamed B, Al-Fageeh, Rosalyn J, Marchant, Sarah J, Scott, Michèle F, Underhill, C Mark, Smales
Publikováno v:
Molecular biotechnology. 33(1)
The generation of cell lines stably expressing recombinant material is a lengthy process and there has thus been much interest in the use of transient expression systems to rapidly produce recombinant material. To achieve this, the DNA of interest mu
Autor:
Rosalyn J. Marchant, Mohamed B. Al-Fageeh, Michele F. Underhill, Andrew J. Racher, C. Mark Smales
Publikováno v:
Molecular Biotechnology. 49:277-277